A5060332476- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Bacterial Genetics and Biotechnology
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Biofuel production and bioconversion
- Amino Acid Enzymes and Metabolism
- Photosynthetic Processes and Mechanisms
- Gene Regulatory Network Analysis
- Carbohydrate Chemistry and Synthesis
- Photoreceptor and optogenetics research
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Algal biology and biofuel production
- Plant biochemistry and biosynthesis
- Microbial Fuel Cells and Bioremediation
- Fungal and yeast genetics research
- Cancer Research and Treatments
- CO2 Reduction Techniques and Catalysts
- Protist diversity and phylogeny
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Polyamine Metabolism and Applications
- Click Chemistry and Applications
- Electrochemical sensors and biosensors
Jiangnan University
2017-2025
Affiliated Hospital of Qingdao University
2024
Qingdao University
2024
ETH Zurich
2024
State Key Laboratory of Food Science and Technology
2017-2022
Capital Normal University
2015-2021
University of Manitoba
1972
University of Alabama at Birmingham
1972
Microbial utilization of methanol for valorization is an effective way to advance green bio-manufacturing technology. Although synthetic methylotrophs have been developed, strategies enhance their cell growth rate and internal regulatory mechanism remain underexplored. In this study, we design a assimilation (SMA) pathway containing only six enzymes linked central carbon metabolism, which does not require energy emissions. Through rational laboratory evolution, E. coli harboring with the SMA...
Significance The genome analysis presented here represents a major step forward in the field of desiccation tolerance and much-anticipated resource that will have far-reaching effect many areas plant biology agriculture. We present ∼1.69-Gb draft Boea hygrometrica , an important model for understanding responses to dehydration. To our knowledge, this is first sequence desiccation-tolerant extremophile, offering insight into evolution trait look, organization tolerance. underpinning...
Synthetic biology aims to develop programmable tools perform complex functions such as redistributing metabolic flux in industrial microorganisms. However, development of protein-level circuits is limited by availability designable, orthogonal, and composable tools. Here, with the aid engineered viral proteases proteolytic signals, we build two sets controllable protein units, which can be rationally configured three Using a protease-based dynamic regulation circuit fine-tune flow, achieve...
Abstract Cell division can perturb the metabolic performance of industrial microbes. The C period cell starts from initiation to termination DNA replication, whereas D is bacterial process. Here, we first shorten and periods E. coli by controlling expression ribonucleotide reductase NrdAB proteins FtsZA through blue light near-infrared activation, respectively. It increases specific surface area 3.7 μm −1 acetoin titer 67.2 g·L . Next, prolong regulating NrdA protein inhibitor SulA...
Abstract The application of rational design in reallocating metabolic flux to overproduce desired chemicals is always restricted by the native regulatory network. Here, we demonstrated that vitro modular pathway optimization combined with vivo multiplexed combinatorial engineering enables effective characterization bottleneck a complex biosynthetic cascade and improves output engineered pathway. As proof concept, systematically identified rate‐limiting step five‐gene malate combinatorially...
Abstract Saccharomyces cerevisiae is widely employed as a cell factory for the production of biofuels. However, product toxicity has hindered improvements in biofuel production. Here, we engineer actin cytoskeleton S. to increase both growth and n-butanol medium-chain fatty acids. Actin cable tortuosity regulated using an responsive promoter-based autonomous bidirectional signal conditioner . The budding index increased by 14.0%, resulting highest titer 1674.3 mg L −1 Moreover, patch density...
P-coumaric acid (p-CA), a pant metabolite with antioxidant and anti-inflammatory activity, is extensively utilized in biomedicine, food, cosmetics industry. In this study, synthetic pathway (PAL) for p-CA was designed, integrating three enzymes (AtPAL2, AtC4H, AtATR2) into higher l-phenylalanine-producing strain Escherichia coli PHE05. However, the lower soluble expression activity of AtC4H PAL bottleneck increasing titers. To overcome limitation, enhanced through N-terminal modifications....
Tryptophan, an essential aromatic amino acid, is widely used in animal feed, food additives, and pharmaceuticals. Although sustainable environmentally friendly, microbial tryptophan production from renewable feedstocks limited by low biosynthesis transport rates. Here, Escherichia coli strain capable of efficient was generated improving balancing the supply precursors engineering membrane transporters. Tryptophan increased eliminating negative regulatory factors, blocking competing pathways,...
Biomass formation and product synthesis decoupling have been proven to be promising increase the titer of desired value add products. Optogenetics provides a potential strategy develop light-induced circuits that conditionally control metabolic flux redistribution for enhanced microbial production. However, limited number light-sensitive proteins available date hinders progress light-controlled tools.To address these issues, two optogenetic systems (TPRS TPAS) were constructed by...
Microbial cell factories using a single carbon source (e.g., sugars) have been used to produce wide variety of chemicals. However, this process is often accompanied by stoichiometric constraints on carbons and redox cofactors. Here, synthetic pathway was designed constructed in Escherichia coli synergistically use glucose formate as mixed sources. By optimizing via enzyme mining, protein engineering, bioprocess approaches, the yield pyruvate from enhanced 94% theoretical glycolysis yield,...
Cytochrome P450 enzymes (CYPs) catalyze a series of C-H and C=C oxygenation reactions, including hydroxylation, epoxidation, ketonization. They are attractive biocatalysts because their ability to selectively introduce oxygen into inert molecules under mild conditions. This review provides comprehensive overview the reactions catalyzed by CYPs various strategies for achieving higher selectivity enzymatic activity. Furthermore, we discuss application obtain desired chemicals or pharmaceutical...
l-Asparagine (l-Asn) is an important amino acid with broad applications in food, medicine, fine chemicals, and environmental protection. However, its industrial production limited by the high cost of raw materials low catalytic efficiency enzymes. In this study, a three-enzyme cascade pathway (FDN) was designed to produce l-Asn, using fumaric as material. Within pathway, EcAsnA identified rate-limiting enzyme subsequently engineered product rescue strategy reduce inhibition opening closed...
Indigo is widely used in dyes, medicines and semiconductors materials due to its excellent dyeing efficiency, antibacterial, antiviral, anticancer, anti‐corrosion, thermostability properties. Here, a biosynthetic pathway for indigo was designed, integrating two enzymes (EcTnaA, MaFMO) into higher L‐tryptophan‐producing the strain Escherichia coli TRP. However, lower catalytic activity of MaFMO bottleneck increasing titers. To overcome this limitation, enzyme enhanced through mechanism‐guided...
Epoxides, a class of ethers with three-membered ring structure, are widely used in the textile, pharmaceutical, and packaging industries. Chemoenzymatic epoxidation presents promising method for synthesizing epoxides. However, its efficiency is hindered by low chemoselective perhydrolysis, which caused hydrolysis side reaction aqueous phase. In this study, chemoenzymatic process phase was developed utilizing an acyltransferase from P. litoralis (PlAcT) perhydrolysis. Crystal structure...