A5086082140- Hepatitis B Virus Studies
- Hepatitis C virus research
- Chemical Synthesis and Analysis
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Diatoms and Algae Research
- Supramolecular Self-Assembly in Materials
- Enzyme Structure and Function
- Poxvirus research and outbreaks
- RNA and protein synthesis mechanisms
- HIV/AIDS drug development and treatment
- Herpesvirus Infections and Treatments
- Computational Drug Discovery Methods
- interferon and immune responses
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Molecular Junctions and Nanostructures
- HIV Research and Treatment
- Sirtuins and Resveratrol in Medicine
- DNA and Nucleic Acid Chemistry
- Mosquito-borne diseases and control
- Protein purification and stability
- Click Chemistry and Applications
- Calcium Carbonate Crystallization and Inhibition
- Virology and Viral Diseases
Baruch S. Blumberg Institute
2015-2025
TherimuneX Pharmaceuticals (United States)
2024
Heifer International
2022
Fox Chase Chemical Diversity Center
2020
United States Naval Research Laboratory
2007-2018
Hepatitis B Foundation
2016-2017
New York University
2004-2014
Jackson State University
2010
Cornell University
2008
Japanese Foundation For Cancer Research
2005-2007
The nanoscale morphology and protein secondary structure of barnacle adhesive plaques were characterized using atomic force microscopy (AFM), far-UV circular dichroism (CD) spectroscopy, transmission Fourier transform infrared (FTIR) Thioflavin T (ThT) staining. Both primary cement (original laid down by the barnacle) (cement used for reattachment) from Balanus amphitrite (= Amphibalanus amphitrite) analyzed. Results showed that both cements consisted largely nanofibrillar matrices having...
We previously reported the design and synthesis of a new class artificial α-helices in which an N-terminal main-chain hydrogen bond is replaced by carbon−carbon derived from ring-closing metathesis reaction [Chapman, R. N.; Dimartino, G.; Arora, P. S. J. Am. Chem. Soc. 2004, 126, 12252−12253]. Our initial study utilized alanine-rich sequence; present manuscript we evaluate potential this method for very short (10 residues) representing two different biologically relevant α-helical domains....
ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTAffinity Selection of Peptide Phage Libraries against Single-Wall Carbon Nanohorns Identifies a Aptamer with Conformational VariabilityDaisuke Kase, John L. Kulp, Masako Yudasaka, Spencer Evans, Sumio Iijima, and Kiyotaka ShibaView Author Information Department Protein Engineering, Cancer Institute, Japanese Foundation for Research, Toshima, Tokyo 170-8455, Japan, Laboratory Chemical Physics, New York University, York, 10010, NEC, Miyukigaoka, Tsukuba...
The understanding of biomineralization and realization biology-inspired materials technologies depends on the nature chemical physical interactions between proteins biominerals or synthetically made inorganic materials. Recently, combinatorial genetic techniques permit isolation peptides recognizing specific that are used as molecular building blocks for novel applications. Little is known about structure these recognition mechanisms onto their counterpart surfaces. Here, we report...
Stimulator of interferon genes (STING) is an integral ER-membrane protein that can be activated by 2'3'-cGAMP synthesized cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) upon binding double-stranded DNA. It activates (IFN) and inflammatory cytokine responses to defend against infection microorganisms. Pharmacologic activation STING has been demonstrated induce antiviral state boost antitumor immunity. We previously reported a cell-based high-throughput-screening assay...
ABSTRACT Although a highly effective vaccine is available, the number of yellow fever cases has increased over past 2 decades, which highlights pressing need for antiviral therapeutics. In high-throughput screening campaign, we identified an acetic acid benzodiazepine (BDAA) compound potently inhibits virus (YFV). Interestingly, while treatment YFV-infected cultures with μM BDAA reduced virion production by greater than logs, was not active against 21 other viruses from 14 different viral...
Osteoporosis is a major public health problem. Currently, there are no orally available therapies that increase bone formation. Intermittent parathyroid hormone (PTH) stimulates formation through signal transduction pathway involves inhibition of salt-inducible kinase isoforms 2 and 3 (SIK2 SIK3). Here, we further validate SIK2/SIK3 as osteoporosis drug targets by demonstrating ubiquitous deletion these genes in adult mice increases without extraskeletal toxicities. Previous efforts to...
Capsid assembly modulators (CAMs) are a novel class of antiviral agents in clinical development for the treatment chronic hepatitis B. CAMs inhibit B virus (HBV) replication by binding to hydrophobic pocket, i.e., HAP between HBV capsid protein (Cp) dimer–dimer interfaces misdirect its into empty capsids or aberrant structures and designated as CAM-E CAM-A, respectively. Because emergence CAM-resistant variants results failure therapy, it is important rationally design with high barrier...
Chronic hepatitis B virus (HBV) infection is a global public health problem. Although the currently approved medications can reliably reduce viral load and prevent progression of liver diseases, they fail to cure infection. In an effort toward discovery novel antiviral agents against HBV, group benzamide (BA) derivatives that significantly reduced amount cytoplasmic HBV DNA were discovered. The initial lead optimization efforts identified two BA with improved activity for further mechanistic...
Hepatitis B virus (HBV) core protein is a small with 183 amino acid residues and assembles the pregenomic (pg) RNA viral DNA polymerase to form nucleocapsids. During last decades, several groups have reported HBV allosteric modulators (CpAMs) distinct chemical structures. CpAMs bind hydrophobic HAP pocket located at dimer–dimer interface induce conformational changes in subunits. While Type I CpAMs, heteroaryldihydropyrimidine (HAP) derivatives, misdirect dimers assemble noncapsid polymers,...
Most drugs used to treat viral disease target a virus-coded product. They inhibit single virus or family, and the pathogen can readily evolve resistance. Host-targeted antivirals overcome these limitations. The broad-spectrum activity achieved by host targeting be especially useful in combating emerging viruses for treatment of diseases caused multiple pathogens, such as opportunistic agents immunosuppressed patients. We have developed family compounds that modulate sirtuin 2, an...
Simulated annealing of chemical potential located the highest affinity positions eight organic probes and water on static structures hen egg white lysozyme (HEWL) in various conformational states. In all HELW conformations, a diverse set clustered known binding site (hot spot). Fragment clusters at other locations were excluded by tightly-bound waters so that only hot-spot cluster remained each case. The location hot spot was correctly predicted irrespective protein conformation without...
Adsorption behavior of a gold binding peptide was experimentally studied to achieve kinetics and thermodynamics parameters toward understanding the an engineered onto solid metal surface. The gold-binding peptide, GBP1, originally selected using cell surface display library contains 14 amino acid residues. In this work, single- three-repeats GBP1 were used assess effects two parameters: molecular architecture versus secondary structure on adsorption substrate. measurements carried out...
The gold crystal-forming E. coli polypeptide sequence, MHGKTQATSGTIQS, is one of several sequences that interacts with interfaces and catalyzes the formation Au crystals in solution, nucleated preferentially featuring (111) interface. To date, there have been no experimental studies which explore structure coli-expressed binding proteins or Au(III) ions by these polypeptides. In this present report, multidisciplinary approaches were applied to 42-AA protein-1 (GBP-1/42) a model representing...
We report the design, synthesis, and characterization of a short peptide trapped in π-helix configuration. This high-energy conformation was nucleated by preorganized π-turn, which obtained replacing an N-terminal intramolecular main chain i + 5 hydrogen bond with carbon−carbon bond. Our studies highlight nucleation parameter as key factor contributing to relative instability allow us estimate fundamental helix−coil transition parameters for this conformation.
// Aejaz Sayeed 1 , Brielle E. Dalvano David Kaplan 2 3 Usha Viswanathan John Kulp Alhaji H. Janneh 4 Lu-Yu Hwang 5 Adam Ertel 6 Cataldo Doria 7 and Timothy Block Baruch S. Blumberg Institute, Doylestown, PA, USA University of Pennsylvania Perelman School Medicine, Philadelphia, The Corporal Michael J. Crescenz Veterans Administration Hospital, Current address: Department Biochemistry Molecular Biology, Medical South Carolina, Charleston, SC, Epidemiology, Human Genetics Environment Science...
Abstract Organisms have evolved biomaterials with an extraordinary convergence of high mechanical strength, toughness, and elasticity. In contrast, synthetic materials excel in stiffness or extensibility, a combination the two is necessary to exceed performance natural biomaterials. We bridge this property gap through side-chain-to-side-chain polymerization cyclic β-peptide rings. Due their strong dipole moments, rings self-assemble into rigid nanorods, stabilized by hydrogen bonds....
PCSK9 is a protein secreted by the liver that binds to low-density lipoprotein receptor (LDLR), causing LDLR internalization, decreasing clearance of circulating LDL particles. Mutations in strengthen its interactions with result familial hypercholesterolemia (FH) and early onset atherosclerosis, while nonsense mutations cardio-protective hypocholesterolemia. These observations led inhibition for cholesterol lowering becoming high-interest therapeutic target, antibody drugs reaching market....
Single-walled carbon nanohorns (SWNHs) are interesting nanostructures that have applications to science and technology. Using M13 phage display technology, polypeptides directed again SWNHs surfaces been created for a number of nanotechnology pharmaceutical purposes, yet the molecular mechanism polypeptide sequence interaction binding is not known. Recently, we identified linear 12-AA pIII sequence, NH-12-5-2 (DYFSSPYYEQLF), binds with high affinity surfaces. To probe structure this tail...