A5062884064- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Parasite Biology and Host Interactions
- Parasitic Diseases Research and Treatment
- Melanoma and MAPK Pathways
- Parasites and Host Interactions
- Toxoplasma gondii Research Studies
- Cancer-related Molecular Pathways
- Virology and Viral Diseases
- Parasitic Infections and Diagnostics
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Studies on Chitinases and Chitosanases
- Synthesis and biological activity
- Biochemical and Molecular Research
- Cardiomyopathy and Myosin Studies
- SARS-CoV-2 and COVID-19 Research
- Calcium signaling and nucleotide metabolism
- Hepatitis B Virus Studies
- Animal Diversity and Health Studies
- Viral gastroenteritis research and epidemiology
- Protein Tyrosine Phosphatases
- bioluminescence and chemiluminescence research
- Animal Virus Infections Studies
Tribhuvan University
2019-2024
UiT The Arctic University of Norway
2017-2021
Institute of Agriculture and Animal Science
2019-2020
Article11 June 2020Open Access Source DataTransparent process CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy Thaddaeus Mutugi Nthiga Department of Medical Biology, Molecular Cancer Research Group, University Tromsø—The Arctic Norway, Tromsø, Norway Search for more papers by this author Birendra Kumar Shrestha orcid.org/0000-0002-8972-0308 Eva Sjøttem Jack-Ansgar Bruun orcid.org/0000-0003-0614-2790 Kenneth Bowitz Larsen orcid.org/0000-0001-6061-4686 Zambarlal Bhujabal Trond...
Abstract p62/SQSTM1 is an autophagy receptor and signaling adaptor with N-terminal PB1 domain that forms the scaffold of phase-separated p62 bodies in cell. The molecular determinants govern filament formation vitro remain to be determined role filaments inside cell currently unclear. We here determine four high-resolution cryo-EM structures different human Arabidopsis assemblies observed a filamentous ultrastructure using correlative cellular EM. show oligomerization or polymerization,...
The cysteine protease ATG4B cleaves off one or more C-terminal residues of the inactive proform proteins ortholog and paralog LC3 GABARAP subfamilies yeast Atg8 to expose a glycine that is conjugated phosphatidylethanolamine during autophagosome formation. We show contains LC3-interacting region (LIR) motif important for efficient binding cleavage proteins. solved crystal structures GABARAPL1-ATG4B LIR complex. Analyses in vitro assays, using specific point mutants, clearly showed binds via...
Mitophagy is the degradation of surplus or damaged mitochondria by autophagy. In addition to programmed and stress-induced mitophagy, basal mitophagy processes exert organelle quality control. Here, we show that sorting assembly machinery (SAM) complex protein SAMM50 interacts directly with ATG8 family proteins p62/SQSTM1 act as a receptor for components SAM mitochondrial contact site cristae organizing system (MICOS) complexes. regulates architecture controlling formation MICOS decisive...
The Golgi complex is essential for the processing, sorting, and trafficking of newly synthesized proteins lipids. turnover regulated to meet different cellular physiological demands. role autophagy in Golgi, however, has not been clarified. Here we show that CALCOCO1 binds Golgi-resident palmitoyltransferase ZDHHC17 facilitate degradation by during starvation. Depletion cells causes expansion accumulation its structural membrane proteins. itself degraded together with other such as TMEM165....
Human ATG8 family proteins (ATG8s) are active in all steps of the macroautophagy pathway, and their lipidation is essential for autophagosome formation. Lipidated ATG8s anchored to outer surface phagophore serve as scaffolds binding other core autophagy various effector involved trafficking or fusion events, whereas those at inner needed assembly selective substrates. Their scaffolding role depends on specific interactions between LC3-interacting region (LIR) docking site (LDS) LIR motifs...
Human ATG8 family proteins (ATG8s) are active in all steps of the macroautophagy pathway, and their lipidation is essential for autophagosome formation. Lipidated ATG8s anchored to outer surface phagophore serve as scaffolds binding other core autophagy various effector involved trafficking or fusion events, whereas those at inner needed assembly selective substrates. Their scaffolding role depends on specific interactions between LC3-interacting region (LIR) docking site (LDS) LIR motifs...
The endoplasmic reticulum (ER) is the largest membrane-bound organelle in eukaryotic cells and plays critical roles diverse processes metabolism, signaling intracellular organization. In response to stress stimuli such as nutrient deprivation, accumulation of misfolded proteins or exposure chemicals, ER increases size through upregulated synthesis its components counteract stress. To restore physiological size, excess are continuously dismantled degraded by reticulophagy, a form autophagy...
Introduction: Dipylidium caninum (double-pored tapeworm) is a common tapeworm found in the small intestines of dogs, cats, foxes, and occasionally humans. It zoonotic parasite that causes dipylidiasis, commonly known as dog tapeworm, which acquired by ingesting infected fleas. The major goal this study to determine prevalence D. stray pet dogs Siddharthanagar municipality, along with its various risk factors (age, sex, breed, deworming status, flea infestation). Methods: This cross-sectional...
Abstract p62/SQSTM1 is an autophagy receptor and signaling adaptor with N-terminal PB1 domain that forms the scaffold of phase-separated p62 bodies in cell. The molecular determinants govern filament formation vitro remain to be determined role filaments inside cell currently unclear. We four high-resolution cryo-EM structures different human Arabidopsis assemblies observed a filamentous ultrastructure using correlative cellular EM. show oligomerization or polymerization, driven by double...