A5086932565- Protein Structure and Dynamics
- Enzyme Structure and Function
- Advanced NMR Techniques and Applications
- Electron Spin Resonance Studies
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- ATP Synthase and ATPases Research
- Signaling Pathways in Disease
- Chemical Synthesis and Analysis
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Mass Spectrometry Techniques and Applications
- Mitochondrial Function and Pathology
- Hemoglobin structure and function
- DNA and Nucleic Acid Chemistry
- Molecular spectroscopy and chirality
- Neuropeptides and Animal Physiology
- Advanced Electron Microscopy Techniques and Applications
- Supramolecular Self-Assembly in Materials
- Immune Response and Inflammation
- Peptidase Inhibition and Analysis
- Photosynthetic Processes and Mechanisms
- Biofield Effects and Biophysics
- Glycosylation and Glycoproteins Research
- Cholinesterase and Neurodegenerative Diseases
Cornell University
2013-2023
Weill Cornell Medicine
2018-2023
Teagasc - The Irish Agriculture and Food Development Authority
2018
Agri Food and Biosciences Institute
2018
Government of Nepal
2018
Scotland's Rural College
2018
University of Edinburgh
2018
University College Dublin
2018
Bangor University
2018
New York University
2008
Background and Purpose Cardiolipin plays an important role in mitochondrial respiration cardiolipin peroxidation is associated with age‐related diseases. Hydrophobic interactions between cytochrome c converts from electron carrier to a peroxidase. In addition peroxidation, this impedes flux inhibits ATP synthesis. SS ‐31 ( D ‐ A rg‐dimethyl T yr‐ L ys‐ P he‐ NH 2 ) selectively binds peroxidase activity. Here, we examined whether also protected the function of . Experimental Approach...
The basic leucine zipper domain of the yeast transcription factor GCN4 consists a C-terminal and an N-terminal DNA-binding region that achieves stable structure only after association with DNA. Backbone dynamics peptide encompassing bZip (residues 226–281) are described using NMR spectroscopy. 15N longitudinal relaxation rates, transverse {1H}-15N nuclear Overhauser effects were measured for backbone amide nitrogen atoms at 290 K, 300 310 K. data interpreted reduced spectral density mapping...
Analysis of the ratio transverse and longitudinal relaxation rates (R2/R1) is an approach commonly used for estimation overall correlation time identification chemical exchange in biological macromolecules. However, this analysis fails to distinguish between motional anisotropy. We describe a simple method identifying anisotropy using product, R1R2. In slow tumbling regime, R1R2 product results constant value that independent This provides rapidly estimating dissociating effects NMR...
Limited chemical shift dispersion represents a significant barrier to studying multistate equilibria of large membrane proteins by 19F NMR. We describe novel monofluoroethyl probe that dramatically increases the dispersion. The improved conformational sensitivity and line shape enable detection previously unresolved states in one-dimensional (1D) NMR spectra 134 kDa transporter. Changes populations these response ligand binding, mutations, temperature correlate with population changes...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructural characterization of monellin in the alcohol-denatured state by NMR: Evidence for .beta.-sheet to .alpha.-helix conversionPei Fan, Clay Bracken, and Jean BaumCite this: Biochemistry 1993, 32, 6, 1573–1582Publication Date (Print):February 1, 1993Publication History Published online1 May 2002Published inissue 1 February 1993https://pubs.acs.org/doi/10.1021/bi00057a023https://doi.org/10.1021/bi00057a023research-articleACS PublicationsRequest...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and Nuclear Magnetic Resonance Structure Determination of an .alpha.-Helical, Bicyclic, Lactam-Bridged HexapeptideClay Bracken, Jozsef Gulyas, John W. Taylor, Jean BaumCite this: J. Am. Chem. Soc. 1994, 116, 14, 6431–6432Publication Date (Print):July 1, 1994Publication History Published online1 May 2002Published inissue 1 July 1994https://pubs.acs.org/doi/10.1021/ja00093a052https://doi.org/10.1021/ja00093a052research-articleACS...
Highlights•CD3γε and CD3δε can individually bind to the TCR yield a two-sided binding model•CD3γε binds β subunit α subunit•The molecular basis of TCR-CD3 interactions is examined through NMR spectroscopy•The extracellular structure revealed using computational dockingSummaryAntigen recognition peptide-major histocompatibility complexes (pMHCs) by T cells, key step in initiating adaptive immune responses, performed cell receptor (TCR) bound CD3 heterodimers. However, biophysical transmission...
Volatile anesthetics have been shown to modify immune cell functions via several mechanisms, some of which only partially elucidated. We demonstrated that isoflurane inhibits primary leukocyte integrin lymphocyte function-associated antigen-1 (LFA-1) by binding the allosteric cavity critical for conformational activation its high-affinity form. It remains be determined whether inhibition LFA-1 can generalized other such as sevoflurane.The effects sevoflurane on ability bind counter-ligand,...
The molecular and structural basis of anesthetic interactions with conformations functionalities cell surface receptors remains to be elucidated. We have demonstrated that the widely used volatile isoflurane blocks activation-dependent conformational conversion integrin lymphocyte function associated antigen-1 (LFA-1), major leukocyte adhesion molecule, a high-affinity configuration. Perturbation LFA-1 activation by at clinically relevant concentrations leads inhibition T-cell target cells...
Post-translationally modified tau is the primary component of neurofibrillary tangles, a pathological hallmark Alzheimer's disease and other tauopathies. Post-translational modifications (PTMs) within microtubule (MT)-binding domain (MBD), which encompasses two hexapeptide motifs that act as critical nucleating regions for aggregation, can potentially modulate aggregation well interactions with MTs membranes. Here, we characterize effects recently discovered PTM, lysine succinylation, on...
De novo protein design enables systematic exploration of the relationship between amino acid sequences, conformations, and thermodynamics proteins. The polypeptide α2D is a de designed dimeric four-helix bundle with native-like three-dimensional structure [Hill, R. B.; DeGrado, W. F. J. Am. Chem. Soc. 1998, 120, 1138−1145]. roles intramolecular conformational dynamics folding kinetics in determining equilibrium properties have been investigated using novel NMR spin relaxation methods. To...
We report the design, synthesis, and characterization of a short peptide trapped in π-helix configuration. This high-energy conformation was nucleated by preorganized π-turn, which obtained replacing an N-terminal intramolecular main chain i + 5 hydrogen bond with carbon−carbon bond. Our studies highlight nucleation parameter as key factor contributing to relative instability allow us estimate fundamental helix−coil transition parameters for this conformation.
For human (HIV) and simian (SIV) immunodeficiency viruses, the gp41 envelope protein undergoes a receptor-activated conformational change from labile native structure to an energetically more stable fusogenic conformation, which then mediates viral-cell membrane fusion. The core of fusion-active is six-helix bundle in three antiparallel carboxyl-terminal helices are packed against amino-terminal trimeric coiled coil. Here we show that recombinant model SIV core, designated N36(L6)C34, forms...