A5062389967- Advanced Fluorescence Microscopy Techniques
- Near-Field Optical Microscopy
- Photonic and Optical Devices
- Optical Coherence Tomography Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Digital Holography and Microscopy
- Microfluidic and Bio-sensing Technologies
- Advanced Electron Microscopy Techniques and Applications
- Plasmonic and Surface Plasmon Research
- Cell Image Analysis Techniques
- Advanced biosensing and bioanalysis techniques
- Optical measurement and interference techniques
- Advanced Biosensing Techniques and Applications
- Orbital Angular Momentum in Optics
- Photoacoustic and Ultrasonic Imaging
- Photonic Crystals and Applications
- Biosensors and Analytical Detection
- Bacterial Identification and Susceptibility Testing
- Nanofabrication and Lithography Techniques
- Molecular Biology Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Fiber Optic Sensors
- Advanced Photocatalysis Techniques
- Integrated Circuits and Semiconductor Failure Analysis
- Quantum Dots Synthesis And Properties
UiT The Arctic University of Norway
2014-2025
Centre for Arctic Gas Hydrate, Environment and Climate
2018-2023
SINTEF
2023
Oslo University Hospital
2023
Weatherford College
2023
Tromsø research foundation
2018
University of Graz
2011-2014
Vorarlberg University of Applied Sciences
2011
Swiss Federal Laboratories for Materials Science and Technology
2011
Antimicrobial resistance (AMR) is a major health threat worldwide and the culture-based bacterial detection methods are slow. Surface-enhanced Raman spectroscopy (SERS) can be used to identify target analytes in real time with sensitivity down single-molecule level, providing promising solution for culture-free detection. We report fabrication of SERS substrates having tightly packed silver (Ag) nanoparticles loaded onto long silicon nanowires (Si NWs) grown by metal-assisted chemical...
For plasmonic nanoparticles, we investigate the influence of surface roughness inherent to top-down fabrication on optical properties, and find that it has a surprisingly small position width plasmon peaks. Our experimental observation is supported by simulations based boundary element method approach. Using perturbation approach, suitable for metallic nanoparticles with moderate degree roughness, demonstrate reason this lies in motional narrowing where averages over random height...
Waveguide chip-based microscopy reduces the complexity of total internal reflection fluorescence (TIRF) microscopy, and adds features like large field view illumination, decoupling illumination collection path easy multimodal imaging. However, for technique to become widespread there is a need low-loss affordable waveguides made high-refractive index material. Here, we develop report silicon nitride (Si3N4) waveguide platform multi-color TIRF microscopy. Single mode conditions at visible...
Optical nanoscopy techniques can image intracellular structures with high specificity at sub-diffraction limited resolution, bridging the resolution gap between optical microscopy and electron microscopy. So far conventional lacks ability to generate throughput data, as imaged region is small. Photonic chip-based has demonstrated potential for imaging large areas, but a lateral of 130 nm. However, all existing super-resolution methods provide 100 nm or better. In this work, 75 over an...
In this article, we investigate the Surface-Enhanced Raman Scattering (SERS) efficiency of methylene blue (MB) molecules deposited on gold nanostripes which, due to their fabrication by electron beam lithography and thermal evaporation, present various degrees crystallinity nanoscale surface roughness (NSR). By comparing nanostructures with different crystallinity, show that NSR has a strong effect SERS intensity MB probe molecules. particular, features lithographic structures significantly...
Histology involves the observation of structural features in tissues using a microscope. While diffraction-limited optical microscopes are commonly used histological investigations, their resolving capabilities insufficient to visualize details at subcellular level. Although novel set super-resolution microscopy techniques can fulfill resolution demands such cases, system complexity, high operating cost, lack multi-modality, and low-throughput imaging these methods limit wide adoption for...
The lithographic fabrication of metal nanoparticles usually involves the thermal vacuum deposition metals, which leads to polycrystallinity and surface roughness. In recent years, strong efforts have been made clarify role such roughness in surface-enhanced Raman scattering (SERS). this paper, we provide a systematic experimental theoretical study single lithographically fabricated unravel morphology on optical far- near-field properties. We find that experimentally observed reduction SERS...
Red blood cells (RBCs) have the ability to undergo morphological deformations during microcirculation, such as changes in surface area, volume and sphericity. Optical waveguide trapping is suitable for trapping, propelling deforming large cell populations along length of waveguide. Bright field microscopy employed with does not provide quantitative information about structural changes. Here, we combined phase techniques study RBC morphology planar transportation. By using interference...
To demonstrate nanoscale motion tracing of spermatozoa and present analysis the traces to characterize consistency as a complement progressive motility analysis.
We present experimental demonstration of tilt-mirror assisted transmission structured illumination microscopy (tSIM) that offers a large field view super resolution imaging. An assembly custom-designed tilt-mirrors are employed as the module where sample is excited with interference two beams reflected from opposite pair mirror facets. Tunable frequency patterns generated by changing mirror-tilt angle and hexagonal-symmetric arrangement considered for isotropic in three orientations....
A new approach for the large-scale production of flexible photoelectrodes dye-sensitized solar cells (DSSCs) is presented by roll-to-roll coating a titanium dioxide nanodispersion containing block copolymer 'Pluronic®' (PEOx-PPOy-PEOx, PEO: poly(ethylene oxide), PPO: poly(propylene oxide)). Functional DSSCs were assembled and different procedures compared with respect to their power conversion efficiency. It shown that binder 'Pluronic' can be removed at processing temperatures as low 140...
Phase shifting interferometric (PSI) techniques are among the most sensitive phase measurement methods. Owing to its high sensitivity, any minute change caused due environmental instability results into, inaccurate measurement. Consequently, a well calibrated piezo electric transducer (PZT) and highly-stable environment is mandatory for measuring accurate map using PSI implementation. Here, we present an inverse approach, which can retrieve maps of samples with negligible errors under...
Abstract Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation on fixed cells [1,2] and also demonstrated short‐term live‐imaging robust cell types [3‐5], but not yet delicate primary neurons nor over extended periods time. Here, we present a waveguide‐based TIRFM set‐up live‐cell imaging demanding samples. Using the developed microscope, referred as ChipScope ,...
Visualization of three-dimensional (3D) morphological changes in the subcellular structures a biological specimen is major challenge life science. Here, we present an integrated chip-based optical nanoscopy combined with quantitative phase microscopy (QPM) to obtain 3D morphology liver sinusoidal endothelial cells (LSEC). LSEC have unique small nanopores (50-300 nm diameter) plasma membrane, called fenestrations. The fenestrations are grouped discrete clusters, which around 100 200 thick....
Abstract Applying structured instead of plane illumination in widefield optical fluorescence microscopy can improve the spatial resolution beyond what is known as Abbe limit. In general it not only an imaging system that interest but also its field view (FOV). These two parameters are expressed space-bandwidth product (SBP). Here we introduce a modified (SIM) approach offers larger SBP than any other available implementation. This achieved through transillumination geometry typical...
The single-mode limit and bending losses for shallow rib waveguides are studied using the full vectorial film mode matching method. maximum height is found to be on order of 10 nm a width 2 μm wavelength 785 nm, with exact value depending core thickness polarization. Bending calculated as function several geometrical parameters, both polarizations fundamental first modes. decrease significantly waveguides. For slightly larger heights, giving multimode waveguides, it that first-order orders...
Programmable nanoscale carriers, such as liposomes and DNA, are readily being explored for personalized medicine or disease prediction diagnostics. The characterization of these nanocarriers is limited challenging due to their complex chemical composition. Here, we demonstrate the utilization surface-enhanced Raman spectroscopy (SERS), which provides a unique molecular fingerprint analytes while reducing detection limit. In this paper, utilize silver coated nano-bowl shaped...
Abstract Correlative light and electron microscopy (CLEM) unifies the versatility of (LM) with high resolution (EM), allowing one to zoom into complex organization cells. Here, we introduce photonic chip assisted CLEM, enabling multi-modal total internal reflection fluorescence (TIRF) over large field view precision localization target area interest within EM. The chips are used as a substrate hold, illuminate provide landmarking sample through specially designed grid-like numbering systems....
Photonic chip-based total internal reflection fluorescence microscopy (c-TIRFM) is an emerging technology enabling a large TIRF excitation area decoupled from the detection objective. Additionally, due to inherent multimodal nature of wide waveguides, it convenient platform for introducing temporal fluctuations in illumination pattern. The fluctuation-based nanoscopy technique multiple signal classification algorithm (MUSICAL) does not assume stochastic independence emitter emission and can...
ABSTRACT The world health organization considers antimicrobial resistance (AMR) to be a critical global public problem. Conventional culture-based methods that are used detect and identify bacterial infection slow. Thus, there is growing need for the development of robust, cost-effective, fast diagnostic solutions identification pathogens. Surface-enhanced Raman spectroscopy (SERS) can target analytes with sensitivity down single-molecule level. Here, we developed SERS chip by optimizing...
Motility of cells plays an important role to determine the cell growth, health and monitor gene transformation. The aim current study is propose a combination high-contrast label-free imaging method computational approach (conventionally used for super-resolution) which can be as tool in tracing motion organelles. Here, we integrate quantitative phase microscopy (QPM) with waveguide-based trapping (WT) applied multiple signal classification algorithm (MUSICAL) analyze trapped particle. We...