A5011151014- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Retinal Development and Disorders
- Developmental Biology and Gene Regulation
- Ubiquitin and proteasome pathways
- Polyomavirus and related diseases
- Osteoarthritis Treatment and Mechanisms
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
- Advanced Proteomics Techniques and Applications
- Ocular Disorders and Treatments
- Streptococcal Infections and Treatments
- Plant Virus Research Studies
- Aquaculture disease management and microbiota
- Calcium signaling and nucleotide metabolism
- CRISPR and Genetic Engineering
- Bacterial Infections and Vaccines
- Cancer-related Molecular Pathways
- Invertebrate Immune Response Mechanisms
- Oral microbiology and periodontitis research
- Studies on Chitinases and Chitosanases
- Blood groups and transfusion
- Complement system in diseases
- Liver physiology and pathology
UiT The Arctic University of Norway
2013-2024
Institute of Medical Biology
2007
Protein degradation by basal constitutive autophagy is important to avoid accumulation of polyubiquitinated protein aggregates and development neurodegenerative diseases. The polyubiquitin-binding p62/SQSTM1 degraded autophagy. It found in cellular inclusion bodies together with proteins cytosolic that accumulate various chronic, toxic, degenerative Here we show for the first time a direct interaction between p62 autophagic effector LC3A -B related gamma-aminobutyrate receptor-associated...
Autophagy is the main eukaryotic degradation pathway for long-lived proteins, protein aggregates, and cytosolic organelles. Although machinery involved in biogenesis of autophagic vesicles well described, very little known about mechanism transport autophagosomes. In this study, we have identified an adaptor complex, formed by two membrane-associated proteins LC3 Rab7 novel FYVE coiled-coil (CC) domain–containing FYCO1, that promotes microtubule (MT) plus end–directed vesicles. We...
It is not clear to what extent starvation-induced autophagy affects the proteome on a global scale and whether it selective. In this study, we report based quantitative proteomics that cells during first 4 h of acute starvation elicit lysosomal degradation up 2–3% proteome. The most significant changes are caused by an immediate autophagic response elicited shortage amino acids but executed independently mechanistic target rapamycin macroautophagy. Intriguingly, receptors p62/SQSTM1, NBR1,...
p62, also known as sequestosome1 (SQSTM1), A170, or ZIP, is a multifunctional protein implicated in several signal transduction pathways. p62 induced by various forms of cellular stress, degraded autophagy, and acts cargo receptor for autophagic degradation ubiquitinated targets. It suggested to shuttle proteins proteasomal degradation. commonly found cytosolic inclusions patients with aggregopathies, it up-regulated human tumors, mutations the gene are linked classical adult onset Paget...
Article11 June 2020Open Access Source DataTransparent process CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy Thaddaeus Mutugi Nthiga Department of Medical Biology, Molecular Cancer Research Group, University Tromsø—The Arctic Norway, Tromsø, Norway Search for more papers by this author Birendra Kumar Shrestha orcid.org/0000-0002-8972-0308 Eva Sjøttem Jack-Ansgar Bruun orcid.org/0000-0003-0614-2790 Kenneth Bowitz Larsen orcid.org/0000-0001-6061-4686 Zambarlal Bhujabal Trond...
The Golgi complex is essential for the processing, sorting, and trafficking of newly synthesized proteins lipids. turnover regulated to meet different cellular physiological demands. role autophagy in Golgi, however, has not been clarified. Here we show that CALCOCO1 binds Golgi-resident palmitoyltransferase ZDHHC17 facilitate degradation by during starvation. Depletion cells causes expansion accumulation its structural membrane proteins. itself degraded together with other such as TMEM165....
Accumulating evidence supports the notion that high-dose (>5 Gy) radiotherapy (RT) regimens are triggering stronger pro-immunogenic effects than standard low-dose (2 regimens. However, of RT on certain immunoregulatory elements in tumors remain unexplored. In this study, we have investigated (HD-RT) immunomodulating functions cancer-associated fibroblasts (CAFs). Primary CAF cultures were established from lung cancer specimens derived patients diagnosed for non-small cell cancer. Irradiated...
This study was undertaken to compare the phenotype of human articular chondrocytes (ACs) and bone marrow-derived mesenchymal stem cells (MSCs) after cell expansion by studying spectrum proteins secreted into culture medium. ACs MSCs were expanded in monolayer cultures for some weeks, as done standard transplantation procedures. Initially, expression cartilage signature genes compared real-time PCR. Metabolic labeling (SILAC) combination with mass spectrometry (LC/MS-MS) applied investigate...
The transcription factor Pax6 is required for normal development of the central nervous system, eyes, nose, and pancreas. Here we show that transactivation domain (TAD) zebrafish phosphorylated in vitro by mitogen-activated protein kinases (MAPKs) extracellular-signal regulated kinase (ERK) p38 but not Jun N-terminal (JNK). Three four putative proline-dependent phosphorylation sites are vitro. Of these sites, serine 413 (Ser413) evolutionary conserved from sea urchin to man. Ser413 also...
The Pax6 genes encode evolutionary conserved transcription factors that act high up in the regulatory hierarchy controlling development of central organs such as eyes and nervous system. These proteins contain two DNA-binding domains. N-terminal paired domain is separated from a paired-type homeodomain by linker region, transactivation located C-terminal to homeodomain. Vertebrate express paired-less isoform (Pax6ΔPD) an internal start codon coding region between We now provide evidence for...
In the context of radiotherapy, collateral effects ablative doses ionizing radiation (AIR) on stromal components tumors remains understudied. this work, cancer-associated fibroblasts (CAFs) isolated from freshly resected human lung were exposed to AIR (1x 18 Gy) and analyzed for their release paracrine factors. Inflammatory mediators regulators angiogenesis tumor growth by multiplex protein assays in conditioned medium (CM) irradiated non-irradiated CAFs. Additionally, profile secreted...
Pseudomonas aeruginosa (PA) CbpD belongs to the lytic polysaccharide monooxygenases (LPMOs), a family of enzymes that cleave chitin or related polysaccharides. Here, we demonstrate virulence role in PA pneumonia linked impairment host complement function and opsonophagocytic clearance. Following intratracheal challenge, ΔCbpD mutant was more easily cleared produced less mortality than wild-type parent strain. The x-ray crystal structure LPMO domain solved subatomic resolution (0.75Å) its two...
Abstract The main goal of our study was to analyze and compare the profiles secreted proteins from adult human articular chondrocytes in monolayers, cartilage explants culture, using a de novo protein labeling approach. Stable isotope culture used differentiate between chondrocyte‐derived other preexisting matrix‐derived components, or coming serum synovial fluids. Proteins supernatants were resolved by one‐dimensional SDS‐PAGE electrophoresis, analyzed tandem with LC/MS‐MS (liquid...
Fish immunology research is at a pivotal point with the increasing availability of functional immunoassays and major advances in -omics approaches. However, studies on fish B cells their distinct subsets remain challenge due to limited differentially expressed surface markers. To address this constraint, cell proteome Atlantic salmon IgM+ were analyzed by mass spectrometry compared proteins detected from two adherent head kidney lines, ASK SSP-9. Out 21 cluster differentiation (CD) molecules...
Autophagy is a highly conserved degradative pathway, essential for cellular homeostasis and implicated in diseases including cancer neurodegeneration. Autophagy-related 8 (ATG8) proteins play central role autophagosome formation selective delivery of cytoplasmic cargo to lysosomes by recruiting autophagy adaptors receptors. The LC3-interacting region (LIR) docking site (LDS) ATG8 binds LIR motifs present LIR-ATG8 interactions can be specific mammalian family members (LC3A-C, GABARAP,...
Extracellular vesicles or exosomes constitute an evolutionarily conserved mechanism of intercellular signaling. Exosomes are gaining increasing amount attention due to their role in pathologies, including malignancy, importance as prognostic and diagnostic markers, potential a therapeutic tool. Merkel cell carcinoma (MCC) is aggressive form skin cancer with poor prognosis. Because effective systemic treatment for this type currently not available, exosome-based therapy was proposed. However,...
Human ATG8 family proteins (ATG8s) are active in all steps of the macroautophagy pathway, and their lipidation is essential for autophagosome formation. Lipidated ATG8s anchored to outer surface phagophore serve as scaffolds binding other core autophagy various effector involved trafficking or fusion events, whereas those at inner needed assembly selective substrates. Their scaffolding role depends on specific interactions between LC3-interacting region (LIR) docking site (LDS) LIR motifs...
Human ATG8 family proteins (ATG8s) are active in all steps of the macroautophagy pathway, and their lipidation is essential for autophagosome formation. Lipidated ATG8s anchored to outer surface phagophore serve as scaffolds binding other core autophagy various effector involved trafficking or fusion events, whereas those at inner needed assembly selective substrates. Their scaffolding role depends on specific interactions between LC3-interacting region (LIR) docking site (LDS) LIR motifs...
The transcription factor Pax6 is essential for the development of eyes and central nervous system vertebrates invertebrates. contains two DNA-binding domains; an N-terminal paired domain a centrally located homeodomain. We have previously shown that vertebrate paired-less isoform (Pax6ΔPD), several other homeodomain proteins, interact with full-length enhancing Pax6-mediated transactivation from domain-DNA binding sites. By mutation analyses molecular modeling we now demonstrate that,...
ABSTRACT The minor capsid protein of human BK polyomavirus (BKPyV), VP2, and its N-terminally truncated form, VP3, are both important for viral entry. closely related simian virus 40 (SV40) reportedly produces an additional form VP2/3, denoted VP4, apparently functioning as a viroporin promoting progeny release. VP4 open reading frame is conserved in some polyomaviruses, including BKPyV. In this study, we investigated the role BKPyV replication. By transfecting genomes into primary renal...