A5054559912- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Cardiomyopathy and Myosin Studies
- Complement system in diseases
- Cellular transport and secretion
- Diabetes Treatment and Management
- Enzyme Catalysis and Immobilization
- Receptor Mechanisms and Signaling
- Platelet Disorders and Treatments
- Cannabis and Cannabinoid Research
- Seed Germination and Physiology
- Plant Molecular Biology Research
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Mycobacterium research and diagnosis
- Renal Diseases and Glomerulopathies
- Bacterial Genetics and Biotechnology
- Adenosine and Purinergic Signaling
- Macrophage Migration Inhibitory Factor
- Coastal and Marine Dynamics
- Drug Transport and Resistance Mechanisms
- Pancreatic function and diabetes
- Autophagy in Disease and Therapy
- Soybean genetics and cultivation
- Genomics and Chromatin Dynamics
European Molecular Biology Laboratory
2020-2023
Institut de Pharmacologie et de Biologie Structurale
2022
Hamburg University of Technology
2022
Universität Hamburg
2022
RELX Group (United States)
2021
European Molecular Biology Laboratory
2019-2020
Ernst Ruska Centre
2019-2020
Forschungszentrum Jülich
2019-2020
University of Regensburg
2011-2019
Delft University of Technology
2019
Transcript elongation factors (TEFs) are a heterogeneous group of proteins that control the efficiency transcript subsets genes by RNA polymerase II (RNAPII) in chromatin context. Using reciprocal tagging combination with affinity purification and mass spectrometry, we demonstrate Arabidopsis thaliana, TEFs SPT4/SPT5, SPT6, FACT, PAF1-C, TFIIS copurified each other elongating RNAPII, while P-TEFb was not among interactors. Additionally, NAP1 histone chaperones, ATP-dependent remodeling...
Abstract p62/SQSTM1 is an autophagy receptor and signaling adaptor with N-terminal PB1 domain that forms the scaffold of phase-separated p62 bodies in cell. The molecular determinants govern filament formation vitro remain to be determined role filaments inside cell currently unclear. We here determine four high-resolution cryo-EM structures different human Arabidopsis assemblies observed a filamentous ultrastructure using correlative cellular EM. show oligomerization or polymerization,...
Significance The complement system is an essential arm within the innate immune defense. Complement contributes to elimination of objects presenting danger signals such as pathogens, dying host cells, and abnormal molecular structures capable inducing inflammatory response stimulating further responses. C1 complex a giant proteolytic enzyme, which plays leading role, because it first component in cascade initiated when activated. On basis structural characterization with X-rays electron...
The structure of the mycobacterial ESX-5 secretion system reveals a central transmembrane pore.
ESCRT-III proteins mediate a range of cellular membrane remodeling activities such as multivesicular body biogenesis, cytokinesis, and viral release. Critical to these processes is the assembly subunits into polymeric structures. In this study, we determined cryo-EM structure helical Saccharomyces cerevisiae Vps24 at 3.2-Å resolution found that adopts an elongated open conformation. forms domain-swapped dimer extended protofilaments associate double-stranded apolar filament. We demonstrate...
Contraction of cortical actomyosin networks driven by myosin activation controls cell shape changes and tissue morphogenesis during animal development. In vitro studies suggest that contractility also depends on the geometrical organization actin filaments. Here we analyze function network topology in vivo using optogenetic stimulation myosin-II Drosophila embryos. We show early cellularization, hexagonally arrayed fibers are resilient to activation. Actomyosin then acquire a ring-like...
TFIIS is a transcript elongation factor that facilitates transcription by RNA polymerase II, as it assists the enzyme to bypass blocks mRNA synthesis. Previously, we have reported Arabidopsis plants lacking exhibit reduced seed dormancy. Among genes differentially expressed in tfIIs seeds, DOG1 gene was identified known QTL for Here analysed overexpress wild type background, or harbour an additional copy of mutant background. These experiments demonstrate down‐regulation expression causes...
TFIIS is a transcript elongation factor that facilitates transcription by RNA polymerase II through blocks to elongation. Arabidopsis plants lacking are affected in seed dormancy, which represents block complete germination under favourable conditions. We have comparatively profiled the levels of seeds tfIIs mutants and control plants. Among differentially expressed genes, DOG1 gene was identified QTL for dormancy. The reduced expression confirmed quantitative RT-PCR Northern analyses,...
Abstract Congenital heart defects are a major cause of perinatal mortality and morbidity, affecting >1% all live births in the Western world, yet large fraction such have an unknown etiology. Recent studies demonstrated surprising dual roles for immune-related molecules their effector mechanisms during fetal development adult homeostasis. In this article, we describe function endogenous complement inhibitor, mannan-binding lectin (MBL)-associated protein (MAp)44, regulating...
Abstract To establish an infection, pathogenic mycobacteria use the Type VII secretion or ESX system to secrete virulence proteins across their cell envelope. The five systems (ESX-1 ESX-5) have evolved diverse functions in cell, with ESX-5 found almost exclusively pathogens. Here we present a high-resolution cryo-electron microscopy structure of hexameric system. This 2.1 MDa membrane protein complex is built by total 30 subunits from six protomeric units, which are composed core components...
The underlying genetic defect in most cases of dilated cardiomyopathy (DCM), a common inherited heart disease, remains unknown. Intriguingly, many patients carry single missense variants uncertain pathogenicity targeting the giant protein titin, fundamental sarcomere component. To explore deleterious potential these variants, we first solved wild-type and mutant crystal structures I21, titin domain targeted by pathogenic variant p.C3575S. Although both are remarkably similar, reduced...
Abstract Thrombospondin type-1 domain-containing 7A (THSD7A) is a large extracellular protein that found in podocyte foot processes of the kidney glomerulus. It has been established as causative autoantigen membranous nephropathy. Amongst predicted 21 thrombospondin repeat domains its segment, highest frequency autoimmune response associated with two N-terminal domains. Here, we show antibodies against this THSD7A segment mice induce typical clinical and morphological signs The...
Abstract Due to their low-lying coastal location, ports are vulnerable climate change induced increases flooding, waves, extreme winds, and the associated costly damages port infrastructure operational disruptions. For these reasons, there is an increasing need for undertake regular risk assessments of vulnerability operations due impacts change. A digital twin, cloud-based modelling solution has been developed enable in-house be undertaken any port. Once set-up, system supports continued...
ABSTRACT The underlying genetic defect in most cases of dilated cardiomyopathy (DCM), a common inherited heart disease, remains unknown. Intriguingly, many patients carry single missense variants uncertain pathogenicity targeting the giant protein titin, fundamental sarcomere component. To explore deleterious potential these variants, we first solved wild-type and mutant crystal structures I21, titin domain targeted by pathogenic variant p.C3575S. Although both are remarkably similar,...