A5103108830- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Fungal and yeast genetics research
- Enzyme Structure and Function
- Polyamine Metabolism and Applications
- Antifungal resistance and susceptibility
- Bacterial Genetics and Biotechnology
- Probiotics and Fermented Foods
- Polysaccharides and Plant Cell Walls
- Microbial Natural Products and Biosynthesis
- Enzyme Production and Characterization
- Microbial metabolism and enzyme function
- Plant biochemistry and biosynthesis
- Peptidase Inhibition and Analysis
- Microbial Metabolism and Applications
- Biochemical Acid Research Studies
- CO2 Reduction Techniques and Catalysts
- Microbial Metabolites in Food Biotechnology
- Plant tissue culture and regeneration
- Alkaline Phosphatase Research Studies
- Wastewater Treatment and Nitrogen Removal
- Vitamin C and Antioxidants Research
- Fungal Infections and Studies
- Endoplasmic Reticulum Stress and Disease
Jiangnan University
2015-2024
State Key Laboratory of Food Science and Technology
2014-2023
Synthetic Biologics (United States)
2020
Abstract The application of rational design in reallocating metabolic flux to overproduce desired chemicals is always restricted by the native regulatory network. Here, we demonstrated that vitro modular pathway optimization combined with vivo multiplexed combinatorial engineering enables effective characterization bottleneck a complex biosynthetic cascade and improves output engineered pathway. As proof concept, systematically identified rate‐limiting step five‐gene malate combinatorially...
A four-carbon dicarboxylic acid L-malate has recently attracted attention due to its potential applications in the fields of medicine and agriculture. In this study, Escherichia coli W3110 was engineered optimized for production via one-step synthesis pathway. First, deletion genes encoding lactate dehydrogenase (ldhA), pyruvate oxidase (poxB), formate lyase (pflB), phosphotransacetylase (pta), acetate kinase (ackA) pta-ackA pathway led accumulate 20.9 g/L pyruvate. Then, overexpression...
Fumaric acid (FA) is a promising biomass-derived building-block chemical. Bio-based FA production from renewable feedstock and sustainable alternative to petroleum-based chemical synthesis. Here we report on by direct fermentation using metabolically engineered Saccharomyces cerevisiae with the aid of in silico analysis genome-scale metabolic model. First, FUM1 was selected as target gene basis extensive literature mining. Flux balance (FBA) revealed that deletion can lead slightly lower...
ABSTRACT A multi‐vitamin auxotrophic Torulopsis glabrata strain, a pyruvate producer, was further engineered to produce fumaric acid. Using the genome‐scale metabolic model i NX804 of T. , four acid biosynthetic pathways, involving cytosolic enzymes, argininosuccinate lyase (ASL), adenylosuccinate (ADSL), fumarylacetoacetase (FAA), and fumarase (FUM1), were found. Athough single overexpression each enzymes in cytosol improved production, highest titer (5.62 g L −1 ) obtained with strain...
Candida glabrata is a promising producer of organic acids. To elucidate the physiological function Mediator tail subunit Med15B in response to low-pH stress, we constructed deletion strain, C. glabratamed15BΔ, and an overexpression HTUΔ/CgMED15B Deletion MED15B caused biomass production, glucose consumption rate, cell viability decrease by 28.3%, 31.7%, 26.5%, respectively, compared with those parent (HTUΔ) strain at pH 2.0. Expression lipid metabolism-related genes was significantly...
Tryptophan, an essential aromatic amino acid, is widely used in animal feed, food additives, and pharmaceuticals. Although sustainable environmentally friendly, microbial tryptophan production from renewable feedstocks limited by low biosynthesis transport rates. Here, Escherichia coli strain capable of efficient was generated improving balancing the supply precursors engineering membrane transporters. Tryptophan increased eliminating negative regulatory factors, blocking competing pathways,...
The compartmentalization of enzymes into organelles is a promising strategy for limiting metabolic crosstalk and improving pathway efficiency; however, prokaryotes are unicellular organisms that lack membrane-bound organelles. To mimic this natural compartmentalization, we present here the targeting reductive tricarboxylic acid (rTCA) to periplasm enhance production malate. A multigene combination knockout was used construct phosphoenolpyruvate (PEP) pool. Then, genes encoding carboxykinase...
The production of l-leucine was improved by the disruption ltbR encoding transcriptional regulator and overexpression key genes (leuAilvBNCE) biosynthesis pathway in Corynebacterium glutamicum XQ-9. In order to improve production, we rationally engineered C. enhance improving redox flux. On basis this, manipulated state cells mutating coenzyme-binding domains acetohydroxyacid isomeroreductase encoded ilvC, inserting NAD-specific leucine dehydrogenase, leuDH from Lysinibacillus sphaericus,...
Abstract Aspergillus oryzae is a competitive natural producer for organic acids, but its production capacity closely correlated with specific morphological type. Here, morphology engineering was used tailoring A. to enhance l ‐malate production. Specifically, correlation between and fermentation first conducted, the optimal range of total volume pellets in unit broth (V value) 120–130 mm 3 /ml. To achieve this range, improved by controlling variation operational parameters, such as agitation...
The asexual facultative aerobic haploid yeast Candida glabrata is widely used in the industrial production of various organic acids. To elucidate physiological function C. transcription factor Crz1p (CgCrz1p) and its role tolerance to acid stress, we deleted or overexpressed corresponding gene, CgCRZ1 Deletion resulted a 60% decrease dry weight cells (DCW) 50% drop cell viability compared with those wild type at pH 2.0. Expression lipid metabolism-associated genes was also significantly...
Candida glabrata is a promising microorganism for organic acid production. The present study aimed to investigate the role of C. Mediator complex subunit 3 (CgMed3p) in protecting under low-pH conditions. To this end, genes CgMED3A and CgMED3B were deleted, resulting double-deletion Cgmed3ABΔ strain. final biomass cell viability levels decreased by 64.5% 35.8%, respectively, compared wild-type strain results at pH 2.0. In addition, lack CgMed3ABp resulted selective repression subset lipid...
α-Ketoglutaric acid (α-KG) is a multifunctional dicarboxylic in the tricarboxylic (TCA) cycle, but microbial engineering for α-KG production not economically efficient, due to intrinsic inefficiency of its biosynthetic pathway. In this study, pathway was used improve efficiency Escherichia coli. First, TCA cycle rewired starting from pyruvate, and engineered strain E. coli W3110Δ4-PCAI produced 15.66 g/L α-KG. Then, optimized by designing various strengths pyruvate carboxylase isocitrate...
Candida glabrata is an important microorganism used in commercial fermentation to produce pyruvate, but very little known about its mechanisms for surviving acid stress culture. In this study, it was shown that transcription factors Asg1p and Hal9p play essential roles C. the tolerance of stress, as deletion CgASG1 or CgHAL9 resulted inability survive acidic environment. Cgasg1Δ Cghal9Δ mutant strains are unable maintain pH homeostasis, evidenced by a decrease intracellular increase reactive...