A5071556738- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA Research and Splicing
- Extracellular vesicles in disease
- Advanced biosensing and bioanalysis techniques
- Signaling Pathways in Disease
- Cancer-related Molecular Pathways
- Circular RNAs in diseases
- Neuroblastoma Research and Treatments
- Metastasis and carcinoma case studies
- Genomics and Phylogenetic Studies
- Cancer Cells and Metastasis
- RNA regulation and disease
- Cancer Genomics and Diagnostics
- interferon and immune responses
- Synthetic Organic Chemistry Methods
UiT The Arctic University of Norway
2003-2023
Breast cancer is a heterogeneous disease, and different subtypes of breast show distinct cellular morphology, gene expression, metabolism, motility, proliferation, metastatic potential. Understanding the molecular features responsible for this heterogeneity important correct diagnosis better treatment strategies. Extracellular vesicles (EVs) their associated molecules have gained much attention as players in intercellular communication, ability to precondition specific organs invasion,...
In addition to splicing, group I intron RNA is capable of an alternative two-step processing pathway that results in the formation full-length circular RNA. The circularization initiated by hydrolytic cleavage at 3′ splice site and followed a transesterification reaction which terminal guanosine attacks 5′ presented structure analogous first step splicing. products reactions are unligated exons. For this reason, benefit expense host. has distinct structural requirements differ from those...
MicroRNA profiling represents an important first-step in deducting individual RNA-based regulatory function a cell, tissue, or at specific developmental stage. Currently there are several different platforms to choose from order make the initial miRNA profiles. In this study we investigate recently developed digital microRNA high-throughput technologies. Four were compared including next generation SOLiD ligation sequencing and Illumina HiSeq sequencing, hybridization-based NanoString...
Trans-splicing group I ribozymes have been introduced in order to mediate RNA reprogramming (including repair) of therapeutically relevant transcripts. Efficient depends on the appropriate efficiency reaction, and several attempts, including optimization target recognition ribozyme catalysis, performed. In most studies, Tetrahymena IC1 has applied. Here we investigate potential IE intron ribozymes, derived from myxomycetes Didymium Fuligo, addition ribozyme, for a mutated alpha-mannosidase...
Epithelial-mesenchymal transition (EMT) is a cellular plasticity program critical for embryonic development and tissue regeneration, aberrant EMT associated with disease including cancer. The high degree of in the mammary epithelium reflected extensive heterogeneity among breast cancers. Here, we have analyzed RNA-sequencing data from three different epithelial cell line-derived models identified robust gene expression signature that separates cancers into distinct subgroups. Most...
Group I ribozymes are naturally occurring catalytic RNAs that able to excise themselves as introns (group introns) from a precursor RNA, and ligate the flanking exons. can be engineered act in trans by recognizing separate RNA molecule sequence specific manner, covalently link an this molecule. This ribozyme transesterification reaction has potential molecular biology medicine new approach gene therapy. Here we describe detailed optimized protocols where trans-splicing group applied mapping...