Objective Our primary objective is to phylogenetically characterize the supragingival plaque bacterial microbiome of children prior eruption second molars by pyrosequencing method for studying etiology early childhood caries. Methods Supragingival samples were collected from 10 caries and 9 caries-free children. Plaque DNA was extracted, used generate amplicons V1–V3 hypervariable region 16S rRNA gene, subjected 454-pyrosequencing. Results On average, over 22,000 sequences per sample...

Abstract Background/Aim The extra‐alveolar period and storage medium are important for the survival of replanted teeth. aim this study was to evaluate factors affecting teeth in children. Material Methods Complete dental records avulsed including age, gender, time, type period, stage root development, crown fracture, gingival laceration, alveolar antibiotics, splint were obtained. Kaplan‐Meier, Cox regression chi‐square tests used analyse risk ( P < 0.05). Results included 196 with a mean...

We report the use of bacteriophage P1 Cre-lox system for generating conservative site-specific recombination between tobacco chromosomes. Two constructs, one containing a promoterless hygromycin-resistance gene preceded by lox site (lox-hpt) and other cauliflower mosaic virus 35S promoter linked to sequence cre coding region (35S-lox-cre), were introduced separately into plants. Crosses plants harboring either construct produced with two constructs situated on different Plants events...

As one of the most prevalent human infectious diseases, dental caries results from dysbiosis oral microbiota driven by multiple factors. However, little is known about distinct dynamics, and particularly change in species association pattern during disease transition. Here, we reported a twelve-month longitudinal study 144 3-year-old caries-free children. Oral examinations supragingival plaque collections were carried out at beginning every subsequent six months, for total three time points....

Abstract Aim To analyze the pulp prognosis of uncomplicated crown‐fractured teeth with or without concurrent luxation injury in adolescent. Material and methods Complete dental records traumatized permanent were obtained, including patient's name, gender age, position tooth, its stage root development, time elapsed between treatment, diagnosis, clinical procedures, follow‐up period. Pulp was evaluated over a period at least 6 months using Andreasen's classification. Kaplan‐Meier method Cox...

The major indications of a successful inflamed-pulp-capping procedure are the formation dense calcified dentin barrier and preservation healthy pulp tissue concomitant with elimination inflammation. Our aim is to evaluate effects an injectable silver-doped bioactive glass/chitosan hydrogel (Ag-BG/CS), as pulp-capping material, explore molecular mechanisms Ag-BG/CS in regards its anti-inflammatory properties. First, structure component material were analyzed by scanning electron microscopy....

Abstract Background/Aim Complicated crown fractures are relatively common in children where the maintenance of pulp is especially important young permanent teeth. The aim this study was to analyze prognosis teeth with complicated treated conservative treatment adolescents. Material and Methods Complete dental records were obtained. risk necrosis infection evaluated by Kaplan‐Meier method Cox regression ( P <.05). Risk factors included gender, age, time interval between injury treatment,...

Background Using salivary microbiota as an accurate proxy for monitoring supragingival remains controversial because their relationship unclear. The eruption of permanent teeth and the exfoliation primary in mixed dentition greatly alter microbial habitats, which may cause compositional shifts oral from childhood to adults. Objective This study's purpose was assess whether saliva represents a suitable sample healthy people, explore how replacement process deciduous with influences within...

Objectives The present study was designed to investigate the microbial profiles of teeth in different locations mixed-dentition-stage children, and compare microbiomes permanent deciduous same healthy oral cavity. Methods Supragingival plaque samples various locations—the first molars, canines incisors incisors—were collected from 20 children with 10–12 erupted. Plaque DNA extracted, V3–V4 hypervariable region bacterial 16S rRNA gene amplified subjected sequencing. Results On average, 18,051...

Children affected by severe early childhood caries (S-ECC) usually need comprehensive treatment due to the extensive of caries. How oral microbiome changes after therapy within short-term warrant further study.

Vital pulp therapy is critically important for the long-term preservation of teeth with pulpitis. However, anti-inflammatory effects pulp-capping materials used in clinics remain unsatisfactory. Pulpitis tightly connected oxidative stresses; therefore, scavenging excessive reactive oxygen species (ROS) a prospective treatment. Here thermosensitive and injectable hydrogel that forms situ was developed. By using poly(d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide)-based triblock copolymer...

The differences in microbiota between periodontitis and health have been extensively studied; however, knowledge about how the shifts from shallow to deep periodontal pockets remains limited despite its clinical importance disease progres-sion management. Patients diagnosed with stage III commonly pre-sent varied probing depths (PD) within same oral cavity, reflecting localized severity. This study aims analyze microbiome of subgingival plaques at various PDs patients. Subgingival were...

At Qinghai agriculture districts, Evergestis extimalis poses a significant threat to spring rapeseed cultivation through its larvae burring into the kernels and feeding seeds. To protect ecological environment of Qinghai–Tibet Plateau, it is essential research develop biological control technologies for pest management. In this study, we isolated purified new entomopathogenic fungus from carcasses E. larvae, which was identified as Mucor hiemalis based on morphological characteristics...

International Journal of Paediatric Dentistry 2011; 21: 422–431 Background. The genotypic diversity both Streptococcus mutans and sobrinus in children with different caries experience remains unclear. Aim. To investigate the S. severe early childhood (SECC) caries-free (CF) children. Methods. Stimulated saliva 87 SECC 91 CF aged 3–4 years was collected submitted to cultivation, MS colonies were enumerated. genomic fingerprint analysis carried out using AP-PCR. Results. One five genotypes...

Amelogenesis imperfecta is a group of inherited diseases affecting the quality and quantity dental enamel. To date, mutations in more than ten genes have been associated with non-syndromic amelogenesis (AI). Among these, ENAM LAMB3 are known to be parts etiology hypoplastic AI human cases. When both alleles defective, it could cause junctional epidermolysis bullosa (JEB), while only one mutant allele C-terminus LAMB3, result severe without skin fragility. We enrolled three Chinese families...

Oral microbiota that established in the early years of life may influence child's oral health long term. Until now, no consensus is reached about whether development more related with age increase or teeth eruption.To analyze both saliva and supragingival plaque during gradual eruption primary caries-free infants toddlers.Saliva samples were collected at five four dentition states, respectively, identified by bacterial 16S rRNA gene sequencing.During longitudinal observation, ecosystem...

Safe and effective biomaterials are in urgent clinical need for tissue regeneration bone repair. While numerous advances have been made on hydrogels promoting osteogenesis formation, co-stimulation of the angiogenic pathways this process remains to be exploited. Here, we developed a gelatin-based blue-light-curable hydrogel system, functionalized with an vascular endothelial growth factor (VEGF) mimetic peptide, KLTWQELYQLKYKGI (KLT), osteoanabolic parathyroid hormone (PTH) 1–34. We...