A5100777524- RNA Research and Splicing
- MicroRNA in disease regulation
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Metabolomics and Mass Spectrometry Studies
- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- RNA regulation and disease
- Cancer Genomics and Diagnostics
- Diet and metabolism studies
- Glutathione Transferases and Polymorphisms
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Endoplasmic Reticulum Stress and Disease
- Single-cell and spatial transcriptomics
- Advanced Proteomics Techniques and Applications
- RNA and protein synthesis mechanisms
- Marine Bivalve and Aquaculture Studies
- COVID-19 Impact on Reproduction
- Gut microbiota and health
- Advanced Biosensing Techniques and Applications
- Seaweed-derived Bioactive Compounds
- Glycosylation and Glycoproteins Research
- Biochemical effects in animals
- Advanced Breast Cancer Therapies
University of Florida
2021-2024
University of Florida Health
2021-2024
Chinese PLA General Hospital
2023
University of Macau
2023
Nantong Maternity and Child Health Hospital
2023
Nantong University
2023
Florida College
2021-2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2018-2022
University of Oulu
2022
Tsinghua University
2018-2022
An activatable graphene-based nanoprobe is used to develop a multicolor immunosorbent assay for the detection of tumor protein biomarkers.
Echinoderms exhibit several fascinating evolutionary innovations that are rarely seen in the animal kingdom, but how these animals attained such features is not well understood. Here we report sequencing and analysis of genome extensive transcriptomes sea cucumber
Abstract The recently developed 2b-restriction site-associated DNA (2b-RAD) sequencing method provides a cost-effective and flexible genotyping platform for aquaculture species lacking sufficient genomic resources. Here, we evaluated the performance of this in selection (GS) Yesso scallop ( Patinopecten yessoensis ) through simulation real data analyses using six statistical models. Our analysis revealed that prediction accuracies obtained 2b-RAD markers were slightly lower than those all...
Binding of microRNAs (miRNAs) to mRNAs normally results in post-transcriptional repression gene expression. However, extensive base-pairing between miRNAs and target RNAs can trigger miRNA degradation, a phenomenon called RNA-directed degradation (TDMD). Here, we systematically analyzed Argonaute-CLASH (cross-linking, ligation, sequencing miRNA–target RNA hybrids) data identified numerous candidate TDMD triggers, focusing on their ability induce nontemplated nucleotide addition at the 3′...
Abstract MicroRNAs (miRNA) load onto AGO proteins to target mRNAs for translational repression or degradation. However, miRNA degradation can be triggered when extensively base-paired with RNAs, which induces confirmational change of and recruitment ZSWIM8 ubiquitin ligase mark proteasomal This RNA-directed (TDMD) mechanism appears evolutionarily conserved, but recent studies have focused on mammalian systems. Here, we performed AGO1-CLASH in Drosophila S2 cells, Dora (ortholog vertebrate...
Abstract MicroRNAs (miRNAs) are small non‐coding RNAs that play a fundamental role in enabling miRNA‐mediated target repression, post‐transcriptional gene regulatory mechanism preserved across metazoans. Loss of certain animal miRNA genes can lead to developmental abnormalities, disease, and various degrees embryonic lethality. These short normally guide Argonaute (AGO) proteins RNAs, which turn translationally repressed destabilized, silencing the fine‐tune expression maintain cellular...
Abstract While linear ubiquitin plays critical roles in multiple cell signaling pathways, few substrates have been identified. Global profiling of represents a significant challenge because the low endogenous level ubiquitination and background interference arising from highly abundant linkages (e.g. K48- K63-) non-specific attachment interfering proteins to polyubiquitin chain. We developed bio-orthogonal probe by site-specific encoding norbornene amino acid on (NAEK-Ub). This facilitates...
MicroRNAs (miRNA) are short non-coding RNAs widely implicated in gene regulation. Most metazoan miRNAs utilize the RNase III enzymes Drosha and Dicer for biogenesis. One notable exception is RNA polymerase II transcription start sites (TSS) whose biogenesis does not require Drosha. The functional importance of TSS-miRNA uncertain. To better understand function TSS-miRNAs, we applied a modified C rosslinking, L igation, nd S equencing H ybrids on Argonaute (AGO-qCLASH) to identify targets...
Chronic inflammation-induced oxidative stress is an important driving force for developing colitis-associated cancer (CAC). 4-hydroxynonenal (4-HNE) a highly reactive aldehyde derived from lipid peroxidation of ω-6 polyunsaturated fatty acids that contributes to colorectal carcinogenesis. Glutathione S-transferase alpha 4 (Gsta4) specifically conjugates glutathione 4-HNE and thereby detoxifies 4-HNE. The correlation these biomarkers with the pathological changes in CAC is, however, unclear....
A systematic comparison is presented for the effects of seven different normalization schemes in quantitative urinary metabolomics. Morning spot urine samples were analyzed with nuclear magnetic resonance (NMR) spectroscopy from a population-based group 994 individuals. Forty-four metabolites quantified and metabolite–metabolite associations metabolite concentrations two representative clinical measures, body mass index mean arterial pressure, analyzed. Distinct differences observed when...
Summary Since its establishment in 2009, single-cell RNA-seq has been a major driver behind progress biomedical research. In developmental biology and stem cell studies, the ability to profile single cells confers particular benefits. While most studies still focus on individual tissues or organs, recent development of ultra-high-throughput demonstrated potential power characterizing more complex systems even entire body. However, although multiple have attracted attention, no systematic...
Hyperlipidemia is one of the main causes obesity, type 2 diabetes mellitus (T2DM), and atherosclerosis. The adenosine derivative, 2?,3?,5?-triacetyl-N6-(3-hydroxylaniline) (IMM-H007) an effective lipid-lowering compound that has important implications for development drugs. Metabolomic analysis based on 1H NMR was used to monitor dynamic changes in diverse biological media including serum, liver, urine, feces response high-fat diet (HFD) IMM-H007 treatments. Ultraperformance liquid...
Abstract Diseases such as cancer arise from systematical reconfiguration of interactions exceedingly large numbers proteins in cell signaling. The study complicated molecular mechanisms requires multiplexed detection the inter-connected activities several a disease-associated context. However, existing methods are generally not well-equipped for this kind application. Here method analyzing functionally linked protein is developed based on enzyme controlled pairing between complementary...
Introduction: Aberrations in cell cycle control is defined as one of the hallmarks cancer, while cyclin D1 an essential protein to which promote G1 phase into S phase, and frequently overexpressed many human cancers.However, new functions have been identified transgenic mice models, including transcription genome, development chromosome instability DNA repair.In this research, our aim find function cancers.Methods: The correlation expression levels prognosis cervical cancer patients were...
While much is known about miRNA biogenesis and canonical targeting, relatively less understood decay. The major decay pathway in metazoans mediated through target-directed degradation (TDMD), which certain RNAs can 'trigger' All triggers for TDMD base pair with the seed, extensively on 3 prime end, a pattern that supposedly induces TDMD-competent conformational change of Argonaute (Ago), allowing turnover. Here, we utilized Ago1-CLASH to find Drosophila transcript Kah contains at least two...
ABSTRACT MicroRNAs (miRNA) are short non-coding RNAs widely implicated in gene regulation. Most metazoan miRNAs utilize the RNase III enzymes Drosha and Dicer for biogenesis. One notable exception is RNA polymerase II transcription start sites (TSS) whose biogenesis does not require Drosha. The functional importance of TSS-miRNA uncertain. To better understand function TSS-miRNAs, we applied a modified C rosslinking, L igation, S equencing H ybrids on Argonaute (AGO-qCLASH) to identify...
A total synthesis approach of CS-E oligosaccharides was established and a series derivatives were synthesized. These evaluated for glycosaminoglycan (GAG)-binding protein interaction against cytokines, midkine, pleiotrophin, by surface-plasmon resonance (SPR) assay. The binding epitopes to midkine mapped using saturation transfer difference (STD) NMR technique. groups on the reducing end contributed affinity, should not be ignored in biological assays. findings contribute structure activity...