A5062230094- Advanced Fluorescence Microscopy Techniques
- Autophagy in Disease and Therapy
- Cell Image Analysis Techniques
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Calcium signaling and nucleotide metabolism
- Spectroscopy Techniques in Biomedical and Chemical Research
- RNA modifications and cancer
- Cellular transport and secretion
- ATP Synthase and ATPases Research
- Photoacoustic and Ultrasonic Imaging
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Lysosomal Storage Disorders Research
- Image Processing Techniques and Applications
- Glaucoma and retinal disorders
- Cardiovascular Function and Risk Factors
- Photosynthetic Processes and Mechanisms
- Advanced Glycation End Products research
- Diatoms and Algae Research
- Slime Mold and Myxomycetes Research
- Neurological Disease Mechanisms and Treatments
- Viral Infections and Immunology Research
- Advanced Electron Microscopy Techniques and Applications
UiT The Arctic University of Norway
2012-2025
University Hospital of North Norway
2017-2025
Centre for Arctic Gas Hydrate, Environment and Climate
2022
The University of Sydney
2017
Royal North Shore Hospital
2017
Bergen Kommune
2012
Autophagy is a lysosome-dependent degradation system conserved among eukaryotes. The mammalian Atg1 homologues, Unc-51 like kinase (ULK) 1 and 2, are multifunctional proteins with roles in autophagy, neurite outgrowth, vesicle transport. ULK complex involved autophagy consists of ULK1, ULK2, ATG13, FIP200, ATG101. We have used pulldown peptide array overlay assays to study interactions between the six different ATG8 family proteins. Strikingly, addition ULK1 ATG13 FIP200 interacted human...
Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) complex and autophagy-specific class III phosphatidylinositol 3-kinase I (PtdIns3K-C1). The latter contains PIK3C3/VPS34, PIK3R4/VPS15, BECN1/Beclin 1 ATG14 phosphorylates to generate 3-phosphate (PtdIns3P). Here, we show that PIK3C3, BECN1 contain functional LIR motifs...
Abstract Segmenting subcellular structures in living cells from fluorescence microscope images is a ground truth (GT)-deficient problem. The microscopes’ three-dimensional blurring function, finite optical resolution due to light diffraction, pixel and the complex morphological manifestations of all contribute GT-hardness. Unsupervised segmentation approaches are quite inaccurate. Therefore, manual relying on heuristics experience remains preferred approach. However, this process tedious,...
The paradoxical exacerbation of cellular injury and death during reperfusion remains a problem in treatment myocardial infarction. Mitochondrial dysfunction plays key role the pathogenesis ischemia injury. Dysfunctional mitochondria can be removed by mitophagy, culminating their degradation within acidic lysosomes. Mitophagy is pivotal maintaining cardiac homeostasis emerges as potential therapeutic target. Here we employ beating human engineered heart tissue (EHT) to assess mitochondrial...
Histology involves the observation of structural features in tissues using a microscope. While diffraction-limited optical microscopes are commonly used histological investigations, their resolving capabilities insufficient to visualize details at subcellular level. Although novel set super-resolution microscopy techniques can fulfill resolution demands such cases, system complexity, high operating cost, lack multi-modality, and low-throughput imaging these methods limit wide adoption for...
In addition to splicing, group I intron RNA is capable of an alternative two-step processing pathway that results in the formation full-length circular RNA. The circularization initiated by hydrolytic cleavage at 3′ splice site and followed a transesterification reaction which terminal guanosine attacks 5′ presented structure analogous first step splicing. products reactions are unligated exons. For this reason, benefit expense host. has distinct structural requirements differ from those...
Mitochondria are susceptible to damage resulting from their activity as energy providers. Damaged mitochondria can cause harm the cell and thus subjected elaborate quality-control mechanisms including elimination via lysosomal degradation in a process termed mitophagy. Basal mitophagy is house-keeping mechanism fine-tuning number of according metabolic state cell. However, molecular underlying basal remain largely elusive. In this study, we visualized assessed level H9c2 cardiomyoblasts at...
Abstract This three-dimensional structured illumination microscopy (3DSIM) dataset was generated to highlight the suitability of 3DSIM investigate mitochondria-derived vesicles (MDVs) in H9c2 cardiomyoblasts living or fixed cells. MDVs act as a mitochondria quality control mechanism. The cells were stably expressing tandem-tag eGFP-mCherry-OMP25-TM (outer mitochondrial membrane) which can be used sensor for acidity. A part is showing correlative imaging lysosomes labeled using LysoTracker...
Multifocus microscopy enables recording of entire volumes in a single camera exposure. In dense samples, multifocus is severely hampered by background haze. Here, we introduce scalable method that incorporates optical sectioning and offers improved axial resolution capabilities. our method, dithered oblique light-sheet scans the sample volume during exposure, while fluorescence from each illuminated plane mapped onto line on with element. A synchronized rolling shutter readout realizes...
Mitochondria play a crucial role in cellular metabolism. This paper presents novel method to visualize mitochondria living cells without the use of fluorescent markers. We propose physics-guided deep learning approach for obtaining virtually labeled micrographs from bright-field images. integrate microscope’s point spread function an adversarial neural network improving virtual labeling. show results (average Pearson correlation 0.86) significantly better than what was achieved by...
The wide, but scattered distribution of group I introns in nature is a result two processes; the vertical inheritance with or without losses, and occasional transfer across species barriers. Reversal intron self-splicing reaction, termed reverse splicing, coupled transcription genomic integration potentially mediate an RNA-based mobility pathway. Compared to well characterized endonuclease-mediated homing, splicing less specific represents likely explanation for many transpositions into new...
The GIR1 branching ribozyme constitutes a separate class of naturally occurring ribozymes. Most studies have been performed with the single known from myxomycete Didymium iridis whereas large number GIR1s found in amoeboflagellate Naegleria has remained largely uncharacterized. Here, we investigate cleavage properties collection ribozymes and define variant N. pringsheimi as suitable model due to its superior activity vitro. We identify minimal by deletion analysis applying new RNase R based...
Detecting and analyzing nanoscale motion patterns of vesicles, smaller than the microscope resolution (~250 nm), inside living biological cells is a challenging problem. State-of-the-art CV approaches based on detection, tracking, optical flow or deep learning perform poorly for this We propose an integrative approach, built upon physics simulations, nanoscopy algorithms, shallow residual attention network to make it possible first time analysis sub-resolution in vesicles that may also be...
Mitochondria are essential energy-providing organelles of particular importance in energy-demanding tissue such as the heart. The production mitochondria-derived vesicles (MDVs) is a cellular mechanism by which cells ensure healthy pool mitochondria. These small and fast-moving objects not easily captured imaging. In this work, we have tested ability optical super-resolution technique 3DSIM to capture high-resolution images MDVs. We optimized imaging conditions both for high-speed video...
The quantitative analysis of subcellular organelles such as mitochondria in cell fluorescence microscopy images is a demanding task because the inherent challenges segmentation these small and morphologically diverse structures. In this article, we demonstrate use machine learning-aided pipeline for quantification mitochondrial morphology fixed cells. deep learning-based tool trained on simulated eliminates requirement ground truth annotations supervised learning. We utility cardiomyoblasts...
DiGIR2 is the group I splicing‐ribozyme of mobile twin‐ribozyme intron Dir.S956‐1, present in Didymium nuclear ribosomal DNA. responsible for excision, exon ligation, 3′‐splice site hydrolysis, and full‐length RNA circle formation. We recently reported that splicing (intron excision ligation) competes with hydrolysis subsequent circularization. Here we experimental evidence at dependent on structural elements within P9 subdomain not involved splicing. Whereas GCGA tetra‐loop P9b was found to...
Abstract RNA tertiary interactions involving docking of GNRA (N; any base; R; purine) hairpin loops into helical stem structures on other regions the same are one most common interactions. In this study, we investigated a association between GAAA tetraloop in small branching ribozyme (DiGIR1) and receptor motif (HEG P1 motif) present structure separate mRNA molecule. DiGIR1 generates 2′, 5′ lariat cap at end its downstream homing endonuclease by catalysing self-cleavage reaction an internal...