A5062483864- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Tuberculosis Research and Epidemiology
- Bacterial biofilms and quorum sensing
- Biochemical and Structural Characterization
- RNA and protein synthesis mechanisms
- Pneumocystis jirovecii pneumonia detection and treatment
- Influenza Virus Research Studies
- Phenothiazines and Benzothiazines Synthesis and Activities
- Synthesis and biological activity
- SARS-CoV-2 and COVID-19 Research
- Chemical Synthesis and Analysis
- Escherichia coli research studies
- Plant and fungal interactions
- Adipose Tissue and Metabolism
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Use and Resistance
- Diet and metabolism studies
- Computational Drug Discovery Methods
- Fungal and yeast genetics research
- Monoclonal and Polyclonal Antibodies Research
- Protein Structure and Dynamics
- Mitochondrial Function and Pathology
- Toxic Organic Pollutants Impact
- Enzyme Structure and Function
University of Padua
2020-2025
The current trend dealing with the production of per- and polyfluoroalkyl substances (PFASs) involves shifting toward branched short-chain fluorinated compounds known as new-generation PFASs. A key aspect to be clarified, address adverse health effects associated exposure PFASs, is their binding mode human serum albumin (hSA), most abundant protein in plasma. In this study, we investigated interaction between hSA two representative namely, HPFO-DA C6O4. In-solution studies revealed that both...
Metallo-β-lactamases (MBLs) contribute to the resistance of Gram-negative bacteria carbapenems, last-resort antibiotics at hospital, and MBL inhibitors are urgently needed preserve these important antibacterial drugs. Here, we describe a series 1,2,4-triazole-3-thione-based displaying an α-amino acid substituent, which amine was mono- or disubstituted by (hetero)aryl groups. Compounds certain nitrogen-containing heterocycles showed submicromolar activities against VIM-type enzymes strong...
Antimicrobial resistance poses a severe threat to human health and Pseudomonas aeruginosa stands out among the pathogens responsible for this emergency. The SOS response DNA damage is crucial in bacterial evolution, influencing development adaptability challenging environments, especially under antibiotic exposure. Recombinase A (RecA) transcriptional repressor LexA are key players that orchestrate process, determining either silencing or active transcription of genes their control. By...
The SOS response is among the most conserved pathways that promote acquisition of antibiotic resistance in bacteria. This study aimed to identify and characterize small-molecule inhibitors system opportunistic pathogen Pseudomonas aeruginosa . A library 318 drug-like compounds was screened for inhibition RecA-induced LexA autoproteolysis, a key step activation. One hit compound, 3-(2-sulfanylanilino)propanoic acid, showed dose-dependent with an IC50 mid-micromolar range. Differential...
Photosynthetic organisms use sunlight to produce ATP and NADPH powering their metabolism. Harnessing these products for driving biocatalytic reactions would enable develop clean sustainable alternatives chemical reactions. In this study, we present the first demonstration that produced from photosynthetic process, can fuel a transformation in whole-cell configuration. This result was achieved by expressing cyanobacterium Synechocystis sp. PCC 6803 an ATP-dependent enzyme,...
Aiming at expanding the portfolio of Old Yellow Enzymes (OYEs), which have been systematically studied to be employed in chemical and pharmaceutical industries as useful biocatalysts, we decided explore immense reservoir filamentous fungi. We drew from genome two Ascomycetes Aspergillus niger Botryotinia fuckeliana four new members OYE superfamily belonging classical thermophilic-like subfamilies. The BfOYEs show wider substrate spectra than AnOYE homologues, appear more specialized...
Abstract Macrocyclic peptides provide an attractive modality for drug development due to their ability bind challenging targes, small size, and amenability powerful in vitro evolution techniques such as phage or mRNA display. While these technologies proved capable of generating screening extremely large libraries yielded ligands already many targets, they often do not identify the best binders within a library difficulty monitoring performance controlling selection pressure. Furthermore,...
SARS-CoV-2 is the pathogen responsible for most recent global pandemic, which has claimed hundreds of thousands victims worldwide. Despite remarkable efforts to develop an effective vaccine, concerns have been raised about actual protection against novel variants. Thus, researchers are eager identify alternative strategies fight this pathogen. Like other opportunistic entities, a key step in lifecycle maturation envelope glycoprotein at RARR685↓ motif by cellular enzyme Furin. Inhibition...
The worldwide emergence and dissemination of Gram-negative bacteria expressing metallo-β-lactamases (MBLs) menace the efficacy all β-lactam antibiotics, including carbapenems, a last-line treatment usually restricted to severe pneumonia urinary tract infections. Nonetheless, no MBL inhibitor is yet available in therapy. We previously identified series 1,2,4-triazole-3-thione derivatives acting as micromolar inhibitors MBLs vitro, but devoid synergistic activity microbiological assays. Here,...
Abstract Antimicrobial resistance represents a major threat to human health and Pseudomonas aeruginosa stands out among the pathogens responsible for this emergency. The SOS response DNA damage plays pivotal role in bacterial evolution, driving development of mechanisms influencing adaptability populations challenging environments, particularly context antibiotic exposure. Recombinase A (RecA) transcriptional repressor LexA are key players that orchestrate process, determining either...