As one of the most prevalent human infectious diseases, dental caries results from dysbiosis oral microbiota driven by multiple factors. However, little is known about distinct dynamics, and particularly change in species association pattern during disease transition. Here, we reported a twelve-month longitudinal study 144 3-year-old caries-free children. Oral examinations supragingival plaque collections were carried out at beginning every subsequent six months, for total three time points....

Background Using salivary microbiota as an accurate proxy for monitoring supragingival remains controversial because their relationship unclear. The eruption of permanent teeth and the exfoliation primary in mixed dentition greatly alter microbial habitats, which may cause compositional shifts oral from childhood to adults. Objective This study's purpose was assess whether saliva represents a suitable sample healthy people, explore how replacement process deciduous with influences within...

Developing a simple and direct approach for interfacing sensor target analyte is of great interest fields such as medical diagnosis, threat detection, food quality control, environmental monitoring. Gloves provide unique interface sensing applications. Here, we show the first time development wearable carbon nanotube (CNT)-based amperometric biosensors painted onto gloves new platform, used here determination lactate. Three types were studied, configured as: two CNT electrodes; one...

Objectives The present study was designed to investigate the microbial profiles of teeth in different locations mixed-dentition-stage children, and compare microbiomes permanent deciduous same healthy oral cavity. Methods Supragingival plaque samples various locations—the first molars, canines incisors incisors—were collected from 20 children with 10–12 erupted. Plaque DNA extracted, V3–V4 hypervariable region bacterial 16S rRNA gene amplified subjected sequencing. Results On average, 18,051...

Oral microbiota that established in the early years of life may influence child's oral health long term. Until now, no consensus is reached about whether development more related with age increase or teeth eruption.To analyze both saliva and supragingival plaque during gradual eruption primary caries-free infants toddlers.Saliva samples were collected at five four dentition states, respectively, identified by bacterial 16S rRNA gene sequencing.During longitudinal observation, ecosystem...

The development of new bioelectronic platforms could provide exciting opportunities for clinical analysis, environmental monitoring, and defense. Wearable adhesive tapes adhere to skin be in close contact with sweat that consists many healthcare-related proteins, small molecules, ions, thus function as unique the sensor construction. Here, we show first time construction wearable tape-based smart biosensing systems, their determinations lactate glucose. is built by printing carbon graphite...

Tonsillar hypertrophy is a common disease in 3-to-6-year-old children, which may cause serve symptoms like airway obstruction. Microbiological factors play an important role the etiology of tonsillar hypertrophy. As starting point digestive and respiratory tracts, microbial composition oral cavity not only unique but also closely related to resident microbiota other body sites. Here we reported correlation study between tonsils children with Saliva, supragingival plaque, wiped samples from...

In this paper, we applied an analytical framework called "city cooperation intention → city relationship network regional economic resilience," while considering the concept of externality as a measure intention, to draw insights from perspectives relationships, biological evolution, and evolutionary economy. The evaluation system developed focuses on impact inherent culture inter-city network, using variables such knowledge spillover effect, technology symbiosis index, market structure. We...

Developing a simple and direct approach for sensitive, specific, rapid detection of metabolic compounds is great importance variety biological, medical, food applications. Tubes are highly portable accessible container shape which widely used scientific research in cell biology chemical synthesis, also use domestic health care Here, we show the first time development tube-based painted amperometric biosensor glucose lactate. The sensor was prepared by printing carbon graphite silver/silver...

The development of new sensing platforms for the rapid, real-time, and onsite detection metabolic compounds could provide significant opportunities medical science, healthcare applications. Microplates are standard tools that widely used in cell biology, tissue engineering, or diagnosis. Here, we demonstrate first time microplate-integrated amperometric biosensors rapid sensitive glucose lactate. sensor is constructed by printing carbon-graphite mediator paste as working electrode, Ag/AgCl...

The development of new bioelectronic platforms for direct interactions with oral fluid could open up significant opportunities healthcare monitoring. A tongue depressor is a widely used medical tool that inserted into the mouth, where it comes close contact saliva. Glucose typical salivary biomarker. Herein, we report-for first time-a depressor-based biosensor detection glucose in both phosphate buffer and real human Carbon nanotubes (CNTs) are attractive electronic materials, excellent...

Severe early childhood caries (S-ECC) is mainly caused by the interaction of microbiota and environmental factors. However, metabolic profiles S-ECC microbial communities community-level responses to carbohydrates amino acids are poorly understood.We collected supragingival plaques from 15 caries-free (CF) 14 children. Cultivation on Biolog AN microplates together with next-generation sequencing was used analyze sole carbon source utilization patterns sucrose, lactose phenylalanine.S-ECC had...

Abstract The development of a simple directly wearable approach for the rapid, specific and sensitive determination biomarkers is great importance to variety biomedical applications. Dental floss can provide unique device platform sensing oral biomarkers. We show here first time smart dental biosensing glucose. sensor was made by painting carbon graphite ink Ag/AgCl on floss. Via immobilization glucose oxidase, we detection with range 0.048 mM 19.5 response about 2 min. It expected that our...

Developing a new biosensing platform for the direct detection of analytes in beverages can offer significant, opportunities medical science, microbiology, nutrition studies, and food industry. Cork stoppers are widely used beverage bottles, stopper's inner sides be contact with beverages. Here, we show first time development an amperometric biosensor on cork two electrode configuration containing carbon nanotube as working Ag/AgCl reference electrode. The sensor displays sensitive hydrogen...

Increasing lines of evidence indicate that while microbial profile might vary, community-level metabolic potential is often more stably correlated with healthy and diseased states. Here, we investigated the diversity dental plaque microbiota from caries-free (CF) caries-affected (CA) children by measuring their sole carbon source utilization using a Biolog assay. The dietary habits 32 CF 31 CA were recorded questionnaire. Supragingival samples collected inoculated into AN Microplates to...

(1) Epidemiological studies have shown that deciduous molar caries are related to and more severe than permanent caries. This study aimed investigate whether subtypes in molars were associated with first explore taxonomic functional profiles of the microbiota involved different subtypes. (2) 42 mixed-dentition children recruited divided into DMC (carious but caries-free molars; n = 14), C 13), control (n 15) groups. Metagenomic sequencing was performed for supragingival plaque samples...

Objective: To record occlusal contact of primary dentition at maximal intercuspal position in children with individual normal occlusion. Methods: A total 57 were recruited from patients the Department Pediatric Dentistry, Peking University School and Hospital Stomatology. Inclusion criteria that subjects 3-5 years old no visually detectable caries or pupal periapical diseases, had complete dentition, occlusion, function craniofacial system, medically healthy, could cooperate sampling...

We report two kinds of method for preparing luminescent silicon films with quantum crystallites (QCs) structures: (1) Using laser annealing technique to crystallize ultrathin amorphous layers which were constructed in a-Si:H/a-SiN<SUB>x</SUB>:H multiquantum well (MQW) structures. (2) Applying the layer-by-layer deposition growth QCs by varying hydrogen plasma exposure time. The novel structures these types characterized X-ray diffraction and Raman scattering spectroscopy. room temperature...