BACKGROUND Verapamil-sensitive, idiopathic left ventricular tachycardia (ILVT) with right bundle branch block configuration and left-axis deviation has been suggested to originate from the posterior fascicle. The purpose of this study was determine how frequently potentials generated by Purkinje fiber network (P potential) can be recorded preceding activation, role P potential in guiding radiofrequency catheter ablation. METHODS AND RESULTS Eight patients (mean age, 26 +/- 10 years) ILVT...

This study examined the moderator effect of social support on relationship between stress and depression university students. A total 632 undergraduate students completed measures perceived stress, support, depression. Hierarchical regression analysis showed that moderated association Undergraduate with high reported higher scores in than those low level. However, impact was much smaller group compared group.

Accessory pathways (APs) exhibiting "Mahaim fiber" physiology (antegrade conduction only, long time, and decremental properties) often connect the lateral right atrium to bundle branch (right atriofascicular pathways). Potentials from these have not been recorded previously. The purpose of this study was determine whether AP activation potentials could be APs used localize a site for catheter ablation.Of 26 consecutive patients referred ablation an producing preexcited (antidromic)...

Abstract Intestinal commensals are potential important contributors to the etiology of sporadic colorectal cancer, but mechanisms by which bacteria can initiate tumors remain uncertain. Herein, we describe that link Enterococcus faecalis, a bacterium known produce extracellular superoxide, acute induction chromosomal instability. Immortalized human and nontransformed murine colonic epithelial cells, along with mouse ligation model, were used assess effect E. faecalis on genomic DNA stability...

<h3>Objective</h3> Commensal bacteria and innate immunity play a major role in the development of colorectal cancer (CRC). We propose that selected commensals polarise colon macrophages to produce endogenous mutagens initiate chromosomal instability (CIN), lead expression progenitor tumour stem cell markers, drive CRC through bystander effect. <h3>Design</h3> Primary murine epithelial cells were repetitively exposed <i>Enterococcus faecalis</i>-infected macrophages, or purified...

Enterococcus faecalis-infected macrophages produce 4-hydroxynonenal (4-HNE) that mediates microbiota-induced bystander effect (MIBE) leading to colorectal cancer (CRC). Glutathione S-transferase alpha 4 (Gsta4), a specific detoxifying enzyme for 4-HNE, is overexpressed in human CRC and E. faecalis-induced murine CRC. However, the roles of Gsta4 colitis remain unclear. Herein, we demonstrate essential MIBE by protecting from ferroptosis. faecalis OG1RFSS was used induce Gsta4-/-...

Intestinal commensal bacteria have recently been shown to trigger macrophages produce diffusible clastogens (or chromosome-breaking factors) through a bystander effect (BSE) that mediates DNA damage and induces chromosomal instability in neighboring cells. Colon appear central colon carcinogenesis BSE the expression of tumor necrosis factor-α (TNF-α) cyclooxygenase-2 (COX-2). The former netrin-1, regulator intestinal epithelial cell apoptosis, latter generates trans-4-hydroxy-2-nonenal...

Objectives. To ascertain if mitochondrial dysfunction (MD) of kidney cells is present in severe hemorrhagic shock and to investigate whether polydatin (PD) can attenuate MD its protective mechanisms. Research Design Methods. Renal tubular epithelial (RTECs) from rat kidneys experiencing HS a cell line (HK‐2) under hypoxia/reoxygenation (H/R) treatment were used. Morphology function mitochondria isolated RTECs or cultured HK‐2 evaluated, accompanied by apoptosis pathway‐related proteins....

Abstract Background Apolipoprotein (Apo) may be associated with type 2 diabetes (T2D), however, little is known whether or not serum apolipoproteins are correlated fasting blood glucose (FBG) and the prevalence of T2D in Chinese populations. In this study, we examined association ApoA1, ApoB, ratio ApoB/ApoA1 (ApoB/A1 ratio) FBG level, compared apolipoprotein indicators predicting adults. Methods A total 1027 subjects were enrolled cross-sectional study. The ApoB/A1 was determined using...

The aim of the study was find out whether neuronal mitochondrial injury does take place in severe shock and to explore effective therapy for shock.Rats were divided following group: sham, + normal saline (NS), cyclosporine A (CsA), resveratrol (Res) polydatin (PD). Rats subjected 2 h, followed by administration NS, CsA, Res PD, infusion shed blood. Morphology, metabolism function mitochondria measured.Increased lipid peroxides (LPO) levels, lysosomal permeability transition pore opening took...

Mitochondrial injury is a major cause of sepsis-induced organ failure. Polydatin (PD), natural polyphenol, demonstrates protective mitochondrial effects in neurons and arteriolar smooth muscle cells during severe shock. In this study, we investigated the PD on renal tubular epithelial cell (RTEC) mitochondria rat model acute kidney injury.Rats underwent cecal ligation puncture (CLP) to mimic injury. Rats were randomly divided into sham, CLP + normal saline, vehicle, groups. Normal 30 mg/kg...

Objective: The aim of the study was to determine whether hepatocyte mitochondrial injury instigates severe shock and explore effective therapy.Methods: Wistar rats were randomly divided into five groups: Control (sham) group, + normal saline, cyclosporine A, resveratrol (Res) polydatin (PD) group. Mitochondrial morphology function in hepatocytes following treatment determined.Results: Hepatocytes exhibited dysfunction characterized with opening permeability transition pores, swelling,...

// Xingmin Wang 1, 2 , Yonghong Yang 3, 4 and Mark M. Huycke 2, 5 1 Department of Radiation Oncology, University Oklahoma Health Sciences Center, City, OK 73104, USA The Muchmore Laboratories for Infectious Diseases Research, City VA Care System, 3 Gansu Province Children's Hospital, Lanzhou, 730030, China Key Laboratory Gastrointestinal Cancer, Lanzhou Second Internal Medicine, 73126, Correspondence to: Wang, email: xingmin-wang@ouhsc.edu Huycke, mark-huycke@ouhsc.edu Keywords: macrophage;...

Abstract Infection of macrophages by the human intestinal commensal Enterococcus faecalis generates DNA damage and chromosomal instability in mammalian cells through bystander effects. These effects are characterized clastogenesis to mitotic spindles target mediated, part, trans-4-hydroxy-2-nonenal (4-HNE). In this study, we investigated role COX lipoxygenase (LOX) producing reactive aldehyde using E. faecalis–infected interleukin (IL)-10–knockout mice colonized with commensal. 4-HNE...

Sepsis is a systemic inflammatory disease caused by multiple pathogens, with the most commonly affected organ being lung. 3-Hydroxybutyrate plays protective role in diseases through autophagy promotion; however, exact mechanism remains unexplored.Our study used MIMIC-III database to construct cohort of ICU sepsis patients and figure out correlation between level ketone bodies clinical prognosis septic patients. In vivo vitro models were reveal 3-hydroxybutyrate sepsis-associated acute lung...

The main objective of this study was to investigate the activity polydatin on mitochondrial dysfunction and lysosomal stability arteriolar smooth muscle cells (ASMCs) in severe shock. experimental animals (rats) were divided into five groups: control, hemorrhagic shock, shock + CsA, Res, PD (exposed cyclosporin A, resveratrol, or following induction respectively). calcein-Co(2+) technique revealed opening ASMC permeability transition pores (mPTP) after with resulting swelling, decreased...

ABSTRACT Type E botulinum toxin (BoNT/E)-producing Clostridium butyricum strains isolated from botulism cases or soil specimens in Italy and China were analyzed by using nucleotide sequencing of the bont /E gene, random amplified polymorphic DNA (RAPD) assay, pulsed-field gel electrophoresis (PFGE), Southern blot hybridization for gene. Nucleotide sequences genes 11 Chinese isolates Italian strain BL 6340 determined. The C. identical. deduced amino acid sequence BoNT/E showed 95.0 96.9%...