A5101438468- CAR-T cell therapy research
- Acute Myeloid Leukemia Research
- Access Control and Trust
- Cloud Data Security Solutions
- Viral Infections and Immunology Research
- Parallel Computing and Optimization Techniques
- Hematopoietic Stem Cell Transplantation
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Distributed and Parallel Computing Systems
- Bacteriophages and microbial interactions
- Gastric Cancer Management and Outcomes
- Immune Cell Function and Interaction
- Hepatitis Viruses Studies and Epidemiology
- Acute Lymphoblastic Leukemia research
- Multiple and Secondary Primary Cancers
- Peer-to-Peer Network Technologies
- Herpesvirus Infections and Treatments
- Veterinary medicine and infectious diseases
- Intraperitoneal and Appendiceal Malignancies
- Metastasis and carcinoma case studies
- Glycosylation and Glycoproteins Research
- Liver Disease Diagnosis and Treatment
- Esophageal and GI Pathology
Zhejiang University
2003-2025
First Affiliated Hospital Zhejiang University
2021-2025
Xuzhou No.1 People's Hospital
2024
Xuzhou Medical College
2024
Beihang University
2023
Hengyang Normal University
2020
Guangxi Medical University
2020
Nantong University
2019
Xiamen University
2016-2019
King's College Hospital
2012-2019
ABSTRACT The limited infiltration and persistence of chimeric antigen receptor (CAR)-T cells is primarily responsible for their treatment deficits in solid tumors. Here, we present a three-dimensional scaffold, inspired by the physiological process T-cell proliferation lymph nodes. This scaffold gathers function loading, delivery, activation expansion CAR-T to enhance therapeutic effects on porous device made from poly(lactic-co-glycolic acid) microfluidic technique with modification...
Expansion of regulatory T cells occurs in high-risk myelodysplastic syndrome and correlates with a poor prognosis. DNA methyltransferase inhibitors, particularly 5-azacytidine, have been shown to increase the survival patients syndrome. It is not entirely clear whether this improvement patients’ related effects inhibitors on immune system and/or direct effect these drugs dysplastic clone. In study we investigated 5-azacytidine function proliferation capability T-helper cells. The number CD4+...
Chimeric antigen receptor T cell (CAR T) therapy was a milestone in the treatment of relapsed and refractory B malignancies. However, beneficial effects CAR cells have not been obtained solid tumors yet. Herein, we implement porous microneedle patch that accommodates allows situ penetration-mediated seeding when implanted tumor bed or post-surgical resection cavity. loaded pores tips were readily escorted to an evenly scattered manner without losing their activity. Such microneedle-mediated...
Abstract Coxsackievirus A6 (CVA6) has recently emerged as a major cause of hand, foot and mouth disease in children worldwide but no vaccine is available against CVA6 infections. Here, we demonstrate the isolation two forms stable particles-procapsid A-particle-with excellent biochemical stability natural antigenicity to serve candidates. Despite presence (in A-particle) or absence procapsid) capsid-RNA interactions, particles have essentially identical atomic capsid structures resembling...
Diagnostic criteria for hypoplastic myelodysplasic syndrome (h-MDS) have not been clearly established, making the differential diagnosis from other bone marrow failure syndromes (BMF) challenging. In this study, we aimed to delineate clinical, histopathological, and molecular features of h-MDS, based on a large well-annotated cohort patients with (BM) hypocellularity. The study included 534 consecutive adult hypocellular BM (278 h-MDS 136 aplastic anemia), 727 normo- or hypercellular MDS...
Abstract The robust and stable expression of CD38 in T‐cell acute lymphoblastic leukemia (T‐ALL) blasts makes chimeric antigen receptor (CAR)‐T/natural killer (NK) a potential therapy for T‐ALL. However, normal T/NK cells causes fratricide CAR‐T/NK cells. Here “2‐in‐1” gene editing strategy is developed to generate fratricide‐resistant locus‐specific CD38‐specific CAR integrated into the disrupted locus by CRISPR/Cas9, placed under control either endogenous promoter ( KO/KI ) or exogenous...
The exterior of bacteriophage T4 capsid is coated with two outer proteins, Hoc (highly antigenic protein; molecular mass, 40 kDa) and Soc (small 9 kDa), at symmetrical positions on the icosahedron (160 copies 960 per particle). Both these proteins are nonessential for phage infectivity viability assemble onto surface after completion assembly. We developed a display system which allowed in-frame fusions foreign DNA unique cloning site in 5' end hoc or soc. A fragment corresponding to...
With the rapid increase in demand for data trustworthiness and security, distributed storage technology represented by blockchain has received unprecedented attention. These technologies have been suggested various uses because of their remarkable ability to offer decentralization, high autonomy, full process traceability, tamper resistance. Blockchain enables exchange information value an untrusted environment. There a significant attention confidentiality privacy preservation technology....
In adaptive immunity, organisms produce neutralizing antibodies (nAbs) to eliminate invading pathogens. Here, we explored whether viral neutralization could be attained through the physical disruption of a virus upon nAb binding. We report mechanism potent 8C11 against hepatitis E (HEV), nonenveloped positive-sense single-stranded RNA associated with abundant acute hepatitis. The binding flanks protrusion spike HEV viruslike particles (VLPs) and leads tremendous collision between antibody...
BACKGROUND Primary liver cancer is the sixth most common worldwide, with hepatocellular carcinoma (HCC) being prevalent form. Despite current availability of multiple immune or combination treatment options, prognosis still poor, so how to identify a more suitable population extremely important. AIM To evaluate clinical effectiveness combining lenvatinib camrelizumab for patients hepatitis B virus (HBV)-related HCC in Barcelona Clinic Liver Cancer (BCLC) stages B/C, considering various body...
An experiment was conducted to investigate the effects of glutamine on growth performance weanling piglets. Sixty piglets weaned at 21 days age were randomly assigned two groups (10 per pen, 3 pens group). The control group received a maize-soybean meal-based diet. treatment diet supplemented with 1.0% free l-glutamine. Piglets fed diets for 20 days. Results showed that had lower diarrhoea ratio and shorter duration than those during after weaning. During first ten weaning, pigs 12.05%...
The HIV-1 mature capsid (CA) assumes an amorphous, fullerene conical configuration due to its high flexibility. How native CA self-assembles is still unclear despite having well-defined structures of pentamer and hexamer building blocks. Here we explored the self-assembly engineered protein built through artificial disulfide bonding (CA N21C/A22C) determined structure one fraction globular particles. N21C/A22C was found self-assemble into particles in relatively ionic solutions. These...
Abstract: Chimeric antigen receptor (CAR)-modified T cell therapy is increasingly administered for hematological malignancies. Cytokine release syndrome (CRS) a common and severe complication of CAR-T therapy. In the present case, 62-year-old male patient was diagnosed with relapsed refractory multiple myeloma (RRMM). Treated CART-CD19/BCMA therapy, his symptoms remitted, during which occasional but CRS associated coagulation disorder still appeared, as evidenced by coexistence huge...
A mechanosensitive ion channel, Piezo1 induces non-selective cation flux in response to various mechanical stresses. However, the biological interpretation and underlying mechanisms of cells resulting from activation remain elusive. This study elucidates Piezo1-mediated Ca 2+ influx driven by channel cellular behavior using novel Förster Resonance Energy Transfer (FRET)-based biosensors single-cell imaging analysis. Results reveal that extracellular via requires intact caveolin, cholesterol,...
Objective To evaluate the efficacy of three-dimensional conformal radiation therapy (3DCRT) for esophageal carcinoma and identify prognostic factors in this patient group.Methods From May 2002 to Jun 2005,132 patients with unresectable or inoperable cancer were treated 3DCRT our hospital.Their chnical data analyzed retrospectively.ResultsThe 1-and 2-year local control rates was 65.4% 52.1% whole group,respectively.The overall survival rate 50.7% 32.2% ,respectively.The median time 13...
With the development of grid service, there are two key problems to be improved. One is access Web service with friend interface for user. Another security authentication resource share. Nowadays, a solution universal entrance has been given through framework named portal. PKI based infrastructure widely used in order guarantee security. However, although proxy certificate well applied GSI, still no integrated model portal on and mechanism. From view cooperation, proposed online repository....
In this paper, we propose a fully decentralized authentication and revocation scheme(f-DMA) based on multi-authority attribute-based encryption that can be used in data sharing systems with multiple distrusted authorizes. This scheme establishes secret key generation distribution systems, which eliminates the security risk central authority(CA) compromise is resilient to authority user collusion attacks. Our system also provides computation efficient achieve both specific attribute...
In e-service environments, the delegation relationship is rarely considered as one of important factors trust models. Most work on focused permission delegatee, depth, constrain and so on. They ignored potential hazards brought from blind delegation, easily to form collusion attack. This paper defines concept trustworthiness firstly, then proposes a method for calculating based relationship. The enables involved delegator delegatee be responsible their actual behavior in process. delegate...