A5010142228- Viral gastroenteritis research and epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Cervical Cancer and HPV Research
- HIV Research and Treatment
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Hepatitis B Virus Studies
- vaccines and immunoinformatics approaches
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Herpesvirus Infections and Treatments
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- Chronic Myeloid Leukemia Treatments
- Influenza Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- PI3K/AKT/mTOR signaling in cancer
- Ubiquitin and proteasome pathways
- HIV/AIDS drug development and treatment
- Chronic Lymphocytic Leukemia Research
- Cancer Research and Treatments
Xiamen University
2016-2025
Stony Brook School
2024-2025
Stony Brook University
2001-2025
National Vaccine and Serum Institute
2025
Second Affiliated Hospital of Zhejiang University
2009-2024
Zhejiang University
1992-2024
MRC Laboratory of Molecular Biology
2024
First Affiliated Hospital Zhejiang University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Children's Hospital of Zhejiang University
2024
Abstract Cyclin-dependent kinase 2 (CDK2) complex is significantly over-activated in many cancers. While it makes CDK2 an attractive target for cancer therapy, most inhibitors against are ATP competitors that either nonspecific or highly toxic, and typically fail clinical trials. One alternative approach to develop non-ATP competitive inhibitors; they disrupt interactions between its partners substrates, resulting specific inhibition of activities. In this report, we identify two potential...
The plant-specific Rop family GTPases are versatile molecular switches in many processes during plant growth, development, and responses to the environment. To understand how achieves its functional versatility signaling, we performed a genome-wide identification of putative targets using combination yeast two-hybrid method, bioinformatic tools, robust assay pollen. In this study, have identified 11 Arabidopsis genes encoding novel proteins, termed RICs (for Rop-interactive CRIB...
Hepatitis E virus (HEV) causes acute hepatitis in humans, predominantly by contamination of food and water, is characterized jaundice flu-like aches pains. To date, no vaccines are commercially available to prevent the disease caused HEV. Previously, we showed that a monoclonal antibody, 8C11, specifically recognizes neutralizing conformational epitope on HEV genotype I. The antibody 8C11 blocks virus-like particle from binding penetrating host cell. Here, report complex crystal structure...
The emergence of numerous variants SARS-CoV-2, the causative agent COVID-19, has presented new challenges to global efforts control COVID-19 pandemic. Here, we obtain two cross-neutralizing antibodies (7D6 and 6D6) that target Sarbecoviruses' receptor-binding domain (RBD) with sub-picomolar affinities potently neutralize authentic SARS-CoV-2. Crystal structures show both bind a cryptic site different from recognized by existing highly conserved across Sarbecovirus isolates. Binding these RBD...
Digital capability is an advanced that manufacturing companies need for their digital and intelligent development. At present, the influence mechanism behind impact of on companies’ performance still unclear lacks quantitative analysis. Based dynamic view, we propose a research model to investigate capability, innovation, value co-creation, company conduct empirical study based questionnaire data from 209 companies. The results show has significant positive performance. further innovation...
Hepatitis E virus (HEV), a non-enveloped, positive-stranded RNA virus, is transmitted in faecal-oral manner, and causes acute liver diseases humans. The HEV capsid made up of capsomeres consisting homodimers single structural protein forming the shell. These dimers are believed to protrude from viral surface interact with host cells initiate infection. To date, no information available for any proteins. Here, we report first time crystal structure domain E2s, protruding domain, together...
The current coronavirus disease 2019 (COVID-19) pandemic was the result of rapid transmission a highly pathogenic coronavirus, severe acute respiratory syndrome 2 (SARS-CoV-2), for which there is no efficacious vaccine or therapeutic. Toward development vaccine, here we expressed and evaluated as potential candidates four versions spike (S) protein using an insect cell expression system: receptor binding domain (RBD), S1 subunit, wild-type S ectodomain (S-WT), prefusion trimer-stabilized...
Tailed bacteriophages (order, Caudovirales) account for the majority of all phages. However, long flexible tail siphophages hinders comprehensive investigation mechanism viral gene delivery. Here, we report atomic capsid and in-situ structures machine marine siphophage, vB_DshS-R4C (R4C), which infects Roseobacter. The R4C virion, comprising 12 distinct structural protein components, has a unique five-fold vertex icosahedral that allows genome specific position interaction pattern tube...
Abstract The robust and stable expression of CD38 in T‐cell acute lymphoblastic leukemia (T‐ALL) blasts makes chimeric antigen receptor (CAR)‐T/natural killer (NK) a potential therapy for T‐ALL. However, normal T/NK cells causes fratricide CAR‐T/NK cells. Here “2‐in‐1” gene editing strategy is developed to generate fratricide‐resistant locus‐specific CD38‐specific CAR integrated into the disrupted locus by CRISPR/Cas9, placed under control either endogenous promoter ( KO/KI ) or exogenous...
Currently, SARS-CoV-2 has evolved into various variants, including the numerous highly mutated Omicron sub-lineages, significantly increasing immune evasion ability. The development raises concerns about possibly diminished effectiveness of available vaccines and antibody-based therapeutics. Here, we describe those representative categories broadly neutralizing antibodies (bnAbs) that retain prominent against emerging variants sub-lineages. molecular characteristics, epitope conservation,...
Matrix metalloproteinases (MMPs) are essential for the degradation and turnover of components extracellular matrix (ECM) and, when pathologically elevated, mediate connective tissue loss (including bone destruction) in various inflammatory other diseases. Tetracyclines (TCs) known inhibitors mammalian-derived MMPs, non-antibiotic formulations Doxycycline FDA-approved to treat periodontitis chronic skin disease, rosacea. Because C-11/ C-12 diketonic moiety tetracyclines is primarily...
Autophagy is a process for the turnover of intracellular organelles and molecules during stress responses. microRNAs (miRNAs) are small, non-coding endogenous RNAs that may regulate almost every cellular process. However, roles miRNAs in autophagy still poorly understood. In this study, we show miR-26a enhances both culture cells mouse liver. Hepatic overexpression mice alleviated ethanol-induced hepatic steatosis liver injury. Overexpression increased expression mediator Beclin-1, which...