A5031328110- Foot and Ankle Surgery
- Tendon Structure and Treatment
- Knee injuries and reconstruction techniques
- Sports injuries and prevention
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Total Knee Arthroplasty Outcomes
- Osteoarthritis Treatment and Mechanisms
- Lower Extremity Biomechanics and Pathologies
- Cell death mechanisms and regulation
- Orthopedic Surgery and Rehabilitation
- interferon and immune responses
- Cancer-related Molecular Pathways
- Liver Disease Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- Inflammasome and immune disorders
- Viral Infections and Vectors
- Periodontal Regeneration and Treatments
- Cancer Research and Treatments
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- MicroRNA in disease regulation
Peking University
2016-2025
Peking University Third Hospital
2016-2025
Tsinghua University
2010-2025
Soochow University
2008-2025
First Affiliated Hospital of Soochow University
2009-2025
Center for Life Sciences
2015-2025
Zhejiang Gongshang University
2025
The First Affiliated Hospital, Sun Yat-sen University
2024-2025
Sun Yat-sen University
2024-2025
Key Laboratory of Guangdong Province
2024-2025
Abstract Epigenetic alteration of tumor suppression gene is one the most significant indicators in human esophageal squamous cell carcinoma (ESCC). In this study, we identified a novel ESCC hypermethylation biomarker ZNF132 by integrative computational analysis to comprehensive genome-wide DNA methylation microarray dataset. We validated status 91 Chinese Han patients and adjacent normal tissues with target bisulfite sequencing (MTBS) assay. Meanwhile, silencing mediated was confirmed both...
ABSTRACT Interferons (IFNs) are key mediators of the host innate antiviral immune response. To identify IFN-stimulated genes (ISGs) that instigate an state against two medically important flaviviruses, West Nile virus (WNV) and dengue (DENV), we tested 36 ISGs commonly induced by IFN-α for activity viruses. We discovered five efficiently suppressed WNV and/or DENV infection when they were individually expressed in HEK293 cells. Mechanistic analyses revealed structurally related cell plasma...
Covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) is formed by conversion capsid-associated relaxed (rcDNA) via unknown mechanisms and exists in the nucleus infected hepatocyte as a minichromosome that serves transcription template for viral RNAs. To study molecular pathway cccDNA formation its regulation cellular factors, we have established cell line supports replication an envelope protein-deficient HBV genome tetracycline-inducible manner. Following induction...
ABSTRACT Tetherin and IFITM3 are recently identified interferon-induced cellular proteins that restrict infections by retroviruses filoviruses of influenza virus flaviviruses, respectively. In our efforts to further explore their antiviral activities against other viruses determine mechanisms, we found the two potently inhibit infection vesicular stomatitis (VSV), a prototype member Rhabdoviridae family. Taking advantage this well-studied system, show although both tetherin plasma membrane...
Current approaches to fabrication of nSC composites for bone tissue engineering (BTE) have limited capacity achieve uniform surface functionalization while replicating the complex architecture and bioactivity native bone, compromising application these nanocomposites in situ regeneration. A robust biosilicification strategy is reported impart a stable osteoinductive porous collagen scaffolds. The resultant possess native-bone-like structure nanosilica coating. osteoinductivity scaffolds...
Osteoarthritis (OA) is a progressive joint disease characterized by inflammation and cartilage destruction, its progression closely related to imbalances in the M1/M2 synovial macrophages. A two-pronged strategy for regulation of intracellular/extracellular nitric oxide (NO) hydrogen protons reprogramming macrophages proposed. The combination carbonic anhydrase IX (CA9) siRNA NO scavenger "two-in-one" nanocarriers (NAHA-CaP/siRNA nanoparticles) developed OA therapy scavenging inhibiting CA9...
The regeneration of osteochondral tissue necessitates the re-establishment a gradient owing to unique characteristics and healing potential chondral osseous phases. As self-healing capacity hyaline cartilage is limited, timely mechanical support during neo-cartilage formation crucial achieving optimal repair efficacy. In this study, we devised biodegradable bilayered scaffold, comprising chondroitin sulfate (CS) hydrogel regenerate porous pure zinc (Zn) scaffold for underlying bone as layer....
ABSTRACT Hepatitis C virus (HCV) infection is a common cause of chronic hepatitis and currently treated with alpha interferon (IFN-α)-based therapies. However, the underlying mechanism IFN-α therapy remains to be elucidated. To identify cellular proteins that mediate antiviral effects IFN-α, we created HEK293-based cell culture system inducibly express individual interferon-stimulated genes (ISGs) determined their against HCV. By screening 29 ISGs are induced in Huh7 cells by and/or...
The liver-specific microRNA miR-122 has been shown to be required for the replication of hepatitis C virus (HCV) in hepatoma cell line Huh7. aim this study was test if HCV can modulated by exogenously expressed human embryonic kidney epithelial cells (HEK-293). Our results demonstrate that enhances colony formation efficiency replicon and increases steady-state level RNA HEK-293 cells. Therefore, we conclude although is not absolutely required, it greatly nonhepatic
Total meniscectomy leads to knee osteoarthritis in the long term. The poly(ε-caprolactone) (PCL) scaffold is a promising material for meniscal tissue regeneration, but cell-free scaffolds result relatively poor regeneration and lead joint degeneration.A novel, 3-dimensional (3D)-printed PCL augmented with mesenchymal stem cells (MSCs) would offer benefits cartilage protection.Controlled laboratory study.PCL were 3D printed seeded bone marrow-derived MSCs. Seventy-two New Zealand White...
Mitochondria form networks. Formation of mitochondrial networks is important for maintaining DNA integrity and interchanging material, whereas disruption the network affects functions. According to current view, are formed by fusion individual mitochondria. Here, we report a new mechanism formation through KIF5B-mediated dynamic tubulation We found that KIF5B pulls thin, highly tubules out Fusion these tubules, which mediated mitofusins, gives rise network. further demonstrated sufficient...
Fructose-1,6-bisphosphate (FBP) aldolase links sensing of declining glucose availability to AMPK activation via the lysosomal pathway. However, how transmits lack occupancy by FBP remains unclear. Here, we show that FBP-unoccupied interacts with and inhibits endoplasmic reticulum (ER)-localized transient receptor potential channel subfamily V, inhibiting calcium release in low glucose. The decrease at contact sites between ER lysosome renders inhibited TRPV accessible bind v-ATPase then...
Anisotropic meniscus engineered using biomechanical, biochemical, and structural cues demonstrates long-term knee chondroprotection in rabbits.
Osteoarthritis (OA) is a degenerative joint disease characterized by progressive erosion of the articular cartilage and inflammation. Mesenchymal stem cells' (MSCs) transplantation in OA treatment emerging, but its clinical application still limited low efficiency oriented differentiation. In our study, to improve therapeutic efficiencies MSCs carbonic anhydrase IX (CA9) siRNA (siCA9)-based inflammation regulation Kartogenin (KGN)-based chondrogenic differentiation, combination strategy...
Abstract The musculoskeletal system, which is responsible for weight‐bearing, movement, and organ protection, faces many disorders arising from injuries, diseases, trauma that affect millions of people worldwide, resulting in a decreased quality life socioeconomic burden. Tissue engineering at the forefront current research on tissue regeneration demonstrates great potential repair. Among numerous grafts available, adhesive hydrogels have demonstrated applications. Despite surge development...
Abstract Migrasomes are organelles that generated by migrating cells. Here we report the key role of neutrophil-derived migrasomes in haemostasis. We found a large number exist blood mice and humans. Compared with neutrophil cell bodies platelets, these adsorb enrich coagulation factors on surface. Moreover, they highly enriched adhesion molecules, which enable them to preferentially accumulate at sites injury, where trigger platelet activation clot formation. Depletion neutrophils, or...