A5100346670- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- Virus-based gene therapy research
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Graphene and Nanomaterials Applications
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Parasites and Host Interactions
- Advanced Drug Delivery Systems
- Dendrimers and Hyperbranched Polymers
- Cancer Cells and Metastasis
- Sexual Differentiation and Disorders
- HIV Research and Treatment
- Circular RNAs in diseases
- Viral gastroenteritis research and epidemiology
- Cancer-related molecular mechanisms research
- Toxin Mechanisms and Immunotoxins
Zhejiang University
2020-2025
Second Affiliated Hospital of Zhejiang University
2022-2025
West China Hospital of Sichuan University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2009-2025
Sichuan University
2024-2025
Peking Union Medical College Hospital
2021-2025
Jinhua Academy of Agricultural Sciences
2023-2024
Czech Academy of Sciences, Institute of Biophysics
2024
Chinese Academy of Sciences
2004-2024
Center for NanoScience
2024
A principal goal of cancer nanomedicine is to deliver therapeutics effectively cells within solid tumors. However, there are a series biological barriers that impede from reaching target cells. Here, we report stimuli-responsive clustered nanoparticle systematically overcome these multiple by sequentially responding the endogenous attributes tumor microenvironment. The smart polymeric (iCluster) has an initial size ∼100 nm, which favorable for long blood circulation and high propensity...
The currently low delivery efficiency and limited tumor penetration of nanoparticles remain two major challenges cancer nanomedicine. Here, we report a class pH-responsive nanoparticle superstructures with ultrasensitive size switching in the acidic microenvironment for improved effective vivo drug delivery. were constructed from amphiphilic polymer directed assembly platinum-prodrug conjugated polyamidoamine (PAMAM) dendrimers, which contains ionizable tertiary amine groups rapid...
Although surface PEGylation of siRNA vectors is effective for preventing protein adsorption and thereby helps these to evade the reticuloendothelial system (RES) in vivo, it also suppresses cellular uptake by target cells. This dilemma could be overcome employing stimuli-responsive shell-detachable nanovectors achieve enhanced internalization while maintaining prolonged blood circulation. Among possible stimuli, dysregulated pH tumor (pHe) most universal practical. However, design...
The CRISPR/Cas9 gene editing technology holds promise for the treatment of multiple diseases. However, inability to perform specific in targeted tissues and cells, which may cause off-target effects, is one critical bottlenecks therapeutic application CRISPR/Cas9. Herein, macrophage-specific promoter-driven Cas9 expression plasmids (pM458 pM330) were constructed encapsulated cationic lipid-assisted PEG-b-PLGA nanoparticles (CLAN). obtained encapsulating able specifically express macrophages...
Chemoimmunotherapy, which combines chemotherapeutics with immune-modulating agents, represents an appealing approach for improving cancer therapy. To optimize its therapeutic efficacy, differentially delivering multiple drugs to target cells is desirable. Here we developed immunostimulatory nanocarrier (denoted as BLZ-945SCNs/Pt) that could spatially tumor-associated macrophages (TAMs) and tumor chemoimmunotherapy. BLZ-945SCNs/Pt undergo supersensitive structure collapse in the prevascular...
Liquid nitrogen–treated tumor cells serve as a drug carrier and cancer vaccine to facilitate chemo-immunotherapy.
Lipid nanoparticles (LNPs), a nonviral nucleic acid delivery system, have shown vast potential for vaccine development and disease treatment. LNPs assist mRNA to cross physiological barriers such as cell membranes endosomes/lysosomes, promoting the intracellular presentation of mRNA. However, endosome escape efficiency biosafety currently commercialized are still unsatisfactory, resulting in underutilization Herein, we report that fluorinated modification...
ABSTRACT The limited infiltration and persistence of chimeric antigen receptor (CAR)-T cells is primarily responsible for their treatment deficits in solid tumors. Here, we present a three-dimensional scaffold, inspired by the physiological process T-cell proliferation lymph nodes. This scaffold gathers function loading, delivery, activation expansion CAR-T to enhance therapeutic effects on porous device made from poly(lactic-co-glycolic acid) microfluidic technique with modification...
Although CRISPR-mediated genome editing holds promise for cancer therapy, inadequate tumor targeting and potential off-target side effects hamper its outcomes. In this study, we present a strategy using cryo-shocked lung cells as CRISPR-Cas9 delivery system cyclin-dependent kinase 4 ( CDK4 ) gene editing, which initiates synthetic lethal in KRAS-mutant non–small cell (NSCLC). By rapidly liquid nitrogen shocking, effectively eliminate the pathogenicity of while preserving their structure...
Positively charged nanoparticles showed a favorable distribution in the small intestine, and significantly improved oral bioavailability.
ConspectusImmune checkpoint blockade (ICB) therapy elicits antitumor response by inhibiting immune suppressor components, including programmed cell death protein 1 and its ligand (PD-1/PD-L1) cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4). Despite improved therapeutic efficacy, the clinical rate is still unsatisfactory as revealed fact that only a minority of patients experience durable benefits. Additionally, "off-target" effects after systemic administration remain challenging for...
Chimeric antigen receptor T cell (CAR T) therapy was a milestone in the treatment of relapsed and refractory B malignancies. However, beneficial effects CAR cells have not been obtained solid tumors yet. Herein, we implement porous microneedle patch that accommodates allows situ penetration-mediated seeding when implanted tumor bed or post-surgical resection cavity. loaded pores tips were readily escorted to an evenly scattered manner without losing their activity. Such microneedle-mediated...