A5061408801- CAR-T cell therapy research
- RNA and protein synthesis mechanisms
- Cytokine Signaling Pathways and Interactions
- Phagocytosis and Immune Regulation
- interferon and immune responses
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Bacterial Genetics and Biotechnology
- Geotechnical Engineering and Underground Structures
- Nanowire Synthesis and Applications
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- Thyroid Cancer Diagnosis and Treatment
- Influenza Virus Research Studies
- Non-Destructive Testing Techniques
- Cancer Mechanisms and Therapy
- Chemokine receptors and signaling
- Seismic Performance and Analysis
- Nerve injury and regeneration
- S100 Proteins and Annexins
- Advanced Sensor Technologies Research
- Advancements in Semiconductor Devices and Circuit Design
- Neurogenesis and neuroplasticity mechanisms
- Smart Grid and Power Systems
- Optical Coherence Tomography Applications
Zhejiang University
2014-2025
Hangzhou Medical College
2024-2025
Zhejiang Provincial People's Hospital
2024-2025
Jiangnan University
2017-2022
First Affiliated Hospital Zhejiang University
2021-2022
Zhejiang Lab
2021
Bengbu Medical College
2018
Fuzhou General Hospital of Nanjing Military Command
2018
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2015
Liaoning University
2012-2015
Abstract Anaplastic thyroid carcinoma (ATC) has a 100% disease-specific mortality rate. The JAK1/2-STAT3 pathway presents promising target for treating hematologic and solid tumors. However, it is unknown whether the activated in ATC, anti-cancer effects mechanism of action its inhibitor, ruxolitinib (Ruxo, clinical JAK1/2 inhibitor), remain elusive. Our data indicated that signaling significantly upregulated ATC tumor tissues than normal papillary cancer tissues. Apoptosis GSDME-pyroptosis...
Abstract The robust and stable expression of CD38 in T‐cell acute lymphoblastic leukemia (T‐ALL) blasts makes chimeric antigen receptor (CAR)‐T/natural killer (NK) a potential therapy for T‐ALL. However, normal T/NK cells causes fratricide CAR‐T/NK cells. Here “2‐in‐1” gene editing strategy is developed to generate fratricide‐resistant locus‐specific CD38‐specific CAR integrated into the disrupted locus by CRISPR/Cas9, placed under control either endogenous promoter ( KO/KI ) or exogenous...
Abstract Objective This study assessed CLDN16 as a potential replacement or improvement biomarker for papillary thyroid cancer (PTC), addressing the limitations associated with prevalently used BRAF-V600E mutation. Design Database analyses, tissue validation, RNA sequencing, and functional assays were conducted to evaluate PTC its clinical application. Methods expression was examined in normal thyroid/para-tumor tissues compared across various types. We evaluated diagnostic accuracy,...
Abstract T cell genome editing holds great promise to advance a range of immunotherapies but is encumbered by the dependence on difficult‐to‐produce and expensive viral vectors. Here, small double‐stranded plasmid DNA modified mediate high‐efficiency homologous recombination designed. The resulting chimeric antigen receptor (CAR)‐T cells display similar phenotype, transcriptional profile, in vivo potency CAR‐T generated using adeno‐associated vector. This method should simplify accelerate...
The ribosome binding site (RBS) is a crucial element regulating translation. However, the activity of RBS poorly predictable, because it strongly affected by local possible secondary structure, that is, context dependence. By Flowseq technique, over 20 000 variants were sorted and sequenced, translation multiple genes under same was quantitatively characterized to evaluate dependence each variant in E. coli. Two regions, (−7 −2) (−17 −12), predicted with higher possibility pair other slow...
It is important to simulate the entire-process collapse of earthquake excited cable-stayed bridge in order assure damage extent under strong earthquakes and optimize anti-collapse seismic measures. The newly developed vector form intrinsic finite element (VFIFE) method capable computing large deformation, displacement, collision, fractures that would happen structural collapse. Therefore, it has potential structures. For bridges, axial forces change significantly during excitation....
TAM family members (TYRO3, AXL and MERTK) play essential roles in the resolution of inflammation infectious diseases cancer. AXL, a tyrosine kinase receptor, is commonly overexpressed several solid tumours numerous hematopoietic malignancies including acute myeloid leukaemia, lymphocytic chronic leukaemia multiple myeloma. significantly promotes tumour cell migration, invasion metastasis, as well angiogenesis. also plays an important role macrophage ontogeny. Recent studies have revealed...
Promoters and ribosome binding sites (RBSs) are routinely applied in gene expression regulation, but their orthogonality combinatorial effects have not yet been systematically studied Corynebacterium glutamicum. Here, 17 core promoters 29 RBSs C. glutamicum were characterized, which exhibited 470-fold 430-fold transcriptional translational activity, respectively. By comparing the of two reporter genes regulated by multiple RBSs, RBS efficacy showed significant dependence on context, besides...
Transcription terminators play a role in terminating the progress of gene transcription, and are thus essential elements circuit. Terminators have two main functions: transcription improving stability transcripts during translation. We therefore considered detailed characteristics relation to their different roles translation, including shut-down degree (α) upstream mRNA protection capacity (β), apparent termination efficiency (η) reflecting overall regulatory effect terminator. Based on...
CD19-targeting chimeric antigen receptors (CARs) with CD28 and CD3ζ signaling domains have been approved by the US FDA for treating B cell malignancies. Mutation of immunoreceptor tyrosine-based activation motifs (ITAMs) in generated a single-ITAM containing 1XX CAR, which displayed superior antitumor activity leukemia mouse model. Here, we investigated whether design could enhance therapeutic potency against solid tumors. We constructed both CD19- AXL-specific CARs compared their vitro vivo...
Nonstructural protein 1 (NS1) is a non-structural of avian influenza virus. It can interact with variety proteins the host cells, enhancing expression viral and changing growth metabolism thereby virus' pathogenicity virulence. To investigate whether there are more that NS1 during infection, T7-phage display system was used to screen human lung cell cDNA library for could NS1. One positive specific clone obtained identified as nucleolar coiled-body phosphoprotein 1(NOLC1). The interaction...
Abstract This paper presents a new approach to simulate the propagation of elastic and cohesive cracks under mode-I loading based on vector form intrinsic finite element method. The proposed can handle crack without requiring global stiffness matrices extra weak elements. structure is simulated by mass particles whose motions are governed Newton's second law. Elastic VFIFE- J -integral VFIFE-FCM methods, respectively. method (VFIFE) methods calculate stress intensity factors at tips,...
Olfactory ensheathing glia (OEG) play an important role in regulating the regeneration of injured nervous system. However, chronic inflammation damage reduces viability OEG via poorly understood mechanisms. We aimed to investigate pathological responses response LPS-mediated stress vitro. The results indicated that lipopolysaccharide (LPS) treatment significantly reduced a dose-dependent fashion. Mechanistically, LPS stimuli induced mitochondrial oxidative damage, fragmentation, metabolism...
The intrinsic terminator in prokaryotic forms secondary RNA structure and terminates the transcription. However, leaking transcription is common due to varied strength. Besides of representative hairpin U-tract structure, detailed sequence thermodynamic features terminators were not completely clear, effect on upstream gene expression was unclearly. Thus, it still challenging use control with higher precision. Here, E. Coli, we firstly determined 3'-end sequences including spacer downstream...
AXL receptor tyrosine kinase ligand (AXL), a that is commonly overexpressed in numerous types of cancer, significantly promotes drug resistance and metastasis tumor cells. Inhibition the AXL/growth arrest‑specific 6 (Gas6) signaling pathway emerging as potential anticancer therapeutic strategy. In present study, on basis three‑dimensional complex structure AXL/Gas6, critical residues (E56, E59 T77) binding to Gas6 were determined using computer graphics analysis distance geometry method....
Antibody resistance, both de novo and acquired, is usually related to high risk of recurrence lower survival rate in gynecologic cancers. Prevention or reversal the resistance often yields beneficial clinical results. It was reported that anti-human epidermal growth factor receptor 3 monoclonal antibody effective against trastuzumab-resistant breast cancer cells. Here our laboratory, an acquired ovarian cell line, SKOV3-T, established previously. Further, human observed be upregulated this...