A5009578701- Gut microbiota and health
- Diet and metabolism studies
- Dietary Effects on Health
- Drug Transport and Resistance Mechanisms
- CRISPR and Genetic Engineering
- Geology and Paleoclimatology Research
- Paleontology and Stratigraphy of Fossils
- Clostridium difficile and Clostridium perfringens research
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Inflammatory Bowel Disease
- Carcinogens and Genotoxicity Assessment
- Electromagnetic Fields and Biological Effects
- Pediatric Hepatobiliary Diseases and Treatments
- Oral microbiology and periodontitis research
- Extracellular vesicles in disease
- RNA and protein synthesis mechanisms
- Effects and risks of endocrine disrupting chemicals
- Bacterial Genetics and Biotechnology
- Tryptophan and brain disorders
- RNA modifications and cancer
- Evolution and Genetic Dynamics
- RNA Research and Splicing
- Mycobacterium research and diagnosis
- Pregnancy and Medication Impact
Tianjin Medical University General Hospital
2024-2025
Capital Medical University
2025
BGI Group (China)
2015-2024
Chongqing Maternal and Child Health Hospital
2024
Wuhan University
2023-2024
Renmin Hospital of Wuhan University
2023-2024
Ministry of Education of the People's Republic of China
2012-2024
University of Toronto
2024
University Health Network
2024
Toronto General Hospital
2024
There is emerging evidence that the commensal microbiota has a role in pathogenesis of multiple sclerosis (MS), putative autoimmune disease CNS. Here, we compared gut microbial composition 34 monozygotic twin pairs discordant for MS. While there were no major differences overall profiles, found significant increase some taxa such as Akkermansia untreated MS twins. Furthermore, most notably, when transplanted to transgenic mouse model spontaneous brain autoimmunity, twin-derived induced...
Abstract Specific arrestin conformations are coupled to distinct downstream effectors, which underlie the functions of many G-protein-coupled receptors (GPCRs). Here, using unnatural amino acid incorporation and fluorine-19 nuclear magnetic resonance ( 19 F-NMR) spectroscopy, we demonstrate that receptor phospho-barcodes translated specific β-arrestin-1 direct selective signalling. With its phosphate-binding concave surface, ‘reads’ message in phospho-C-tails phospho-interaction patterns...
It is well known that the microbiota of high-fat (HF) diet-induced obese mice differs from lean mice, but to what extent, this difference reflects state or diet unclear. To dissociate changes in gut associated with high HF feeding those obesity, we took advantage different susceptibility C57BL/6JBomTac (BL6) and 129S6/SvEvTac (Sv129) obesity their responses inhibition cyclooxygenase (COX) activity, where COX activity BL6 prevents Sv129 accentuates obesity. Using HiSeq-based whole genome...
Objective Our primary objective is to phylogenetically characterize the supragingival plaque bacterial microbiome of children prior eruption second molars by pyrosequencing method for studying etiology early childhood caries. Methods Supragingival samples were collected from 10 caries and 9 caries-free children. Plaque DNA was extracted, used generate amplicons V1–V3 hypervariable region 16S rRNA gene, subjected 454-pyrosequencing. Results On average, over 22,000 sequences per sample...
Abstract The inflammatory intestinal disorder Crohn's disease (CD) has become a health challenge worldwide. gut microbiota closely interacts with the host immune system, but its functional impact in CD is unclear. Except for studies on small number of patients, analyses have used 16S rDNA amplicon sequencing. Here we employed metagenomic shotgun sequencing to provide detailed characterization compositional and features microbiota, comprising also unannotated bacteria, investigated modulation...
The oral cavity of each person is home to hundreds bacterial species. While taxa for diseases have been studied using culture-based characterization as well amplicon sequencing, metagenomic and genomic information remains scarce compared the fecal microbiome. Here, shotgun data 3346 samples together with 808 published samples, we obtain 56,213 metagenome-assembled genomes (MAGs), more than 64% 3589 species-level genome bins (SGBs) contain no publicly available genomes. resulting collection...
The proteolysis targeting chimera (PROTAC) strategy results in the down-regulation of unwanted protein(s) for disease treatment. In PROTAC process, a heterobifunctional degrader forms ternary complex with target protein interest (POI) and an E3 ligase, which ubiquitination proteasomal degradation POI. While formation is key attribute degraders, modification molecule to optimize can be labor-intensive tedious process. this study, we take advantage DNA-encoded library (DEL) technology...
Abstract Colorectal cancer (CRC) is a prevalent global health issue, with 5-fluorouracil (5-FU) being commonly used chemotherapeutic agent for its treatment. However, the efficacy of 5-FU often hindered by drug tolerance. Sodium butyrate (NaB), derivative intestinal flora, has demonstrated anti-cancer properties both in vitro and vivo through pro-apoptotic effects shown promise improving outcomes when conjunction traditional chemotherapy agents. This study seeks to evaluate impact potential...
Gossypium raimondii is a Verticillium wilt-resistant cotton species whose genome encodes numerous disease resistance genes that play important roles in the defence against pathogens. However, characteristics of gene analogues (RGAs) and dahliae response loci (VdRLs) have not been investigated on global scale. In this study, RGA were systematically analysed using bioinformatics-driven methods. Moreover, potential VdRLs involved to wilt identified by RNA-seq correlations with known QTLs.The G....
Abstract Macaca fascicularis, the cynomolgus macaque, is a widely used model in biomedical research and drug development as its genetics physiology are close to those of humans. Detailed information on macaque gut microbiota, functional interplay between microbiota host physiology, possible similarities humans other mammalians very limited. The aim this study was construct first microbial gene catalog compare human, pig, mouse catalogs. We performed metagenomic sequencing fecal samples from...
Traumatic brain injury (TBI) can induce systemic coagulopathy and inflammation, thereby increasing the risk of mortality disability. However, mechanism causing inflammation following TBI remains unclear. In prior research, we discovered that brain-derived extracellular vesicles (BDEVs), originating from injured brain, activate coagulation cascade inflammatory cells. this study, primarily investigated how BDEVs affect in peripheral circulation. The results cytokines function indicated lead to...
Female C57BL/6J mice were fed a regular low-fat diet or high-fat diets combined with either high low protein-to-sucrose ratios during their entire lifespan to examine the long-term effects on obesity development, gut microbiota, and survival. Intake of protein/sucrose ratio precipitated reduced survival relative diet. By contrast, intake attenuated lifelong weight gain adipose tissue expansion, was not significantly altered low-fat-fed mice. Our findings support notion that in response...
Major depressive disorder (MDD) seriously endangers adolescent mental and physical health. Extracellular vesicles (EVs) are mediators of cellular communication involved in many physiological brain processes. Although EV miRNAshave been implicated adults with major psychiatric disorders, investigation into their effects MDDremains scarce. In discovery set, we conducted a genome-wide miRNA sequencing serum EVs from 9 untreated adolescents MDD 8 matched healthy controls (HCs), identifying 32...
Long noncoding RNAs (lncRNAs) have been shown to play a critical role in cancer development and progression. LncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) is kidney cancer-associated onco-lncRNA involved the progression of renal cell carcinoma (RCC). However, pathological lncRNA MALAT-1 RCC proliferation metastasis remains poorly understood. This study was designed investigate biological mechanism metastasis. The experiments were performed human tissues, lines, nude...