A5011596319- CAR-T cell therapy research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Nanowire Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- T-cell and B-cell Immunology
- Protein Degradation and Inhibitors
- RNA Interference and Gene Delivery
- Biomedical and Engineering Education
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
Zhejiang University
2022-2024
Jinhua Academy of Agricultural Sciences
2024
ABSTRACT The limited infiltration and persistence of chimeric antigen receptor (CAR)-T cells is primarily responsible for their treatment deficits in solid tumors. Here, we present a three-dimensional scaffold, inspired by the physiological process T-cell proliferation lymph nodes. This scaffold gathers function loading, delivery, activation expansion CAR-T to enhance therapeutic effects on porous device made from poly(lactic-co-glycolic acid) microfluidic technique with modification...
Although CRISPR-mediated genome editing holds promise for cancer therapy, inadequate tumor targeting and potential off-target side effects hamper its outcomes. In this study, we present a strategy using cryo-shocked lung cells as CRISPR-Cas9 delivery system cyclin-dependent kinase 4 ( CDK4 ) gene editing, which initiates synthetic lethal in KRAS-mutant non–small cell (NSCLC). By rapidly liquid nitrogen shocking, effectively eliminate the pathogenicity of while preserving their structure...
Regulatory T (Treg) cells underlie multiple autoimmune disorders and potentialize an anti-inflammation treatment with adoptive cell therapy. However, systemic delivery of cellular therapeutics often lacks tissue targeting accumulation for localized diseases. Besides, the instability plasticity Treg also induce phenotype transition functional loss, impeding clinical translation. Here, we developed a perforated microneedle (PMN) favorable mechanical performance spacious encapsulation cavity to...
Abstract Hematopoietic progenitor pinase1 (HPK1) knockout has been identified as an efficient route to enhance anti‐tumor immune response. Here, this work develops oral proteolysis targeting chimera (PROTAC) HPK1 efficiently and selectively degrade augment immunotherapeutic outcomes. In a postoperative tumor model of human cervical cancer in NSG mice, the orally‐administrated PROTAC can reach tumors, down‐regulate levels locally‐administrated CAR‐T cells, promote their efficiency inhibiting...