Significance The Candidate Phyla Radiation (CPR) is a large monophyletic lineage with poorly understood biology. Saccharibacteria are ultrasmall parasitic CPR bacteria highly reduced genomes that have made the transition from an environmental origin to mammals. We tested function and impact of arginine deiminase system (ADS), catabolism pathway likely acquired by mammal-associated during their environment-to-mammal niche transition. showed ADS not only helped facilitate Saccharibacterial...

Recent characterization of the obligate episymbiont Saccharibacteria (TM7) belonging to candidate phyla radiation (CPR) has expanded extent microbial diversity. However, episymbiotic lifestyle TM7 is still underexploited due deficiency cultivated representatives. Here, we describe gene-targeted cultivation guided by repurposing epicPCR (emulsion, paired isolation, and concatenation PCR) capture in situ TM7‒host associations. Using this method, obtained a novel isolate TM7i its host...

Abstract Saccharibacteria (formerly TM7) are a group of widespread and genetically diverse ultrasmall bacteria with highly reduced genomes that belong to Candidate Phyla Radiation, large monophyletic lineage poorly understood biology. Nanosynbacter lyticus type strain TM7x is the first member isolated from human oral microbiome. With restrained metabolic capacities, lives on surface of, forms an obligate episymbiotic relationship its bacterial host, Schaalia odontolytica XH001. The symbiosis...

As one of the most prevalent human infectious diseases, dental caries results from dysbiosis oral microbiota driven by multiple factors. However, little is known about distinct dynamics, and particularly change in species association pattern during disease transition. Here, we reported a twelve-month longitudinal study 144 3-year-old caries-free children. Oral examinations supragingival plaque collections were carried out at beginning every subsequent six months, for total three time points....

Background Using salivary microbiota as an accurate proxy for monitoring supragingival remains controversial because their relationship unclear. The eruption of permanent teeth and the exfoliation primary in mixed dentition greatly alter microbial habitats, which may cause compositional shifts oral from childhood to adults. Objective This study's purpose was assess whether saliva represents a suitable sample healthy people, explore how replacement process deciduous with influences within...

Riverine blooms have become a challenging global environmental problem owing to strong disturbances from intensified human activities and the construction or operation of hydraulic projects. Previous studies mainly paid attention algal in lakes reservoirs, while less focused on prediction prevention large rivers. As one highly regulated rivers China, downstream Han River frequently occurred consecutive recent decades, was selected as case study. Firstly, during 1992–2021 were investigated...

Children affected by severe early childhood caries (S-ECC) usually need comprehensive treatment due to the extensive of caries. How oral microbiome changes after therapy within short-term warrant further study.

Oral microbiota that established in the early years of life may influence child's oral health long term. Until now, no consensus is reached about whether development more related with age increase or teeth eruption.To analyze both saliva and supragingival plaque during gradual eruption primary caries-free infants toddlers.Saliva samples were collected at five four dentition states, respectively, identified by bacterial 16S rRNA gene sequencing.During longitudinal observation, ecosystem...

Measure synchronization (MS) in a two-species bosonic Josephson junction (BJJ) is studied based on semi-classical theory. Six different scenarios for MS, including two the oscillation regime (0 phase mode) and four self-trapping ($\pi$ mode), have been clearly shown. Systematic investigations of common features behind these performed. We show that average energies species merge at MS transition point. The scaling power law near has verified, critical exponent 1/2 all MS. also illustrate...

Tonsillar hypertrophy is a common disease in 3-to-6-year-old children, which may cause serve symptoms like airway obstruction. Microbiological factors play an important role the etiology of tonsillar hypertrophy. As starting point digestive and respiratory tracts, microbial composition oral cavity not only unique but also closely related to resident microbiota other body sites. Here we reported correlation study between tonsils children with Saliva, supragingival plaque, wiped samples from...

Severe early childhood caries (S-ECC) is mainly caused by the interaction of microbiota and environmental factors. However, metabolic profiles S-ECC microbial communities community-level responses to carbohydrates amino acids are poorly understood.We collected supragingival plaques from 15 caries-free (CF) 14 children. Cultivation on Biolog AN microplates together with next-generation sequencing was used analyze sole carbon source utilization patterns sucrose, lactose phenylalanine.S-ECC had...

Abstract Data transformation of the reference soil organic matter (SOM) decomposition rates ( k ref ), often derived as turnover times or in alternative formats, is commonly used to develop ecological models for projecting persistence SOM. However, effects reciprocal logarithmic on model performance and edaphic‐climatic patterns remain uncertain. Here, we convert published values into formats establish machine learning between transformed predictors. We show that trained with exhibit a...

Abstract Saccharibacteria (formerly TM7) Nanosynbacter lyticus type strain TM7x exhibits a remarkably compact genome and an extraordinarily small cell size. This obligate epibiotic parasite forms symbiotic relationship with its bacterial host, Schaalia odontolytica , XH001 Actinomyces odontolyticus XH001). Due to limited size, possesses restrained metabolic capacities, predominantly living on the surface of host sustain this lifestyle. To comprehend intriguing, yet understudied interspecies...

The synergistic effect of microbial consortium S3 (including S3-1, S3-2, S3-3 and S3-4), which has the ability degrading Polycyclic Aromatic Hydrocarbons was studied by solid phenanthrene as sole source carbon energy. research showed that only S3-1 had characteristics phenanthrene-degrading other three strains could use metabolites in different degree. It found distinct producing biosurfactants. a faster degradation rate compared with it conjectured there were two reasons: (1) might reduce...

Increasing lines of evidence indicate that while microbial profile might vary, community-level metabolic potential is often more stably correlated with healthy and diseased states. Here, we investigated the diversity dental plaque microbiota from caries-free (CF) caries-affected (CA) children by measuring their sole carbon source utilization using a Biolog assay. The dietary habits 32 CF 31 CA were recorded questionnaire. Supragingival samples collected inoculated into AN Microplates to...

In order to reduce production cost of Pseudomonas aeruginosa T1, which has the ability producing biosurfactant when using crude oil as sole carbon source, Three experimental methodologies (Plackett-Burman design (PBD), steepest ascent (SAD), Central composite rotatable (CCRD)) were applied optimize medium ingredients strain. verification experiment, surface tension (ST) strain T1 fermentation decreased from 29.93mN/m 27.08mN/m after optimization.

Liquid crystal was formed in a ternary system of tetraethylene glycol lauryl ether, water and 1-ethyl-3-methylimidazolium hexafluorophosphate. Its structure identified by polarized optical microscopy small-angle X-ray scattering techniques. Compared to that the binary system, lamellar phase retained with addition small amount imidazolium salt. Molecules hexafluorophosphate were considered be solubilized polar domain ordered assemblies. Results on their influence enlighten use salts modulate...

(1) Epidemiological studies have shown that deciduous molar caries are related to and more severe than permanent caries. This study aimed investigate whether subtypes in molars were associated with first explore taxonomic functional profiles of the microbiota involved different subtypes. (2) 42 mixed-dentition children recruited divided into DMC (carious but caries-free molars; n = 14), C 13), control (n 15) groups. Metagenomic sequencing was performed for supragingival plaque samples...

Realgar transforming solution is an arsenic formulation which has shown anticancer effects with low toxicity both in vivo and vitro. In this study, Caenorhabditis elegans was used to evaluate its reproductive possible mechanisms. decreased the brood size induced proliferation arrest apoptosis significantly only at elemental concentration of 37.5 mg/L, while trioxide reduced sizes wild type N2 let-60 ras(gf) mutant worms dependent manner. Mitogen-activated protein kinase p53 pathways may be...