A5003950064- Advanced Electron Microscopy Techniques and Applications
- Cellular transport and secretion
- Quantum, superfluid, helium dynamics
- Bacterial Genetics and Biotechnology
- Lipid Membrane Structure and Behavior
- Advanced Thermodynamics and Statistical Mechanics
- Electron and X-Ray Spectroscopy Techniques
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Nanopore and Nanochannel Transport Studies
- Autophagy in Disease and Therapy
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Bacterial biofilms and quorum sensing
- Atomic and Subatomic Physics Research
- nanoparticles nucleation surface interactions
- Geotechnical Engineering and Soil Stabilization
- Electrochemical Analysis and Applications
- interferon and immune responses
- Methane Hydrates and Related Phenomena
- Mining and Gasification Technologies
- Protein Structure and Dynamics
- Escherichia coli research studies
- Geotechnical Engineering and Analysis
- Legionella and Acanthamoeba research
Delft University of Technology
2019-2025
Heidelberg University
2024
Central Institute of Mental Health
2024
European Molecular Biology Laboratory
2017-2021
Technical University of Munich
2019
Universität der Bundeswehr München
2019
RIKEN Center for Computational Science
2017-2019
Forschungszentrum Jülich
2019
Heinrich Heine University Düsseldorf
2016
Abstract p62/SQSTM1 is an autophagy receptor and signaling adaptor with N-terminal PB1 domain that forms the scaffold of phase-separated p62 bodies in cell. The molecular determinants govern filament formation vitro remain to be determined role filaments inside cell currently unclear. We here determine four high-resolution cryo-EM structures different human Arabidopsis assemblies observed a filamentous ultrastructure using correlative cellular EM. show oligomerization or polymerization,...
Gas vesicles are gas-filled nanocompartments that allow a diverse group of bacteria and archaea to control their buoyancy. The molecular basis properties assembly remains unclear. Here, we report the 3.2 Å cryo-EM structure gas vesicle shell made from structural protein GvpA self-assembles into hollow helical cylinders closed off by cone-shaped tips. Two half shells connect through characteristic arrangement monomers, suggesting mechanism biogenesis. fold features corrugated wall typical for...
ESCRT-III proteins mediate a range of cellular membrane remodeling activities such as multivesicular body biogenesis, cytokinesis, and viral release. Critical to these processes is the assembly subunits into polymeric structures. In this study, we determined cryo-EM structure helical Saccharomyces cerevisiae Vps24 at 3.2-Å resolution found that adopts an elongated open conformation. forms domain-swapped dimer extended protofilaments associate double-stranded apolar filament. We demonstrate...
Abstract Guanylate-binding proteins (GBPs) are interferon-inducible guanosine triphosphate hydrolases (GTPases) mediating host defense against intracellular pathogens. Their antimicrobial activity hinges on their ability to self-associate and coat pathogen-associated compartments or cytosolic bacteria. Coat formation depends GTPase but how nucleotide binding hydrolysis prime remains unclear. Here, we report the cryo-electron microscopy structure of full-length human GBP1 dimer in its guanine...
Cryogenic electron microscopy has become an essential tool for structure determination of biological macromolecules. In practice, the difficulty to reliably prepare samples with uniform ice thickness still represents a barrier routine high-resolution imaging and limits current throughput technique. We show that nanofluidic sample support well-defined geometry can be used cryo-EM specimens reproducible from picoliter volumes. The solution is contained in electron-transparent nanochannels...
Antimicrobial resistance poses a severe threat to human health and Pseudomonas aeruginosa stands out among the pathogens responsible for this emergency. The SOS response DNA damage is crucial in bacterial evolution, influencing development adaptability challenging environments, especially under antibiotic exposure. Recombinase A (RecA) transcriptional repressor LexA are key players that orchestrate process, determining either silencing or active transcription of genes their control. By...
Structure determination of helical specimens commonly requires datasets from thousands micrographs often obtained by automated cryo-EM data acquisition. Interactive tracing assemblies such a number is labor-intense and time-consuming. Here, we introduce an tool MicHelixTrace that precisely locates helix traces rigid as well very flexible with small numbers false positives. The computer program fast has low computational requirements. In addition to coordinates required for subsequent...
Understanding the role of axons in neuronal information processing is a fundamental task neuroscience. Over last years, sophisticated patch-clamp investigations have provided unexpected and exciting data on axonal phenomena functioning, but there still need for methods to investigate full arbors at sufficient throughput. Here, we present new method simultaneous mapping large number individual neurons, which relies their extracellular signals that been recorded with high-density...
Guanylate-Binding Proteins (GBPs) are interferon-inducible guanosine triphosphate hydrolases (GTPases) that mediate immune effector functions against intracellular pathogens. A key step for the antimicrobial activity of GBPs is formation homo- and heterooligomeric complexes on membrane pathogen-associated compartments or cytosolinvasive bacteria. Similar to other large GTPases dynamin family, oligomerisation association depend their GTPase activity. How nucleotide binding hydrolysis prime...
Abstract Antimicrobial resistance represents a major threat to human health and Pseudomonas aeruginosa stands out among the pathogens responsible for this emergency. The SOS response DNA damage plays pivotal role in bacterial evolution, driving development of mechanisms influencing adaptability populations challenging environments, particularly context antibiotic exposure. Recombinase A (RecA) transcriptional repressor LexA are key players that orchestrate process, determining either...
Gas vesicles allow a diverse group of bacteria and archaea to move in the water column by controlling their buoyancy (1). These gas-filled cellular nanocompartments are formed up micrometers long protein shells that permeable only gas. The molecular basis unique properties mechanism assembly remains unknown. Here, we solve 3.2 Å cryo-EM structure B . megaterium gas vesicle shell made from structural GvpA self-assembles into hollow helical cylinders closed off cone-shaped tips. Remarkably,...
Summary PspA is the main effector of phage shock protein (Psp) system and preserves bacterial inner membrane integrity function. Here, we present 3.6 Å resolution cryo-EM structure assembled in helical rods. monomers adopt a canonical ESCRT-III fold an extended open conformation. rods are capable enclosing lipids generate positive curvature. Using visualized how remodels vesicles into μm-sized structures it mediates formation internalized vesicular structures. Hot spots these activities...
Abstract A high-density microelectrode arrays (HDMEA) with 3,150 electrodes per square millimetre was used to capture neuronal activity across various scales, including axons, dendrites, and networks. We present a new method for high-throughput segmentation of axons based on the spatial smoothness signal delays. Comparison both ground truth receiver operator characteristics shows that outperforms previous methods signal-amplitude-to-noise ratio. Structural functional network connectivity...
Abstract Compaction control is a fundamental part of quality assurance for earthworks and essential to ensuring the long‐term stability serviceability earth structures. As testing relative compaction coarse‐grained materials time‐consuming, plate load tests are typically used this purpose. Practical experience shows that secondary using not as straightforward suggested in earthwork specifications. Unfortunately, these subtleties widely known lead rejection compacted layers, despite material...
Abstract p62/SQSTM1 is an autophagy receptor and signaling adaptor with N-terminal PB1 domain that forms the scaffold of phase-separated p62 bodies in cell. The molecular determinants govern filament formation vitro remain to be determined role filaments inside cell currently unclear. We four high-resolution cryo-EM structures different human Arabidopsis assemblies observed a filamentous ultrastructure using correlative cellular EM. show oligomerization or polymerization, driven by double...
ESCRT-III proteins mediate a range of membrane remodeling events such as multivesicular body biogenesis, cytokinesis and viral budding.Little atomic resolution information is available on how monomers come together to facilitate remodeling.In this study we determined the cryo-EM structure filamentous assembly yeast Vps24 at 3.2 Å resolution.We found that adopt an elongated open conformation bind with another monomer form domain-swapped dimer central symmetry axis.The dimers combine apolar...