A5082806905- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Immune Response and Inflammation
- Signaling Pathways in Disease
- RNA and protein synthesis mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Enzyme Production and Characterization
- Cardiac and Coronary Surgery Techniques
- Organ Transplantation Techniques and Outcomes
- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- RNA Research and Splicing
- Blood Coagulation and Thrombosis Mechanisms
- Antimicrobial Peptides and Activities
- Biochemical and Molecular Research
- Bone Metabolism and Diseases
- Cardiac Ischemia and Reperfusion
- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- Immune Cell Function and Interaction
- Bone and Dental Protein Studies
- TGF-β signaling in diseases
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
University of Oslo
2012-2024
Discovery Institute
2016-2017
Sanford Burnham Prebys Medical Discovery Institute
2016-2017
Blindeninstitutsstiftung
2001
Vollum Institute
1994
Heidelberg University
1994
University of California, San Diego
1994
Norwegian Cancer Society
1993
University College London
1993
National Hospital for Neurology and Neurosurgery
1993
ABSTRACT Invasive fungal infections represent an increasing problem associated with high mortality. The present study was undertaken to identify leukocyte subsets that are activated by hyphal fragments in a whole-human-blood model, as well examine the involvement of CD14 and Toll-like receptors (TLRs) activation monocytes hyphae. Incubation whole human blood from Aspergillus fumigatus Scedosporium prolificans for 6 h caused induction mRNAs tumor necrosis factor alpha (TNF-α), interleukin-1β...
We have examined the ability of peptidoglycan (PepG) and lipoteichoic acid (LTA) isolated from Staphylococcus aureus to induce release tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), IL-10 in whole human blood identified cellular origins these cytokines. Both PepG LTA induced transient increases TNF-alpha plasma, with peak values at 6 12 h, respectively. IL-6 increased throughout experimental period (24 h). The TNF-alpha, IL-6, by was dose dependent. Only a potent inducer...
The cysteine protease legumain is involved in several biological and pathological processes, the has been found over-expressed associated with an invasive metastatic phenotype a number of solid tumors. Consequently, proposed as prognostic marker for certain cancers, potential therapeutic target. Nevertheless, details on how advances malignant progression along regulation its proteolytic activity are unclear. In present work, expression was examined colorectal cancer cell lines. Substantial...
W e report that a human neoplastic B cell line (Reh) contains CAMP-dependent protein kinase (cAK) type I (cAKI), but is practically devoid of cAK I1 (cAKII).However, these cells contain novel cAKI isozyme consisting an RIa-RIP heterodimer in association with phosphotransferase activity (RIaRIPC,) eluting from DW-cellulose columns at salt concentration characteristic cAKII. Immunoprecipitation 8-a~ido-[~~P]cAMP-labeled extracts and DEAE fractions employing specific antibodies directed against...
Abstract Background High activity of cysteine proteases such as legumain and the cathepsins have been shown to facilitate growth invasion a variety tumor types. In breast cancer, several recent studies indicated that loss protease inhibitor cystatin E/M leads increased metastasis. Although is normally expressed in skin, its role regulation progression malignant melanoma has not studied. Methods A panel various non-melanoma cell lines was used. Cystatin C were analyzed media by immunoblotting...
The present study was conducted to evaluate the effect of hyperglycaemia in itself on glucose and lipid metabolism human skeletal muscle cells. Satellite cells were isolated from biopsy samples vastus lateralis differentiated into multinucleated myotubes cultures. Metabolism studies performed using isotopes ([3H]deoxyglucose, [14C]glucose, [14C]oleic acid [14C]palmitic acid), mRNA protein levels analysed by real-time RT-PCR western blotting respectively. Exposure 20 mmol/l for 4 days reduced...
Abstract Macrophages express several lysosomal cysteine proteases such as cathepsins and legumain. In this study, we assessed the expression, activity secretion of legumain in cellular models monocytes/macrophages. were derived from M-CSF- or GM-CSF/IFNγ-stimulated human primary monocytes (M2 M1, respectively), PMA-treated THP-1 cells, murine RAW264.7 macrophages. both monocyte-to-macrophage differentiation caused highly increased expression Also, macrophages, but not monocytes, was...
The pathophysiological mechanisms involved in mixed bacterial infections caused by gram-positive and gram-negative bacteria are largely unknown. present study examines the potential interaction between lipopolysaccharide (LPS) peptidoglycan (PepG) induction of sepsis-associated cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-10 whole human blood. Plasma values these were measured enzyme immunoassays a TNF bioassay. Co-administration PepG (10 μg/mL) or muramyl dipeptide...
The present study reports the exon-intron organization of human RIα gene cAMP-dependent protein kinase and approximately 2 kilobases (kb) 5′-flanking region obtained by isolation sequencing several phage clones from genomic libraries. is composed nine coding exons varying lengths, separated introns, giving a total length at least 21 kb. Our recent cloning processed pseudogene with 5′-noncoding different previously reported complementary DNA indicated that may have multiple leader rise to...
Abstract We found leptin to be strongly expressed in undifferentiated human myoblasts derived from biopsies of the thigh (Musculus vastus lateralis). Both mRNA expression and secretion were reduced during vitro differentiation into primary myotubes. However, receptor (OB‐Rb) mRNA, was unchanged muscle cells. Administration recombinant had no effect on leptin, myogenin, myoD, or GLUT4 expressions period cellular differentiation. A functional demonstrated by an acute leptin‐induced 1.5‐fold...
Simvastatin, a HMG-CoA reductase inhibitor, is prescribed worldwide to patients with hypercholesterolemia. Although simvastatin well tolerated, side effects like myotoxicity are reported. The mechanism for statin-induced still poorly understood. Reports have suggested impaired mitochondrial dysfunction as contributor the observed myotoxicity. In this regard, we wanted study of on glucose metabolism and activity legumain, cysteine protease. Legumain, being only known asparaginyl...
The cysteine protease legumain is involved in several biological and pathological processes, the has been found over-expressed associated with an invasive metastatic phenotype a number of solid tumors.Consequently, proposed as prognostic marker for certain cancers, potential therapeutic target.Nevertheless, details on how advances malignant progression along regulation its proteolytic activity are unclear.In present work, expression was examined colorectal cancer cell lines.Substantial...