A5091964183- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Advancements in Transdermal Drug Delivery
- Virus-based gene therapy research
- Advanced Drug Delivery Systems
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Lipid Membrane Structure and Behavior
- Immune Cell Function and Interaction
Zhejiang University
2023-2024
Although CRISPR-mediated genome editing holds promise for cancer therapy, inadequate tumor targeting and potential off-target side effects hamper its outcomes. In this study, we present a strategy using cryo-shocked lung cells as CRISPR-Cas9 delivery system cyclin-dependent kinase 4 ( CDK4 ) gene editing, which initiates synthetic lethal in KRAS-mutant non–small cell (NSCLC). By rapidly liquid nitrogen shocking, effectively eliminate the pathogenicity of while preserving their structure...
Regulatory T (Treg) cells underlie multiple autoimmune disorders and potentialize an anti-inflammation treatment with adoptive cell therapy. However, systemic delivery of cellular therapeutics often lacks tissue targeting accumulation for localized diseases. Besides, the instability plasticity Treg also induce phenotype transition functional loss, impeding clinical translation. Here, we developed a perforated microneedle (PMN) favorable mechanical performance spacious encapsulation cavity to...
The maintenance of stable plasma drug concentrations within a therapeutic window can be critical for efficacy. Here, we developed wearable osmotic microneedle (OMN) patch to support sustained dosing at least 24 hours without the use electronic components. OMN uses an pressure driving force deliver solution into skin through three hollow microneedles with diameters less than 200 micrometers. rate release was related composition and concentration osmogen physical properties semipermeable...