A5073309578- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- interferon and immune responses
- COVID-19 Clinical Research Studies
- NF-κB Signaling Pathways
- Animal Virus Infections Studies
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Plant Reproductive Biology
- Monoclonal and Polyclonal Antibodies Research
- RNA regulation and disease
- Long-Term Effects of COVID-19
- Plant nutrient uptake and metabolism
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- vaccines and immunoinformatics approaches
- Cytokine Signaling Pathways and Interactions
- Evolution and Genetic Dynamics
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- SARS-CoV-2 detection and testing
Capital Medical University
1970-2025
Beijing Ditan Hospital
1970-2025
National Institute for Viral Disease Control and Prevention
2025
Sun Yat-sen University
2024
Shanxi Agricultural University
2024
Hospital of Stomatology, Sun Yat-sen University
2024
South China Normal University
2024
National Marine Environmental Forecasting Center
1970-2023
National Institute for Communicable Disease Control and Prevention
1970-2023
Jiangnan University
2022
SARS-CoV-2 uses human ACE2 as a primary receptor for host cell entry. Viral entry mediated by the interaction of with spike protein largely determines range and is major constraint to interspecies transmission. We examined activity 14 orthologs found that wild-type mutant lacking furin cleavage site in S could utilize from broad animal species enter cells. These results have important implications natural hosts, transmission, models, molecular basis binding SARS-CoV-2.
Virus entry into host cells is one of the key determinants range and cell tropism subjected to control innate adaptive immune responses. In last decade, several interferon-inducible cellular proteins, including IFITMs, GILT, ADAP2, 25CH, LY6E, had been identified modulate infectious a variety viruses. Particularly, LY6E was recently as factor that facilitates human-pathogenic viruses, human immunodeficiency virus, influenza A yellow fever virus. Identification potent restriction...
The administration of COVID-19 vaccines is the primary strategy used to prevent further infections by COVID-19, especially in people living with HIV (PLWH), who are at increased risk for severe symptoms and mortality. However, vaccine hesitancy, safety, immunogenicity among PLWH have not been fully characterized. We estimated hesitancy status vaccination Chinese PLWH, explored safety impact on antiviral therapy (ART) efficacy compared an inactivated between healthy controls (HC). In total,...
Diverse SARS-like coronaviruses (SL-CoVs) have been identified from bats and other animal species. Like SARS-CoV, some bat SL-CoVs, such as WIV1, also use angiotensin converting enzyme 2 (ACE2) human entry receptor. However, whether these viruses can the ACE2 of species their receptor remains to be determined. We report herein that WIV1 has a broader tropism orthologs than SARS-CoV isolate Tor2. Among 9 examined, exhibited highest efficiency mediate infection pseudotyped virus. Our findings...
Spontaneous mutations introduce uncertainty into coronavirus disease 2019 (COVID-19) control procedures and vaccine development. Here, we perform a spatiotemporal analysis on intra-host single-nucleotide variants (iSNVs) in 402 clinical samples from 170 affected individuals, which reveals an increase genetic diversity over time after symptom onset individuals. Nonsynonymous are overrepresented the pool of iSNVs but underrepresented at polymorphism (SNP) level, suggesting two-step fitness...
After a short recovery period, COVID-19 reinfections could occur in convalescent patients, even those with measurable levels of neutralizing antibodies. Effective vaccinations and protective public health measures are recommended for the patients.
Background/Objectives: Developing durable cellular immunity remains a critical challenge for HIV vaccine development. Methods: We evaluated sequential and repeated heterologous prime–boost vaccination regimen using four distinct vector-based vaccines (DNA, rAd5, rSeV, rMVA) expressing HIV-1 gag in rhesus macaques over decade-long observation period. Results: Compared to the two-vector control groups, four-vector elicited potent gag-specific immune responses, as evidenced by IFN-γ ELISPOT...
IFN regulatory factor-3 is a transcription factor that required for the rapid induction of type I IFNs in innate antiviral response. Two noncanonical IkappaB kinase (IKK) family members, IKKepsilon and TRAF family-associated NF-kappaB activator-binding kinase-1, have been shown to phosphorylate are critically involved virus-triggered TLR3-mediated signaling leading IFNs. In yeast two-hybrid screens potential IKKepsilon-interacting proteins, we identified Ret finger protein (RFP) as an...
Abstract Host cells orchestrate the production of IFN-β upon detecting invading viral pathogens. Here, we report that Ring finger protein 166 (RNF166) potentiates RNA virus-triggered production. Overexpression RNF166 rather than its homologous proteins RNF114, RNF125 and RNF138, enhanced Sendai virus (SeV)-induced activation promoter. Knockdown endogenous RNF166, but not other RNFs, inhibited induced by SeV encephalomyocarditis virus. interacted with TRAF3 TRAF6. SeV-induced ubiquitination...
Retinoic acid-inducible gene I (RIG-I) is a key sensor for recognizing nucleic acids derived from RNA viruses and triggers beta interferon (IFN-β) production. Because of its important role in antiviral innate immunity, the activity RIG-I must be tightly controlled. Here, we used yeast two-hybrid screening to identify SEC14 family member, SEC14L1, as RIG-I-associated negative regulator. Transfected SEC14L1 interacted with RIG-I, endogenous associated viral infection-inducible manner....
ABSTRACT The COVID-19 pandemic has caused an unprecedented global public health and economy crisis. origin emergence of its causal agent, SARS-CoV-2, in the human population remains mysterious, although bat pangolin were proposed to be natural reservoirs. Strikingly, comparing SARS-CoV-2-like CoVs identified bats pangolins, SARS-CoV-2 harbors a polybasic furin cleavage site spike (S) glycoprotein. uses ACE2 as receptor infect cells. Receptor recognition by S protein is major determinant host...
Interferons (IFNs) control viral infections by inducing expression of IFN-stimulated genes (ISGs) that restrict distinct steps replication. We report herein gamma-interferon-inducible lysosomal thiol reductase (GILT), a lysosome-associated ISG, restricts the infectious entry selected enveloped RNA viruses. Specifically, we demonstrated GILT was constitutively expressed in lung epithelial cells and fibroblasts its could be further induced type II interferon. While overexpression inhibited...
The yellow fever virus (YFV) recently reemerged in the large outbreaks Africa and Brazil, first imported patients into Asia have recalled concerns of YFV evolution. Here we show phylogenomics with serial clinical samples 2016 infections. Phylogenetics exhibited that strains were close to Angola 1971 only three amino acid changes presented new other lineages. Deep sequencing viral genomes discovered 101 intrahost single nucleotide variations (iSNVs) 234 polymorphisms (SNPs). Analysis iSNV...
Abstract Objectives To describe a high-sensitivity SARS-CoV-2 antigen test that is based on the fully automated light-initiated chemiluminescent immunoassay (LiCA ® ), and to validate its analytical characteristics clinical agreement detecting infection against reference molecular test. Methods Analytical performance was validated detection limits were determined using different types of nucleocapsid protein samples. 798-pair anterior nasal swab specimens collected from hospitalized patients...
Canine coronavirus-human pneumonia-2018 (CCoV-HuPn-2018) was recently isolated from a child with pneumonia. This novel human pathogen resulted cross-species transmission of canine coronavirus. It has been known that CCoV-HuPn-2018 uses aminopeptidase N (APN) canines, felines, and porcines, but not humans, as functional receptors for cell entry. The molecular mechanism entry in remains poorly understood. In this study, we demonstrated among the nine APN orthologs tested, Mexican free-tailed...
ABSTRACT C3A is a sub-clone of human hepatoblastoma HepG2 cell line with the strong contact inhibition growth. We fortuitously found that was more susceptible to coronavirus HCoV-OC43 infection than HepG2, which attributed increased efficiency virus entry into cells. In an effort search for host cellular protein(s) mediating differential susceptibility two lines infection, we ADAP2, GILT and LY6E, three proteins known activity interfering entry, expressed at significantly higher levels in...
Transmitted drug resistance (TDR) is a critical ongoing public health challenge in HIV/AIDS therapy. We explore the prevalence of TDR, its patterns, associated risk factors, and predicted sensitivity Beijing between 2015 2018.Retrospective data on TDR from 3265 antiretroviral therapy (ART)-naïve patients were collected at Ditan Hospital 1 August 2014 to 31 July 2018. was defined according Stanford Drug Resistance Mutations Database. prevalence, pattern, analysed.The overall HIV-1 6.68% (218...