A5088549373- Pregnancy and preeclampsia studies
- Advanced Fluorescence Microscopy Techniques
- Molecular Biology Techniques and Applications
- Birth, Development, and Health
- Blood groups and transfusion
- Advanced Biosensing Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Cell Image Analysis Techniques
- Platelet Disorders and Treatments
- Advanced Electron Microscopy Techniques and Applications
- Digital Holography and Microscopy
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Optical measurement and interference techniques
- Heparin-Induced Thrombocytopenia and Thrombosis
- Optical Coherence Tomography Applications
- Genomic variations and chromosomal abnormalities
- Wound Healing and Treatments
- Electrolyte and hormonal disorders
- Prenatal Screening and Diagnostics
- Meningioma and schwannoma management
- Micro and Nano Robotics
- Genetics and Neurodevelopmental Disorders
- Advanced biosensing and bioanalysis techniques
- Ubiquitin and proteasome pathways
UiT The Arctic University of Norway
2011-2024
University Hospital of North Norway
2007-2024
Centre for Arctic Gas Hydrate, Environment and Climate
2023
The rather limited success of translation from basic research to clinical application has been highlighted as a major issue in the nanomedicine field. To identify factors influencing applicability nanosystems drug carriers and potential nanomedicine, we focused on following their fate through fluorescence-based assays, namely flow cytometry imaging. These methods are often used follow nanocarrier internalization targeting; however, validity obtained results strictly depends how much...
Histology involves the observation of structural features in tissues using a microscope. While diffraction-limited optical microscopes are commonly used histological investigations, their resolving capabilities insufficient to visualize details at subcellular level. Although novel set super-resolution microscopy techniques can fulfill resolution demands such cases, system complexity, high operating cost, lack multi-modality, and low-throughput imaging these methods limit wide adoption for...
Meningiomas represent the most common primary tumors of central nervous system, but few microRNA (miRNA) profiling studies have been reported so far. Deep sequencing small RNA libraries generated from two human meningioma biopsies WHO grades I (benign) and II (atypical) were compared to excess dura controls. Nineteen differentially expressed miRNAs validated by RT-qPCR using tumor 15 patients 5 meninges Tumor suppressor miR-218 miR-34a upregulated relative normal controls, however, miR-143,...
Infected chronic skin wounds and other infections are increasingly putting pressure on the health care providers patients. The is especially concerning due to rise of antimicrobial resistance biofilm-producing bacteria that further impair treatment success. Therefore, innovative strategies for wound healing bacterial eradication urgently needed; utilization materials with inherent biological properties could offer a potential solution. Chitosan one most frequently used polymers in delivery...
Maternal alloimmunization against human platelet antigen (HPA)-1a has been implied to mediate both reduced birth weight and chronic placental inflammation. Fetal growth restriction is associated with different types of inflammation in the placenta, mainly histiocytic intervillositis villitis. The aim this prospective study was do a systematic examination placentas from HPA-1a alloimmunized pregnancies, focus on histopathological immunohistochemical diagnosis variants In Polish-Norwegian...
High space-bandwidth product with high spatial phase sensitivity is indispensable for a single-shot quantitative microscopy (QPM) system. It opens avenue widespread applications of QPM in the field biomedical imaging. Temporally low coherence light sources are implemented to achieve at cost either reduced temporal resolution or smaller view (FOV). In addition, such have photon degeneracy. On contrary, like lasers capable exploiting full FOV systems expense less sensitivity. present work, we...
To demonstrate nanoscale motion tracing of spermatozoa and present analysis the traces to characterize consistency as a complement progressive motility analysis.
Super-resolution fluorescence microscopy is a widely employed technique in cell biology research, yet remains relatively unexplored the field of histopathology. Here, we describe sample preparation steps and acquisition parameters necessary to obtain fluorescent multicolor super-resolution structured illumination (SIM) images both formalin-fixed paraffin-embedded cryo-preserved placental tissue sections. We compare super-resolved chorionic villi against diffraction-limited deconvolution show...
Background Hydrops fetalis, a pathological fluid accumulation in two or more body compartments, is aetiologically heterogeneous. We investigated consanguineous family with recurrent pregnancy loss due to severe early-onset non-immune hydrops fetalis. Methods and results Whole exome sequencing four fetuses fetalis revealed that they were homozygous for the angiopoietin-2 ( ANGPT2 ) variant Chr8 (GRCh37/Hg19): 6385085T>C, NM_001147.2:c.557A>G. The substitution introduces cryptic, exonic...
Laeverin is a placenta-specific membrane-bound aminopeptidase. In this study we wanted to: 1) serially measure plasma levels of laeverin in healthy women during the second half pregnancy and postpartum, 2) determine whether differently expressed at 22-24 weeks who later develop preeclampsia compared to controls, 3) compare protein expression placenta umbilical vein serum preeclamptic pregnancies birth.Plasma was obtained serially, approximately every 4-weeks, from 53 with uncomplicated 22+0...
Laeverin is a placenta-specific protein that normally expressed in the plasma membrane of human trophoblasts. In previous studies, we showed higher expression levels laeverin gene preeclamptic compared with normal placentas and found was ectopically cytoplasm placentas. Our objective to investigate combining immunohistochemistry immunofluorescence.Tissue microarray analysis 72 placentas, obtained from 33 39 uncomplicated pregnancies, performed. labeled specific antibody for...
Objective: The aim of this study was to compare the laeverin level in maternal serum from first trimester (11-14 weeks) pregnancy between normal pregnancies and that later developed preeclampsia (PE). Material methods: This a case-cohort study. concentration measured cases with preterm PE (n = 55), term 95), reference group randomly selected women outcome 200) stored samples collected double-test as part combined trisomy 21 screening program. were thawed analyzed for laeverin. median...
Conventional methods of assessing sperm quality are based on qualitative analysis semen. In this paper, we combined time-lapse quantitative phase imaging and deep learning for the classification healthy unhealthy cells.
Histology is a well-known examination technique to study the biological cell and tissue structures. For histological assessment, imaging throughput, contrast, resolution, quantification of morphology are crucial parameters. Although, there techniques available which can scan whole slide, but they lack specificity quantification. In present study, we introduce photonic chip based platform for multimodal FFPE sections. Here, was integrated with Linnik type QPM module, enables high contrast...
Abstract Fluorescence-based super-resolution optical microscopy (SRM) techniques allow the visualization of biological structures beyond diffraction limit conventional microscopes. Despite its successful adoption in cell biology, integration SRM into field histology has been deferred due to several obstacles. These include limited imaging throughput, high cost, and need for complex sample preparation. Additionally, refractive index heterogeneity labeling density commonly available...
Abstract Super-resolution fluorescence microscopy is a widely employed technique in cell biology research, yet remains relatively unexplored the field of histo-pathology. Here, we describe sample preparation steps and acquisition parameters necessary to obtain fluorescent multicolor super-resolution structured illumination (SIM) images both formalin-fixed paraffin-embedded cryo-preserved placental tissue sections. We compare super-resolved chorionic villi against diffraction-limited...