A5103760823- Access Control and Trust
- FinTech, Crowdfunding, Digital Finance
- Sharing Economy and Platforms
- Organic and Molecular Conductors Research
- Magnetism in coordination complexes
- Blockchain Technology Applications and Security
- Information and Cyber Security
- Data Quality and Management
- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Protein Structure and Dynamics
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Service-Oriented Architecture and Web Services
- Electron and X-Ray Spectroscopy Techniques
- Spam and Phishing Detection
- Privacy-Preserving Technologies in Data
- Advanced Vision and Imaging
- Cloud Data Security Solutions
- Carbon and Quantum Dots Applications
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- Protein Interaction Studies and Fluorescence Analysis
- Silicon Nanostructures and Photoluminescence
- Digital Marketing and Social Media
- Internet Traffic Analysis and Secure E-voting
Bayer (Germany)
2024
Goethe University Frankfurt
2012-2020
University of Kassel
2012-2020
University of Regensburg
2014-2018
University of Bonn
2018
Fraunhofer Institute for High-Speed Dynamics, Ernst-Mach-Institut
2017
Technical University of Munich
2016
Bavarian Academy of Sciences and Humanities
2016
Friedrich-Alexander-Universität Erlangen-Nürnberg
2000-2011
University of Bayreuth
2010
Correlative microscopy incorporates the specificity of fluorescent protein labeling into high-resolution electron micrographs. Several approaches exist for correlative microscopy, most which have used green (GFP) as label light microscopy. Here we use chemical tagging and synthetic fluorophores instead, in order to achieve protein-specific labeling, perform multicolor imaging. We show that preserve their post-embedding fluorescence presence uranyl acetate. Post-embedding is such quality...
Desmosomes are cell-cell junctions that link tissue cells experiencing intense mechanical stress. Although the structure of desmosomal cadherins is known, desmosome architecture-which essential for mediating numerous functions-remains elusive. Here, we recorded cryo-electron tomograms (cryo-ET) in which individual can be discerned; they appear variable shape, spacing, and tilt with respect to membrane. The resulting sub-tomogram average reaches a resolution ∼26 Å, limited by inherent...
Mid-infrared spectroscopy is an essential analytical method in science and industry. Unlike the near-infrared range, grating spectrometers for mid-infrared are rarely employed, mostly due to limited availability performance of suitable line array detectors. In this work, continuous-wave nonlinear-optical upconversion used enable spectroscopy. A broad spectral window between 3.7 4.7 μm upconverted 825 - 867 nm detection on a silicon-camera-based spectrometer with high sensitivity down...
Mycoplasma genitalium, the causative agent of non-gonococcal urethritis and pelvic inflammatory disease in humans, is a small eubacterium that lacks peptidoglycan cell wall. On surface its plasma membrane major adhesion complex, known as NAP essential for gliding motility organism. Here, we have performed cryo-electron tomography intact cells detergent permeabilized M. genitalium aggregates, providing sub-tomogram averages free cell-attached NAPs respectively, revealing tetrameric complex...
Optical flow estimation of image sequences is one the key elements for motion detection. However, processing optical in real-time still an open task due to its computationally expensive nature. In this paper we present FPGA-optimized architecture based on algorithm Horn and Schunck. While existing FPGA-realizations are only partly capable, a Stratix IV our enables computation each pixel frame with 640 × 512 pixels at framerate 30 fps iterative up 4k resolution (4,096 2,304 pixels) 20...
Water-soluble 2- and 4-vinylpyridine terminated silicon quantum dots (SiQDs) with sizes between 2 3 nm were obtained by HF etching of oxidized SiQDs subsequently passivating vinylpyridine ligands via thermal hydrosilylation. The functionalized emit photoluminescence in the blue-green spectrum yields around 30%. is ascribed to radiative recombination excitons confined SiQD core. Indication efficient electronic interactions excited ligand states conduction band has been achieved real-time...
Due to compliance and IT security requirements, company-wide identity access management within organizations has gained significant importance in research practice over the last years. Companies aim at standardizing user policies order reduce administrative overhead strengthen security. These provide foundation for every system no matter if poured into systems or only located responsible (IAM) engineers’ mind. Despite its relevance, hardly any supportive means automated detection refinement...
In theory, silicon quantum dots (SiQDs) emit enhanced photoluminescence with a size-tunable spectrum in the visible range. practice, surface states originating from oxide defect structures or organic ligands are strongly involved exciton relaxation dynamics because amplitudes of hole and electron wave functions nonzero at SiQD surface. this study, SiQDs well-defined properties were obtained through wet-chemistry procedure providing adjustable sizes oxide-free, 3-vinylthiophene-terminated...
We analysed the conformational states of free, tet operator‐bound and anhydrotetracycline‐bound Tet repressor employing a Trp‐scanning approach. The two wild‐type Trp residues in were replaced by Tyr or Phe single introduced at each positions 162–173, representing part an unstructured loop N‐terminal six α‐helix 9. All mutants retained vivo inducibility, but anhydrotetracycline‐binding constants decreased up to 7.5‐fold when was 169, 170 173. Helical (168–173) differed from those (162–167)...
It is demonstrated that backbone chloro(phenyl)phosphanyl‐substituted imidazole‐2‐thiones 3 ( N‐ R = Me, Et, n Bu) can be easily synthesized which allow for a selective access to tricyclic 1,4‐diphenyl‐1,4‐dihydro‐1,4‐diphosphinines 5 via reaction with LDA. Furthermore, new examples preferred substitution reactions are provided occurred when KHMDS was used thus yielding P– N(SiMe ) 2 substituted derivatives 4 . The Ph 1,4‐dihydro‐1,4‐diphosphinine were successfully employed generate...
Due to compliance and IT security requirements, company-wide Identity Access Management within organizations has gained significant importance in research practice over the last years. Companies aim at standardizing user management policies order reduce administrative overhead strengthen security. Despite of its relevance, hardly any supportive means for automated detection refinement as well are available. As a result, outdate time, leading vulnerabilities inefficiencies. Existing mainly...
Recent data breaches caused by highly-privileged insiders (e.g. the NSA/Snowden case) as well proliferation of mobile and cloud applications in enterprises imposes new challenges for Identity Management. To cope with these challenges, business analysts have predicted a variety trends enterprise In this paper, we conduct thorough literature analysis to examine which extent scientific community seizes upon identify major research areas therein. Results show that despite analysts' predictions,...