Brandon J. DeKosky
A5066191092- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Respiratory viral infections research
- Influenza Virus Research Studies
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- SARS-CoV-2 detection and testing
- Advanced Biosensing Techniques and Applications
- Diabetes and associated disorders
- Animal Virus Infections Studies
- Single-cell and spatial transcriptomics
- Virus-based gene therapy research
- COVID-19 Clinical Research Studies
- Cytomegalovirus and herpesvirus research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Virology and Viral Diseases
- Bacillus and Francisella bacterial research
- Viral Infections and Vectors
University of Kansas
2010-2025
Massachusetts Institute of Technology
2021-2025
Ragon Institute of MGH, MIT and Harvard
2021-2025
Massachusetts General Hospital
2024-2025
National Institutes of Health
2015-2020
National Institute of Allergy and Infectious Diseases
2015-2020
Institut de Recherche Vaccinale
2018
The University of Texas at Austin
2013-2017
Significance Most vaccines confer immunity by eliciting long-term production of antibodies that bind to and neutralize the vaccine antigen. Remarkably, very little is known regarding identities, sequence diversity, relative concentrations, or binding functionalities mAbs comprise serum repertoire elicited vaccination. Here, we have delineated constituent human IgG after vaccination examined their relationship antibody V gene encoded circulating B cells. The results detail molecular...
A distinct population of B cells that respond to vaccination serve as potential plasma cell precursors.
Significance We applied a very recently developed experimental strategy for high-throughput sequencing of paired antibody heavy and light chains along with large-scale computational structural modeling to delineate features the human repertoire at unprecedented scale. Comparison repertoires encoded by peripheral naive memory B cells revealed ( i ) preferential enrichment or depletion specific germline gene combinations heavy- light-chain variable regions ii enhanced positive charges, higher...
A new method for encapsulating cells in interpenetrating network (IPN) hydrogels of superior mechanical integrity was developed. In this study, two biocompatible materials-agarose and poly(ethylene glycol) (PEG) diacrylate-were combined to create a IPN hydrogel with greatly enhanced performance. Unconfined compression samples revealed that the displayed fourfold increase shear modulus relative pure PEG-diacrylate (39.9 vs. 9.9 kPa) 4.9-fold agarose (8.2 kPa). PEG compressive failure strains...
Understanding mechanisms of protective antibody recognition can inform vaccine and therapeutic strategies against SARS-CoV-2. We report a monoclonal antibody, 910-30, targeting the SARS-CoV-2 receptor-binding site for ACE2 as member public response encoded by IGHV3-53/IGHV3-66 genes. Sequence structural analyses 910-30 related antibodies explore how class features correlate with neutralization. Cryo-EM structures bound to spike trimer reveal binding interactions its ability disassemble...
Biotin-labeled molecular probes, comprising specific regions of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike, would be helpful in isolation and characterization antibodies targeting this recently emerged pathogen. Here, we design constructs incorporating an N-terminal purification tag, a site-specific protease-cleavage site, probe region interest, C-terminal sequence targeted by biotin ligase. Probe include full-length spike ectodomain as well various subregions,...
Abstract The ongoing evolution of Ebolaviruses poses significant challenges to the development immunodiagnostics for detecting emergent viral variants. There is a critical need discovery monoclonal antibodies with distinct affinities and specificities different Ebolaviruses. We developed an efficient technology rapid plethora antigen-specific from immunized animals by mining V H :V L paired antibody repertoire encoded highly expanded B cells in draining popliteal lymph node (PLN). This...
Since the outbreak of COVID-19 pandemic, widespread infections have allowed SARS-CoV-2 to evolve in human, leading emergence multiple circulating variants. Some these variants show increased resistance vaccine-elicited immunity, convalescent plasma, or monoclonal antibodies. In particular, mutations spike drawn attention. To facilitate isolation neutralizing antibodies and monitoring vaccine effectiveness against variants, we designed produced biotin-labeled molecular probes variant spikes...
Gene syntax—the order and arrangement of genes their regulatory elements—shapes the dynamic coordination both natural synthetic gene circuits. Transcription at one locus profoundly impacts transcription nearby adjacent genes, but molecular basis this effect remains poorly understood. Here, using integrated reporter circuits in human cells, we show that supercoiling-mediated feedback regulates expression a syntax-specific manner. Using Region Capture Micro-C, measure induction-dependent...
Abstract Neutralizing antibodies provide rapid immune defense against infectious diseases, but are difficult to discover at scale because neutralization assays require live reporter cells and soluble monoclonal antibodies. Here we report Droplet Reporter Cell Testing for Neutralization (DrReCT-Neutralization) screen antibody gene libraries their ability neutralize viral infections. We established the necessary engineered cell lines validated DrReCT screening platform using synthetic...