A5101474342- Receptor Mechanisms and Signaling
- Mass Spectrometry Techniques and Applications
- Neuropeptides and Animal Physiology
- Drug Transport and Resistance Mechanisms
- Astro and Planetary Science
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- ZnO doping and properties
- Chemical Synthesis and Analysis
- Space Exploration and Technology
- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Pharmacological Receptor Mechanisms and Effects
- Nitric Oxide and Endothelin Effects
- Planetary Science and Exploration
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Tryptophan and brain disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- GaN-based semiconductor devices and materials
- Metabolism and Genetic Disorders
- Copper-based nanomaterials and applications
- Renin-Angiotensin System Studies
- Synthesis and Biological Evaluation
- Cannabis and Cannabinoid Research
- Gas Sensing Nanomaterials and Sensors
Peking University
2006-2025
Shandong University
2020-2025
Xi'an Jiaotong University
2022
University of Wisconsin–Milwaukee
2021
Institute of Biophysics
2019
Chinese Academy of Sciences
2019
National Ilan University
2002
Individual free fatty acids (FAs) play important roles in metabolic homeostasis, many through engagement with more than 40G protein-coupled receptors. Searching for receptors to sense beneficial omega-3 FAs of fish oil enabled the identification GPR120, which is involved a spectrum diseases. Here, we report six cryo-electron microscopy structures GPR120 complex FA hormones or TUG891 and Gi Giq trimers. Aromatic residues inside ligand pocket were responsible recognizing different double-bond...
Ceramides play a central role in human health and disease, yet their as systemic signaling molecules remain poorly understood. In this work, we identify FPR2 membrane receptor that specifically binds long-chain ceramides (C14-C20). brown beige adipocytes, C16:0 ceramide binding to inhibits thermogenesis via G i -cyclic AMP pathways, an effect is reversed the absence of FPR2. We present three cryo–electron microscopy structures complex with trimers bound C16:0, C18:0 C20:0 ceramides. The...
Abstract Characterization of the dynamic conformational changes in membrane protein signaling complexes by nuclear magnetic resonance (NMR) spectroscopy remains challenging. Here we report site-specific incorporation 4-trimethylsilyl phenylalanine (TMSiPhe) into proteins, through genetic code expansion. Crystallographic analysis revealed structural that reshaped TMSiPhe-specific amino-acyl tRNA synthetase active site to selectively accommodate trimethylsilyl (TMSi) group. The unique up-field...
Zn 1 − x Cd O films (0⩽x⩽0.179) were grown on Si (001) substrates at 750°C with a radio-frequency reactive magnetron sputtering method. Difference between the photoluminescence (PL) spectra taken room temperature (RT) and 12K is reported deduced to be result of PL emission from ZnCdO phases wurtzite zinc blende structures. It also found that RT intensity in inverse proportion carrier concentration films. incorporation results transform conductivity p type n decrease mobility.
The adaptive immune system plays a critical role in hyperhomocysteinemia (HHcy)-accelerated atherosclerosis. Recent studies suggest that HHcy aggravates atherosclerosis with elevated oxidative stress and reduced S-nitrosylation level of redox-sensitive protein residues the vasculature. However, whether how contributes to T-cell-driven remain unclear. In present study, we report T cells by inducing S-nitrosoglutathione reductase (GSNOR), key denitrosylase catalyzes (GSNO), which is main...
To accomplish concerted physiological reactions, nature has diversified functions of a single hormone at least two primary levels: 1) Different receptors recognize the same hormone, and 2) different cellular effectors couple to hormone–receptor pair [R.P. Xiao, Sci STKE 2001 , re15 (2001); L. Hein, J. D. Altman, B.K. Kobilka, Nature 402 181–184 (1999); Y. Daaka, M. Luttrell, R. Lefkowitz, 390 88–91 (1997)]. Not only these questions lie in heart actions receptor signaling but also dissecting...
Förster resonance energy transfer (FRET) is a well-established method for studying macromolecular interactions and conformational changes within proteins. Such normally uses fluorescent proteins or chemical-labeling methods which are often only accessible to surface-exposed residues risk-disturbing target protein structures. Here, we demonstrate that the genetic incorporation of synthetic amino acid, L-(7-hydroxycoumarin-4-yl) ethylglycine (Cou) natural endogenous fluorophore Tryptophan...
Urea's transmembrane transport through urea transporters (UT) is a fundamental physiological behavior for life activities. Here, we present 11 cryo-EM structures of four UT members in resting states, or inactive states bound with synthetic competitive, uncompetitive noncompetitive inhibitor. Our results indicate that the binding via conserved recognition motif (URM) and H-bond transfer along Q
In order to develop improved anxiolytic drugs, 8-substituted analogs of triazolam were synthesized in an effort discover compounds with selectivity for α2/α3 subunit-containing GABA A subtypes. Two this series, XLi-JY-DMH (6-(2-chlorophenyl)-8-ethynyl-1-methyl-4H-benzo [f][1,2,4]triazolo[4,3-a][1,4]diazepine) and SH-TRI-108 [(E)-8-ethynyl-1-methyl-6-(pyridin-2-yl)-4H-benzo [f][1,2,4]triazolo[4,3-a][1,4]diazepine], evaluated vitro vivo properties associated subtype-selective ligands....
Abstract G protein-coupled bile acid receptor (GPBAR) is a membrane that senses acids to regulate diverse functions through Gs activation. Here, we report the cryo-EM structures of GPBAR–Gs complexes stabilized by either high-affinity P395 or semisynthesized derivative INT-777 at 3-Å resolution. These revealed large oval-shaped ligand pocket with several sporadic polar groups accommodate amphipathic cholic core acids. A fingerprint key residues recognizing in orthosteric site, putative...
Abstract While one dimensional 1 H nuclear magnetic resonance (1D H-NMR) spectroscopy is of the most important and convenient method for measuring conformation change in biomacromolecules, characterization protein dynamics large membrane complexes by 1D H-NMR remains challenging, due to difficulty spectra assignment, low signal-to-noise ratio (S/N) need amount protein. Here we report site-specific incorporation 4-trimethylsilyl phenylalanine (TMSiPhe) into proteins, through genetic code...
Morphology and microstructure evolution of Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.3</sub> Ga xmlns:xlink="http://www.w3.org/1999/xlink">0.7</sub> N epilayers grown on GaN/sapphire templates with low-temperature (LT) AlN interlayers (ILs) by means metal organic chemical vapor deposition have been investigated transmission electron microscopy atomic force microscopy. It is found that the IL improves surface morphology, suppresses...