A5045466674- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Mosquito-borne diseases and control
- Herpesvirus Infections and Treatments
- CRISPR and Genetic Engineering
- T-cell and B-cell Immunology
- HIV-related health complications and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Viral gastroenteritis research and epidemiology
- Cellular transport and secretion
- Immune Response and Inflammation
- Sex work and related issues
- Immunodeficiency and Autoimmune Disorders
- Immune responses and vaccinations
- Neuroscience and Neuropharmacology Research
- Adolescent Sexual and Reproductive Health
- Respiratory viral infections research
- Hepatitis B Virus Studies
- Reproductive System and Pregnancy
Institut Català d'Investigació Química
2025
University of North Carolina at Chapel Hill
2015-2024
University of Alabama at Birmingham
2024
Office of Infectious Diseases
2011-2023
Johns Hopkins University Center for AIDS Research
2012-2023
University of North Carolina Health Care
2014-2021
Colorado State University
2019
University of California, Davis
2019
Indiana University School of Medicine
2013-2017
Hospital Universitario Río Hortega
2014
Background Worldwide, vaginal transmission now accounts for more than half of newly acquired HIV-1 infections. Despite the urgency to develop and implement novel approaches capable preventing HIV transmission, this process has been hindered by lack adequate small animal models preclinical efficacy safety testing. Given importance route we investigated susceptibility humanized mice intravaginal infection. Methods Findings We show that female reproductive tract bone marrow–liver–thymus (BLT)...
Macrophages have long been considered to contribute HIV infection of the CNS; however, a recent study has contradicted this early work and suggests that myeloid cells are not an in vivo source virus production. Here, we addressed role macrophages by first analyzing monocytes isolated from viremic patients undergoing antiretroviral treatment. We were unable find viral DNA or outgrowth peripheral blood. To determine whether tissue productively infected, used 3 different but complementary...
Intrarectal infection between men who have sex with represents a predominant form of human immunodeficiency virus (HIV) transmission in developed countries. Currently there are no adequate small animal models that recapitulate intrarectal HIV transmission. Here we demonstrate lymphocytes generated situ from hematopoietic stem cells reconstitute the gastrointestinal tract humanized mice CD4+ T rendering them susceptible to after single inoculation results systemic depletion gut-associated...
Primary cultures of rat spermatogenic cells that did not bind to collagen matrices were able colonize and form mature spermatozoa when transferred testes recipient males. Up 73% the progeny from matings with males derived cells. Subsequently, two populations germ obtained by selection on laminin matrices. Both expressed cell marker, DAZL, but somatic vimentin. The bound represented ≈5% total population greatly enriched in ability a testis, suggesting an enrichment germ-line stem colonization...
Productive entry of human immunodeficiency virus type 1 (HIV-1) into a host cell is believed to proceed via fusion the viral envelope with cell's plasma membrane. Interestingly, majority HIV-1 particles that bind surface are taken up by endocytosis; however, this mode internalization generally does not result in infection. Presumably, remain trapped endocytic pathway and eventually degraded. Here, we demonstrate treatment cells various pharmacological agents known elevate pH endosomes...
Here we demonstrate that a combination of tenofovir, emtricitabine, and raltegravir effectively suppresses peripheral systemic HIV replication in humanized BLT mice. We also antiretroviral therapy (ART)-treated mice harbor latently infected resting human CD4+ T cells can be induced ex vivo to produce HIV. observed the levels present are within range those circulating patients undergoing suppressive ART. These results potential as an attractive model for testing efficacy novel eradication strategies.
Successful antiretroviral pre-exposure prophylaxis (PrEP) for mucosal and intravenous HIV-1 transmission could reduce new infections among targeted high-risk populations including discordant couples, injection drug users, women men who have sex with men. Targeted PrEP be particularly effective at slowing the spread of if a single combination were found to broadly protective across multiple routes transmission. Therefore, we designed our in vivo preclinical study systematically investigate...
Resident microbiota activate regulatory cells that modulate intestinal inflammation and promote maintain homeostasis. IL-10 is a key mediator of immune function. Our studies described the functional importance mechanisms by which gut specific microbial components influenced development IL-10-producing B cells. We used fecal transplant to germ-free (GF) Il10+/EGFP reporter Il10-/- mice demonstrate from pathogen-free primarily stimulated colon-specific T regulatory-1 in ex-GF mice. turn...
Recent iPrEx clinical trial results provided evidence that systemic preexposure prophylaxis (PrEP) with emtricitabine (FTC) and tenofovir disoproxil fumarate (TDF) can partially prevent rectal HIV transmission in humans. Similarly, we have previously demonstrated administration of the same FTC-TDF combination efficiently prevented humanized bone marrow/liver/thymus (BLT) mice. The CAPRISA 004 recently topical application could vaginal HIV-1 To further validate usefulness BLT mouse model for...
Here we report an ultra-long-acting tunable, biodegradable, and removable polymer-based delivery system that offers sustained drug for up to one year HIV treatment or prophylaxis. This robust formulation the ability integrate multiple drugs in a single injection, which is particularly important address potential resistance with monotherapy. Six antiretroviral were selected based on their solubility N-methyl-2-pyrrolidone relevance as combination therapy prevention. All released...