A5042699380- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Plant biochemistry and biosynthesis
- Microbial metabolism and enzyme function
- Biochemical Acid Research Studies
- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Algal biology and biofuel production
- Fungal and yeast genetics research
- Protist diversity and phylogeny
- Photosynthetic Processes and Mechanisms
- Porphyrin Metabolism and Disorders
- Gene Regulatory Network Analysis
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Analytical Chemistry and Chromatography
- Antioxidant Activity and Oxidative Stress
- Fungal Biology and Applications
- Marine Sponges and Natural Products
- Lipid metabolism and biosynthesis
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Microbial bioremediation and biosurfactants
East China University of Science and Technology
2015-2024
University of Illinois Urbana-Champaign
2018
Limonene, a monocyclic monoterpene, is known for its using as an important precursor of many flavoring, pharmaceutical, and biodiesel products. Currently, d-limonene has been produced via fractionation from essential oils or byproduct orange juice production, however, considering the increasing need limonene certain amount pesticides may exist in obtained citrus industry, some other methods should be explored to produce limonene.To construct synthetic pathway Yarrowia lipolytica, two genes...
Squalene, a valuable acyclic triterpene, can be used as chemical commodity for pharmacology, flavor, and biofuel industries. Microbial production of squalene has been great interest due to its limited availability, increasing prices extracted from animal plant tissues. Here we report genetic perturbations that synergistically improve in Saccharomyces cerevisiae. As reported previously, overexpression truncated HMG-CoA reductase 1 (tHMG1) led the accumulation 20-fold higher than parental...
Recent advances in the production of biofuels by microbes have attracted attention due to increasingly limited fossil fuels. Biodiesels, especially fatty acid ethyl esters (FAEEs), are considered a potentially fully sustainable fuel near future similarities with petrodiesels and compatibility existing infrastructure. However, biosynthesis FAEEs is supply precursor lipids acetyl-CoA. In present study, we explored potential an engineered biosynthetic pathway coupled addition ethanol oleaginous...
α-Pinene, an important biologically active natural monoterpene, has been widely used in fragrances, medicines, and fine chemicals, especially, high-density renewable fuels such as jet fuel. The development of α-pinene production platform a highly modifiable microbe from substitute feedstocks could lead to green, economical avenue, sustainable biotechnological process for the biosynthesis α-pinene. Here, we report engineering orthogonal biosynthetic pathway efficient oleaginous yeast Yarrowia...
Limonene, a valuable cyclic monoterpene, has been broadly studied in recent decades due to its wide application the food, cosmetics and pharmaceutical industries. Engineering of yeast Yarrowia lipolytica for fermentation renewable biomass lignocellulosic hydrolysate may reduce cost improve economics bioconversion production limonene. The aim this study was engineer Y. produce limonene from xylose low-cost feedstock. heterologous genes XR XDH native gene XK encoding assimilation enzymes,...
β-Carotene is an indispensable additive in beverage, cosmetic, feed, and pharmaceutical production. The fermentation industry annually generates abundant waste mycelia from Trichoderma reesei (T. reesei), a pivotal industrial strain for cellulase heterologous protein In this study, we constructed T. cell factory β-carotene production the first time. Four key enzymes, CarRP, CarB, GGS1/CrtE, HMG1, were overexpressed reesei. concentrations of medium components, including tryptone glucose,...
Foam cell formation has traditionally been attributed to macrophages; however, emerging evidence highlights vascular smooth muscle cells (VSMCs) as another significant contributor. Here, we found TMEM41B is significantly upregulated in VSMCs of both human atherosclerotic (AS) lesions and murine models. specific silencing expression apolipoprotein E-deficient (ApoE-/-) mice markedly reduced plaque size macrophage infiltration. Overexpressing cultured alters intracellular lipid profiles...
Squalene is a triterpenoid serving as an ingredient of various products in the food, cosmetic, pharmaceutical industries. The oleaginous yeast Yarrowia lipolytica offers enormous potential microbial chassis for production terpenoids, such carotenoid, limonene, linalool, and farnesene, provides ample storage space hydrophobic products. Here, we present metabolic design that allows enhanced accumulation squalene Y. lipolytica. First, improved by overexpressing genes (
T. reesei is commonly utilized in industry to produce cellulases, enzymes that degrade lignocellulosic biomass for the production of bioethanol and bio-based products. capable rapidly initiating biosynthesis cellulases presence cellulose, which has made it useful as a model fungus studying gene expression eukaryotes. Cellulase controlled through multiple transcription factors at transcriptional level.
Abstract Engineering Yarrowia lipolytica to produce astaxanthin provides a promising route. Here, Y. M2 producing titer of 181 mg/L was isolated by iterative atmospheric and room‐temperature plasma mutagenesis diphenylamine‐mediated screening. Interestingly, negative correlation observed between cell biomass production. To reveal the underlying mechanism, RNA‐seq analysis transcriptional changes performed in high producer reference strain M1, total 1379 differentially expressed genes were...
Genome-scale metabolic models embody a significant advantage of systems biology since their applications as flux simulation enable predictions for the production industrially-interesting metabolites. The biotechnological lycopene from Yarrowia lipolytica is an emerging scope that has not been fully scrutinized, especially what concerns cultivation conditions newly generated engineered strains. In this study, by combining balance analysis (FBA) and Plackett-Burman design, we screened...
As a novel chemical production platform, controllable and inducible modules in Synechococcus elongatus plus the ability of working diurnal conditions are necessary. To endeavors, promoters, such as PTrc, have been refined from Escherichia coli, but inducer isopropyl-β-D-thiogalactoside may cause several side-effects. Meanwhile, to promote efficiency, photomixotrophic cultivation has applied S. with additional organic carbon sources. In this study, we developed L-arabinose based consisted...
Abstract Background The filamentous fungus Trichoderma reesei is one of the best producers cellulase and has been widely studied for production cellulosic ethanol bio-based products. We previously reported that Mn 2+ N , -dimethylformamide (DMF) can stimulate overexpression via Ca bursts calcium signalling in T. under cellulase-inducing conditions. To further understand regulatory networks involved we characterised /DMF-induced pathway stimulation overexpression. Results found /DMF...
Ansamitocin P-3 (AP-3), produced by Actinosynnema pretiosum, has extraordinary antitumor activity and been used as toxic "warhead" in antibody-conjugated drugs. However, AP-3 production is limited its low yield thus the high cost of fermentation. In this study, we aimed to reduce fermentation costs utilizing low-cost substrates improve production, simultaneously. for ansamitocins were selected via single-factor experiments then concentrations these suitable carbon nitrogen sources optimized...