A5045803827- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Plant biochemistry and biosynthesis
- Biofuel production and bioconversion
- Microbial Natural Products and Biosynthesis
- Gene Regulatory Network Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Metabolomics and Mass Spectrometry Studies
- Algal biology and biofuel production
- Bacterial Genetics and Biotechnology
- Microbial metabolism and enzyme function
- Biochemical Acid Research Studies
- Photosynthetic Processes and Mechanisms
- Evolution and Genetic Dynamics
- Physiological and biochemical adaptations
- Marine Sponges and Natural Products
- Plant Gene Expression Analysis
- Steroid Chemistry and Biochemistry
- Essential Oils and Antimicrobial Activity
- Fungal and yeast genetics research
- Neurobiology and Insect Physiology Research
- Bioinformatics and Genomic Networks
- Amino Acid Enzymes and Metabolism
- Analytical Chemistry and Chromatography
- Antioxidant Activity and Oxidative Stress
East China University of Science and Technology
2015-2024
Shanghai Innovative Research Center of Traditional Chinese Medicine
2015-2019
Shanghai Institute of Organic Chemistry
2013
Chinese Academy of Sciences
2013
Fujian Medical University
2007-2010
Wuhan University
2007
University of California, San Diego
2006-2007
La Jolla Bioengineering Institute
2007
Keio University
2001-2004
Institute of Microbiology
2001
Limonene, a monocyclic monoterpene, is known for its using as an important precursor of many flavoring, pharmaceutical, and biodiesel products. Currently, d-limonene has been produced via fractionation from essential oils or byproduct orange juice production, however, considering the increasing need limonene certain amount pesticides may exist in obtained citrus industry, some other methods should be explored to produce limonene.To construct synthetic pathway Yarrowia lipolytica, two genes...
Squalene is a high-value antioxidant with many commercial applications. The use of microbial cell factories to produce squalene as an alternative plant and animal extracts could meet increasing market demand. Yarrowia lipolytica excellent host for production due its high levels acetyl-CoA hydrophobic environment. However, the need precise complicated gene editing has hindered industrialization this strain. Herein, rapid construction strain was achieved by enhancing homologous recombination...
The responses of Escherichia coli central carbon metabolism to knockout mutations in phosphoglucose isomerase and glucose-6-phosphate (G6P) dehydrogenase genes were investigated by using glucose- ammonia-limited chemostats. metabolic network structures intracellular fluxes the wild type mutants characterized complementary methods flux ratio analysis based on [U-(13)C]glucose labeling two-dimensional nuclear magnetic resonance (NMR) spectroscopy cellular amino acids, glycerol, glucose....
ABSTRACT Studies of steady-state metabolic fluxes in Escherichia coli grown nutrient-limited chemostat cultures suggest remarkable flux alterations response to changes growth-limiting nutrient the medium (Hua et al., J. Bacteriol. 185: 7053-7067, 2003). To elucidate physiological adaptation cells condition through change and understand molecular mechanisms underlying flux, information on gene expression is great importance. DNA microarray analysis was performed investigate global...
With the emergence of energy scarcity, use renewable sources such as biodiesel is becoming increasingly necessary. Recently, many researchers have focused their minds on Yarrowia lipolytica, a model oleaginous yeast, which can be employed to accumulate large amounts lipids that could further converted biodiesel. In order understand metabolic characteristics Y. lipolytica at systems level and examine potential for enhanced lipid production, genome-scale compartmentalized network was...
Squalene, a valuable acyclic triterpene, can be used as chemical commodity for pharmacology, flavor, and biofuel industries. Microbial production of squalene has been great interest due to its limited availability, increasing prices extracted from animal plant tissues. Here we report genetic perturbations that synergistically improve in Saccharomyces cerevisiae. As reported previously, overexpression truncated HMG-CoA reductase 1 (tHMG1) led the accumulation 20-fold higher than parental...
Recent advances in the production of biofuels by microbes have attracted attention due to increasingly limited fossil fuels. Biodiesels, especially fatty acid ethyl esters (FAEEs), are considered a potentially fully sustainable fuel near future similarities with petrodiesels and compatibility existing infrastructure. However, biosynthesis FAEEs is supply precursor lipids acetyl-CoA. In present study, we explored potential an engineered biosynthetic pathway coupled addition ethanol oleaginous...
Cellular interactions are a major driver for the assembly and functioning of microbial communities. Their strengths shown to be highly variable in nature; however, it is unclear how such variations regulate community behaviors. Here we construct synthetic Lactococcus lactis consortia mathematical models elucidate role interaction variability ecosystem succession further determine if casting into modeling empowers bottom-up predictions. For consortium bacteriocin-mediated cooperation...
α-Pinene, an important biologically active natural monoterpene, has been widely used in fragrances, medicines, and fine chemicals, especially, high-density renewable fuels such as jet fuel. The development of α-pinene production platform a highly modifiable microbe from substitute feedstocks could lead to green, economical avenue, sustainable biotechnological process for the biosynthesis α-pinene. Here, we report engineering orthogonal biosynthetic pathway efficient oleaginous yeast Yarrowia...
Excessive use of traditional food preservatives has raised concerns about their adverse effects. α-Copaene is a natural terpene compound with excellent biological activity; however, the antibacterial activity and mechanism α-copaene are unclear. Therefore, this study aimed to explore action against four common foodborne pathogens: Staphylococcus aureus, Escherichia coli, Bacillus cereus, Shigella bogdii. inhibited bacterial growth minimum inhibitory concentration 0.5–1 μL/mL bactericidal 2–4...
Abstract The gluconeogenic phosphoenolpyruvate (PEP) carboxykinase is active in Escherichia coli during its growth on glucose. present study investigated the influence of rates and PEP knockout anaplerotic fluxes E. . intracellular were determined using complementary methods flux ratio analysis metabolic based [U‐ 13 C 6 ]glucose labeling experiments 2D nuclear magnetic resonance (NMR) spectroscopy cellular amino acids glycerol. Significant activity was identified wild‐type , ATP dissipation...
Limonene, a valuable cyclic monoterpene, has been broadly studied in recent decades due to its wide application the food, cosmetics and pharmaceutical industries. Engineering of yeast Yarrowia lipolytica for fermentation renewable biomass lignocellulosic hydrolysate may reduce cost improve economics bioconversion production limonene. The aim this study was engineer Y. produce limonene from xylose low-cost feedstock. heterologous genes XR XDH native gene XK encoding assimilation enzymes,...