A5014246009- Complement system in diseases
- Blood Coagulation and Thrombosis Mechanisms
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Porphyrin Metabolism and Disorders
- Blood groups and transfusion
- Heme Oxygenase-1 and Carbon Monoxide
- Renal Transplantation Outcomes and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Atherosclerosis and Cardiovascular Diseases
- Venous Thromboembolism Diagnosis and Management
- Bacterial biofilms and quorum sensing
- Neonatal Health and Biochemistry
- Lipoproteins and Cardiovascular Health
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Respiratory Support and Mechanisms
- Renal Diseases and Glomerulopathies
- Organ Transplantation Techniques and Outcomes
- Fatty Acid Research and Health
- Mosquito-borne diseases and control
- Systemic Lupus Erythematosus Research
- Neurological Complications and Syndromes
- Methemoglobinemia and Tumor Lysis Syndrome
- Antibiotic Resistance in Bacteria
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Nordland Hospital
2014-2024
Nordland Hospital Trust
2018-2023
UiT The Arctic University of Norway
2020
The recently discovered lytic polysaccharide monooxygenases (LPMOs), which cleave polysaccharides by oxidation, have been associated with bacterial virulence, but supporting functional data is scarce. Here we show that CbpD, the LPMO of Pseudomonas aeruginosa, a chitin-oxidizing virulence factor promotes survival bacterium in human blood. catalytic activity CbpD was promoted azurin and pyocyanin, two redox-active factors also secreted P. aeruginosa. Homology modeling, molecular dynamics...
Staphylococcus aureus produces membrane-derived vesicles (MVs), which share functional properties to outer membrane (OMVs). Atomic force microscopy (AFM) revealed that S. aureus-derived MVs are associated with the bacterial surface or released into surrounding environment depending on growth conditions. By using a comparative proteomic approach, total of 131 and 617 proteins were identified in isolated from grown Luria-Bertani (LB) Brain-Heart infusion (BHI) broth, respectively. Purified...
Pseudomonas aeruginosa (PA) CbpD belongs to the lytic polysaccharide monooxygenases (LPMOs), a family of enzymes that cleave chitin or related polysaccharides. Here, we demonstrate virulence role in PA pneumonia linked impairment host complement function and opsonophagocytic clearance. Following intratracheal challenge, ΔCbpD mutant was more easily cleared produced less mortality than wild-type parent strain. The x-ray crystal structure LPMO domain solved subatomic resolution (0.75Å) its two...
Abstract CD14 is a key recognition molecule of innate immune responses, interacting with several TLRs. TLR signaling cross-talks extensively the complement system, and combined inhibition has been proved effective in attenuating inflammatory responses. Pig models human diseases have emerged as valuable tools to study therapeutic intervention, but suitable neutralizing Abs are rare. Undesired Fc-mediated functions, such platelet activation IL-8 release induced by porcine CD14-specific clone...
This study aimed to examine whether acute intermittent porphyria (AIP) is associated with systemic inflammation and the correlates disease activity. A case-control 50 AIP cases age-, sex- place of residence-matched controls was performed. Plasma cytokines, insulin C-peptide were analysed after an overnight fast using multiplex assay. Long pentraxin-3 (PTX3) complement activation products (C3bc TCC) enzyme-linked immunosorbent assay (ELISA). Urine porphobilinogen ratio (U-PBG, µmol/mmol...
Essentials Complement, Toll-like receptors and coagulation cross-talk in the process of thromboinflammation. This is explored a unique human whole-blood model S. aureus bacteremia. Coagulation here shown as downstream event C5a-induced tissue factor (TF) production. Combined inhibition C5 CD14 efficiently attenuated TF coagulation.Background There extensive between complement system, (TLRs), hemostasis. Consumptive coagulopathy hallmark sepsis, often mediated through increased expression....
Lifestyle factors, including a low intake of carbohydrates, dieting, alcohol consumption, cigarette smoking and stress are some the possible triggers attacks in acute intermittent porphyria (AIP). The influence lifestyle energy intake, diet consumption on biochemical disease activity AIP nutritional markers were examined.A case-control study with 50 cases controls matched for age, sex place residence was performed. Dietary registered using food diary 46 pairs. Symptoms, other triggering...
Both the complement system and tissue factor (TF), a key initiating component of coagulation, are activated in sepsis, cross-talk occurs between coagulation systems. C1-inhibitor (C1-INH) can act as regulator both Our aim this study was to examine by investigating effects C1-INH on Escherichia coli-induced haemostasis inflammation. Fresh human whole blood collected lepirudin incubated with E. coli or ultrapurified lipopolysaccharide (LPS) absence presence protease-inactivated C1-INH. C3...
Air embolism may complicate invasive medical procedures. Bubbles trigger complement C3-mediated cytokine release, coagulation, and platelet activation in vitro human whole blood. Since these findings have not been verified vivo, we aimed to examine the effects of air pigs on thromboinflammation.Forty-five landrace pigs, average 17 kg (range 8.5-30), underwent intravenous infusion for 300 or 360 minutes (n=29) served as sham (n=14). Fourteen were excluded due e.g. infections persistent...
Morbidly obese patients are at risk of developing insulin resistance and cardiovascular disease. Low-grade systemic inflammation is an important factor for this development. We evaluated the effect bariatric surgery on markers inflammation, coagulation glucose metabolism.Ninety-seven morbidly 17 lean subjects (control group) participated. Anthropometric measurements as well fasting blood samples were obtained first admission, prior to surgery, 1 year after surgery.At group had significantly...
Background: It has been proposed that irritable bowel syndrome (IBS) is a low-grade mucosal inflammatory disease.Objective: To characterize the intestinal profile in IBS patients with or without fructose intolerance.Design: Patients referred to colonoscopy complaints were screened for participation. diagnosed according Rome II criteria and no organic gastrointestinal disease included study. One subgroup was fructose-reduced diet study 2 months effects based on VAS symptom scores. Healthy...
Abstract Venous air embolism, which may complicate medical and surgical procedures, activates complement triggers thromboinflammation. In lepirudin-anticoagulated human whole blood, we examined the effect of bubbles on its role in Whole blood from 16 donors was incubated with without or inhibitors C3, C5, C5aR1, CD14. Complement activation, hemostasis, cytokine release were measured using ELISA quantitative PCR. Compared no air, incubating increased, average, C3a 6.5-fold, C3bc 6-fold,...
Pancreas transplant alone (PTA) recipients are more affected by pancreas graft thrombosis, and loss compared to simultaneous pancreas-kidney (SPK) recipients. The pathophysiology is unknown, but an increased immune response has been suggested in the PTA In this observational study, we perioperative thromboinflammation between (n=32) SPK (n=35) recipients, with (n=14) versus without (n=18) early thrombosis.We measured C-reactive protein (CRP), plasma markers of activated coagulation...
Chronic fatigue syndrome (CFS) is a prevalent and disabling condition among adolescent. The disease mechanisms are unknown. Previous studies have suggested elevated plasma levels of several cytokines, but recent meta-analysis 38 articles found that 77 different cytokines measured in plasma, transforming growth factor beta (TGF-β) was the only one patients compared to controls sufficient number articles. In present study we therefore three TGF-β isoforms adolescent CFS healthy controls....
Ischemic injury worsens upon return of blood and innate immunity including the complement system play a central role in ischemia-reperfusion (IRI) as thoracic aortic surgery. Complement component1 inhibitor (C1-INH) has been shown to reduce IRI is broad-acting plasma cascade inhibitor. We established new porcine model by cross-clamping aorta evaluated global changes occurring organ function, systemic inflammatory response damage with or without treatment C1-INH-concentrate. Twenty-four...
C1-Inhibitor (C1-INH) is an important biological inhibitor, regulating several protein cascade systems. Recent research has shown that the molecule exhibits properties not dependent on its protease inhibition activity. Serum and whole blood from pigs humans were pre-incubated with C1-INH, iC1-INH or complement inhibitors SPICE compstatin. Whole, live Escherichia coli then added for further incubation. Complement activation, a range of cytokines, chemokines growth factors, as well leukocyte...