A5017934141- Microbial Metabolic Engineering and Bioproduction
- Amino Acid Enzymes and Metabolism
- Biofuel production and bioconversion
- Enzyme Structure and Function
- Fungal and yeast genetics research
- Enzyme Catalysis and Immobilization
- Polyamine Metabolism and Applications
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Metabolism and Genetic Disorders
- Cardiac Valve Diseases and Treatments
- Neuroscience and Neuropharmacology Research
- Caveolin-1 and cellular processes
- Cancer Research and Treatments
- Biopolymer Synthesis and Applications
- Acupuncture Treatment Research Studies
- Sulfur Compounds in Biology
- Electrolyte and hormonal disorders
- Genetic Mapping and Diversity in Plants and Animals
- Microbial metabolism and enzyme function
- Biotin and Related Studies
- Autophagy in Disease and Therapy
- Genetics and Plant Breeding
First Affiliated Hospital Zhejiang University
2025
Henan Agricultural University
2025
Jiangnan University
2015-2024
Jiangsu Industry Technology Research Institute
2024
Yixing People's Hospital
2023
Jiangsu University
2023
Zhejiang University
2022
Hangzhou Medical College
2022
Zhejiang Provincial People's Hospital
2022
Chinese Academy of Sciences
2022
Abstract Background Fumaric acid is a commercially important component of foodstuffs, pharmaceuticals and industrial materials, yet the current methods production are unsustainable ecologically destructive. Results In this study, fumarate biosynthetic pathway involving reductive reactions tricarboxylic cycle was exogenously introduced in S. cerevisiae by series simple genetic modifications. First, Rhizopus oryzae genes for malate dehydrogenase ( RoMDH ) fumarase RoFUM1 were heterologously...
Abstract S‐Adenosyl‐L‐methionine (SAM) is a substrate for many enzyme‐catalyzed reactions and provides methyl groups in numerous biological methylations, thus has vast applications the agriculture medical field. Saccharomyces cerevisiae been engineered as platform with significant potential producing SAM, but current production room improvement. Thus, method that consists of series metabolic engineering strategies was established this study. These included enhancing SAM synthesis, increasing...
Fumaric acid (FA) is a promising biomass-derived building-block chemical. Bio-based FA production from renewable feedstock and sustainable alternative to petroleum-based chemical synthesis. Here we report on by direct fermentation using metabolically engineered Saccharomyces cerevisiae with the aid of in silico analysis genome-scale metabolic model. First, FUM1 was selected as target gene basis extensive literature mining. Flux balance (FBA) revealed that deletion can lead slightly lower...
Abstract Differences in the distribution of hydrophilic and hydrophobic bile acids (BA) are observed mouse models non‐alcoholic fatty liver disease (NAFLD) induced by a high‐fat‐cholesterol “Western‐style” diet (WD), cholesterol gallstone (CGD) lithogenic diet. Despite sharing common pathological processes, these exhibit distinct characteristics their BA pools. The study investigates impact ( Hpho BA) Hphil on CGD development using cytochrome‐P450‐2c70 knockout (C70‐KO) mice (mice C70‐KO ),...
SUMMARY The optimal plant architecture, characterized by short stature, helps mitigate lodging, enables high‐density planting, and facilitates mechanized harvesting. Internode length (IL), a crucial component of height in maize, plays significant role these processes. However, the genetic mechanisms underlying internode elongation remain poorly understood. In this study, we conducted genome‐wide association study to dissect architecture IL maize. lengths five internodes above below ear...
Abstract Background l -Serine has wide and increasing applications in industries with fast-growing market demand. Although strategies for achieving improving -serine production Corynebacterium glutamicum ( C. ) have focused on inhibiting its degradation enhancing biosynthetic pathway, yield remained relatively low. Exporters play an essential role the fermentative of amino acids. To achieve higher yield, export from cell should be improved. In , ThrE, which can -threonine -serine, is only...
The ribosome binding site (RBS) is a crucial element regulating translation. However, the activity of RBS poorly predictable, because it strongly affected by local possible secondary structure, that is, context dependence. By Flowseq technique, over 20 000 variants were sorted and sequenced, translation multiple genes under same was quantitatively characterized to evaluate dependence each variant in E. coli. Two regions, (−7 −2) (−17 −12), predicted with higher possibility pair other slow...
l ‐Serine is widely used in the pharmaceutical, food, and cosmetic industries. The direct fermentative production of ‐serine from glucose Corynebacterium glutamicum ( C. ) has been achieved but yield generally low. In this study, central metabolic pathway rewired to higher accumulation glycerate‐3‐phosphate (3‐PG), precursor for biosynthesis, thereby freeing up more carbon enhance yield. To end, native phosphotransferase system (PTS) inactivated by deleting gene ptsH. Meanwhile,...
Metabolic engineering of Saccharomyces cerevisiae for high-yield production carboxylic acid requires a cytosolic pyruvate pool as precursor. In this study, novel strategy to improve and reduce metabolic by-products via regulating thiamine synthesis was explored. Two the biosynthesis regulatory genes, THI2 THI3, were disrupted in S. parent strain FMME-002. The mutants FMME-002ΔTHI2 FMME-002ΔTHI3 both exhibited an enhanced yield. Moreover, achieved relatively higher production, highest...
Abstract The issue of bacterial resistance has become a matter significant concern globally, prompting extensive research into the development and clinical application aminoglycoside antibiotics (AGAs) as crucial antibacterial therapy. One primary mechanisms contributing to antibiotic is action modifying enzymes (AMEs), which modify consequently confer drug resistance. Chemical modification AME site can eliminate functional groups vulnerable AMEs, thus improving This study involves...
The intrinsic terminator in prokaryotic forms secondary RNA structure and terminates the transcription. However, leaking transcription is common due to varied strength. Besides of representative hairpin U-tract structure, detailed sequence thermodynamic features terminators were not completely clear, effect on upstream gene expression was unclearly. Thus, it still challenging use control with higher precision. Here, E. Coli, we firstly determined 3'-end sequences including spacer downstream...