Antimicrobial resistance (AMR) is a major health threat worldwide and the culture-based bacterial detection methods are slow. Surface-enhanced Raman spectroscopy (SERS) can be used to identify target analytes in real time with sensitivity down single-molecule level, providing promising solution for culture-free detection. We report fabrication of SERS substrates having tightly packed silver (Ag) nanoparticles loaded onto long silicon nanowires (Si NWs) grown by metal-assisted chemical...

In this Letter, we demonstrate quantitative phase imaging of biological samples, such as human red blood cells (RBCs) and onion using narrow temporal frequency wide angular spectrum light source. This type source was synthesized by the combined effect spatial, angular, diversity speckle reduction technique. The importance low spatial high coherence over broad band is that it does not require any dispersion compensation mechanism for samples. Further, avoids formation or spurious fringes...

Semen quality assessed by sperm count and cell characteristics such as morphology motility, is considered to be the main determinant of men's reproductive health. Therefore, selection vital in assisted technology (ART) used for treatment infertility. Conventional bright field optical microscopy widely utilized imaging cells based on qualitative analysis experienced clinicians. In this study, we report development a highly sensitive quantitative phase (QPM) using partially spatially coherent...

We report multispectral phase-shifting interference microscopy for quantitative phase imaging of human red blood cells (RBCs). A wide range wavelengths are covered by means using multiple color light emitting diodes (LEDs) with narrow spectral bandwidth ranging from violet to deep color. The multicolor LED source was designed and operated sequentially, which works as a scanning source. Corresponding each source, five phase-shifted interferograms were recorded sequentially the measurement...

We demonstrate ultra-high axial-resolution topography and tomography of multilayered objects using pseudo thermal light source, i.e., laser. The longitudinal spatial coherence (LSC) length was significantly reduced by synthesizing a source with the combined effect spatial, angular, temporal diversity. Thus, generating low spatially coherent (i.e., broad angular frequency spectrum) having narrow spectrum. LSC less than 10 μm very magnification lens. Experimental results optical sectioning...

Histology involves the observation of structural features in tissues using a microscope. While diffraction-limited optical microscopes are commonly used histological investigations, their resolving capabilities insufficient to visualize details at subcellular level. Although novel set super-resolution microscopy techniques can fulfill resolution demands such cases, system complexity, high operating cost, lack multi-modality, and low-throughput imaging these methods limit wide adoption for...

In breast cancer, 20%-30% of cases require a second surgery because incomplete excision malignant tissues. Therefore, to avoid the risk recurrence, accurate detection cancer margin by clinician or surgeons needs some assistance. this paper, an automated volumetric analysis normal and tissue is done machine learning algorithm separate them into two classes. The proposed method based on support-vector-machine-based classifier dissociating 10 features extracted from A-line, texture, phase map...

Red blood cells (RBCs) have the ability to undergo morphological deformations during microcirculation, such as changes in surface area, volume and sphericity. Optical waveguide trapping is suitable for trapping, propelling deforming large cell populations along length of waveguide. Bright field microscopy employed with does not provide quantitative information about structural changes. Here, we combined phase techniques study RBC morphology planar transportation. By using interference...

In the present Letter, a synthesized pseudothermal light source having high temporal coherence (TC) and low spatial (SC) properties is used. The longitudinal (LC) of spatially extended monochromatic are systematically studied. generated from two different laser sources: He-Ne (at 632 nm) DPSS 532 nm). It was found that LC length such becomes independent parent laser's TC for large size. For chosen lasers, constant to about 30 μm size ≥3.3 mm. Thus, by appropriately choosing size, any...

Optical coherence tomography (OCT) is being increasingly adopted as a label-free and non-invasive technique for biomedical applications such cancer ocular disease diagnosis. Diagnostic information these tissues manifest in textural geometric features of the OCT images, which are used by human expertise to interpret triage. However, it suffers delays due long process conventional diagnostic procedure shortage expertise. Here, custom deep learning architecture, LightOCT, proposed...

Total internal reflection fluorescence (TIRF) microscopy benefits from high-sensitivity, low background noise, photo-toxicity and high-contrast imaging of sub-cellular structures close to the membrane surface. Although, TIRF provides it does not provide quantitative information about morphological features biological cells. Here, we propose an integrated waveguide chip-based label-free phase (QPI). The evanescent field present on top a surface is used excite upright microscope collect...

Quantitative phase microscopy (QPM) is a label-free technique that enables to monitor morphological changes at subcellular level. The performance of the QPM system in terms spatial sensitivity and resolution depends on coherence properties light source numerical aperture (NA) objective lenses. Here, we propose high space-bandwidth using partially spatially coherent optical (PSC-OCM) assisted with deep neural network. PSC synthesized improve reconstructed map from interferometric images....

High space-bandwidth product with high spatial phase sensitivity is indispensable for a single-shot quantitative microscopy (QPM) system. It opens avenue widespread applications of QPM in the field biomedical imaging. Temporally low coherence light sources are implemented to achieve at cost either reduced temporal resolution or smaller view (FOV). In addition, such have photon degeneracy. On contrary, like lasers capable exploiting full FOV systems expense less sensitivity. present work, we...

Early-stage detection of breast cancer is the primary requirement in modern healthcare as it most common among women worldwide. Histopathology widely preferred method for diagnosis cancer, but requires long processing time and involves qualitative assessment by a trained person/doctor. Here, we present an alternate technique based on white light interference microscopy (WLIM) Raman spectroscopy, which has capability to differentiate between cancerous normal tissue. WLIM provides quantitative...

Phase shifting interferometric (PSI) techniques are among the most sensitive phase measurement methods. Owing to its high sensitivity, any minute change caused due environmental instability results into, inaccurate measurement. Consequently, a well calibrated piezo electric transducer (PZT) and highly-stable environment is mandatory for measuring accurate map using PSI implementation. Here, we present an inverse approach, which can retrieve maps of samples with negligible errors under...

Multi-spectral quantitative phase imaging (QPI) is an emerging modality for wavelength dependent studies of several biological and industrial specimens.Simultaneous multi-spectral QPI generally performed with color CCD cameras.Here, we present a new approach accurately measuring the crosstalk 2D area detectors, without needing prior information about camera specifications.Color systematically studied compared using compact interference microscopy on two different cameras commonly used in...

Current low coherence quantitative phase microscopy (LC-QPM) systems suffer from either reduced field of view (FoV) or temporal resolution due to the short (TC) length light source. Here, we propose a hybrid, experimental and numerical approach address this core problem associated with LC-QPM. We demonstrate high spatial sensitivity in LC-QPM at resolution. High space-time bandwidth product is achieved by employing incoherent source for sample illumination QPM increase single-shot Hilbert...

We developed a new quantitative phase microscopy technique, namely, spectrally resolved laser interference (SR-LIM), with which it is possible to quantify multi-spectral information related biological specimens without color crosstalk using CCD camera. It single shot technique where sequential switched on/off of red, green, and blue (RGB) wavelength light sources are not required. The method implemented three-wavelength microscope customized compact grating based imaging spectrometer fitted...

The article elucidates the physical mechanism behind generation of superior-contrast and high-resolution label-free images using an optical waveguide. Imaging is realized by employing a high index contrast multi-moded waveguide as partially coherent light source. modes provide near-field illumination unlabeled samples, thereby repositioning higher spatial frequencies sample into far-field. These coherently scatter off with different phases are engineered to have random distributions within...

In pregnancy during an inflammatory condition, macrophages present at the feto-maternal junction release increased amount of nitric oxide (NO) and pro-inflammatory cytokines such as TNF-α INF-γ, which can disturb trophoblast functions outcome. Measurement cellular sub-cellular morphological modifications associated with responses are important in order to quantify extent dysfunction for clinical implication. With this motivation, we investigated morphological, changes externally inflamed...

ABSTRACT The world health organization considers antimicrobial resistance (AMR) to be a critical global public problem. Conventional culture-based methods that are used detect and identify bacterial infection slow. Thus, there is growing need for the development of robust, cost-effective, fast diagnostic solutions identification pathogens. Surface-enhanced Raman spectroscopy (SERS) can target analytes with sensitivity down single-molecule level. Here, we developed SERS chip by optimizing...

We report three-dimensional surface profilometry with extended range of height measurements using synthetic wavelength scanning interferometry without tunable filters, wavelength-tuning lasers, grating elements. have used inexpensive multiple colour light emitting diodes (LEDs) and operate them sequentially one by or combination two more colours simultaneously to visualize source. Multiple LED source was synthesized entire visible from violet deep red covered. A wide wavelengths were...

We report white light phase shifting interferometry in conjunction with color fringe analysis for the detection of contaminants water such as Escherichia coli (E.coli), Campylobacter and Bacillus cereus. The experimental setup is based on a common path interferometer using Mirau interferometric objective lens. White interferograms are recorded 3-chip CCD camera prism technology. have lesser cross talk better spatial resolution comparison to single chip camera. A piezo-electric transducer...