A5046448590- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Mycorrhizal Fungi and Plant Interactions
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Polysaccharides and Plant Cell Walls
- Pesticide Residue Analysis and Safety
- Plant and Fungal Interactions Research
- Legume Nitrogen Fixing Symbiosis
- Research in Cotton Cultivation
- Fermentation and Sensory Analysis
- Genomics and Phylogenetic Studies
- Horticultural and Viticultural Research
- Microbial Community Ecology and Physiology
- Plant Parasitism and Resistance
- Plant pathogens and resistance mechanisms
- Plant Physiology and Cultivation Studies
- Plant Gene Expression Analysis
- Analytical Chemistry and Chromatography
- Analytical chemistry methods development
- Phytochemicals and Antioxidant Activities
- Insect-Plant Interactions and Control
Institute of Plant Protection
2020-2025
Chinese Academy of Agricultural Sciences
2016-2025
State Key Laboratory of Biology of Plant Diseases and Insect Pests
2024-2025
Xinjiang Academy of Agricultural Sciences
2024
Shenzhen University
2023
Sun Yat-sen University
2019-2023
Hospital of Stomatology, Sun Yat-sen University
2019-2023
Stomatology Hospital
2019-2023
Waseda University
2022
Ten Chen Hospital
2021
Glycoside hydrolase 12 (GH12) proteins act as virulence factors and pathogen-associated molecular patterns (PAMPs) in oomycetes. However, the pathogenic mechanisms of fungal GH12 have not been characterized. In this study, we demonstrated that two six produced by fungus Verticillium dahliae Vd991, VdEG1 VdEG3 acted PAMPs to trigger cell death PAMP-triggered immunity (PTI) independent their enzymatic activity Nicotiana benthamiana. A 63-amino-acid peptide was sufficient for death-inducing...
Summary Verticillium wilt ( VW ), caused by infection dahliae , is considered one of the most yield‐limiting diseases in cotton. To examine genetic architecture cotton resistance, we performed a genome‐wide association study GWAS ) using panel 299 accessions and 85 630 single nucleotide polymorphisms SNP s) detected specific‐locus amplified fragment sequencing SLAF ‐seq) approach. Trait– analysis total 17 significant s at P < 1.17 × 10 –5 = 1/85 630, –log 4.93); peaks associated with...
Verticillium dahliae isolates are most virulent on the host from which they were originally isolated. Mechanisms underlying these dominant adaptations currently unknown. We sequenced genome of V. Vd991, is highly its original host, cotton, and performed comparisons with reference genomes JR2 (from tomato) VdLs.17 lettuce). Pathogenicity-related factor prediction, orthology multigene family classification, transcriptome analyses, phylogenetic pathogenicity experiments performed. The Vd991...
Abstract Herbivore‐induced terpenes have been reported to function as ecological signals in plant–insect interactions. Here, we showed that insect‐induced cotton volatile blends contained 16 terpenoid compounds with a relatively high level of linalool. The diversity terpene production is derived from large synthase (TPS) gene family. TPS family Gossypium hirsutum and raimondii consist 46 41 members, respectively. Twelve genes ( GhTPS4–15 ) could be isolated, protein expression Escherichia...
Verticillium wilt, caused by the dahliae phytopathogen, is a devastating disease affecting many economically important crops. Previous studies have shown that exoproteome of V. plays significant role in this pathogenic process, but components and mechanisms underlie remain unclear. In study, was induced cotton-containing C'zapek-Dox (CCD) medium quantified using high-throughput isobaric tag technique for relative absolute quantification (iTRAQ). Results showed abundance 271 secreted proteins...
Gossypium raimondii is a Verticillium wilt-resistant cotton species whose genome encodes numerous disease resistance genes that play important roles in the defence against pathogens. However, characteristics of gene analogues (RGAs) and dahliae response loci (VdRLs) have not been investigated on global scale. In this study, RGA were systematically analysed using bioinformatics-driven methods. Moreover, potential VdRLs involved to wilt identified by RNA-seq correlations with known QTLs.The G....
Secreted small cysteine-rich proteins (SCPs) play a critical role in modulating host immunity plant-pathogen interactions. Bioinformatic analyses showed that the fungal pathogen Verticillium dahliae encodes more than 100 VdSCPs, but their roles host-pathogen interactions have not been fully characterized. Transient expression of 123 VdSCP-encoding genes Nicotiana benthamiana identified three candidate involved The these proteins, VdSCP27, VdSCP113, and VdSCP126, N. resulted cell death...
Verticillium dahliae is a broad host-range pathogen that causes vascular wilts in plants. Interactions between three hosts and specific V. genotypes result severe defoliation. The underlying mechanisms of defoliation are unresolved. Genome resequencing, gene deletion complementation, expression analysis, sequence divergence, defoliating phenotype identification, virulence quantification secondary metabolites were performed. Population genomics previously revealed G-LSR2 was horizontally...
Improving genetic resistance is a preferred method to manage Verticillium wilt of cotton and other hosts. Identifying host difficult because the dearth genes against this pathogen. Previously, novel candidate gene involved in was identified by genome-wide association study using panel Gossypium hirsutum accessions. In study, we cloned from that encodes protein sharing homology with TIR-NBS-LRR receptor-like defence DSC1 Arabidopsis thaliana (hereafter named GhDSC1). GhDSC1 expressed at...
Abstract Background Verticillium dahliae is a fungal pathogen that causes vascular wilt on many economically important crops. Common extracellular membrane (CFEM) domain proteins including secreted types have been implicated in virulence, but their roles this are still unknown. Results Nine small cysteine-rich (VdSCPs) with CFEM domains were identified by bioinformatic analyses and differential suppression of host immune responses evaluated. Two these proteins, VdSCP76 VdSCP77, localized to...
Searching the economical, eco-friendly and efficient biological control measures is key to protecting crops from pathogenic fungi. The species of Burkholderia genus are widespread in natural environment, which nonpathogenic members have been reported great potential for agents biofertilizers agricultural application. gladioli strains, however, need more study application fungi, plant growth promotion, induced systemic resistance (ISR).
Abstract In response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. However, little is known about the molecular genomic basis defense that herbivory trigger in cotton how mechanisms are orchestrated context other biological processes. Here we monitored transcriptome changes characteristics bollworm (CBW; Helicoverpa armigera ) larvae infestation. Analysis samples revealed 1,969 transcripts were differentially expressed (log...
Cutinases have been implicated as important enzymes during the process of fungal infection aerial plant organs. The function cutinases in disease cycle pathogens that invade plants through roots has less studied. Here, functional analysis 13 cutinase (carbohydrate esterase family 5 domain-containing) genes (VdCUTs) highly virulent vascular wilt pathogen Verticillium dahliae Vd991 was performed. Significant sequence divergence members observed genome V. Vd991. Functional analyses demonstrated...
Verticillium dahliae is a phytopathogenic fungus that causes vascular wilt disease in wide variety of crop plants, thereby causing extensive economic loss. In present study, one V. T-DNA mutant M01C06 showed the pathogenicity loss on cotton, and expression flanking gene encoding cytochrome P450 monooxygenase (P450, VdCYP1) was strongly repressed. P450s fungi could affect fungal by involving synthesis secondary metabolites. However, there no report about pathogenic function dahliae. VdCYP1...
Summary Fungal transcription factors (TFs) implicated in the regulation of virulence gene expression have been identified a number plant pathogens. In Verticillium dahliae , despite its agricultural importance, few regulators characterized. this study, T‐DNA insertion mutant with significantly reduced towards cotton was identified. The traced to VdFTF1 encoding TF containing Fungal_trans domain. Transient onion epidermal cells indicated that is localized nucleus. VdFTF1‐ deletion strains...
Phytopathogen xylanases play critical roles in pathogenesis, likely due to their ability degrade plant structural barriers and manipulate host immunity. As an invader of xylem vessels, the fungus Verticillium dahliae is thought deploy complex cell wall degrading enzymes. Comparative genomics analyses revealed that V. genome encodes a family six xylanases, each possessing glycosyl hydrolase 11 domain, but functions these enzymes are undetermined. Characterizing gene deletion mutants only...
Abstract The accumulation of reactive oxygen species (ROS) is a widespread defence mechanism in higher plants against pathogen attack and sometimes the cause cell death that facilitates by necrotrophic pathogens. Plant pathogens use superoxide dismutase (SOD) to scavenge ROS derived from their own metabolism or generated host defence. significance roles SODs vascular plant Verticillium dahliae are unclear. Our previous study showed significant upregulation Cu/Zn‐SOD1 (VdSOD1) cotton tissues...
Abstract Background During the disease cycle, plant pathogenic fungi exhibit a morphological transition between hyphal growth (the phase of active infection) and production long-term survival structures that remain dormant during “overwintering.” Verticillium dahliae is major pathogen produces heavily melanized microsclerotia (MS) survive in soil for 14 or more years. These MS are multicellular produced necrotrophic cycle. Polyketide synthases (PKSs) responsible catalyzing many secondary...
Abstract The arms race between fungal pathogens and plant hosts involves recognition of effectors to induce host immunity. Although various have been identified, the effector functions ribonucleases are largely unknown. Herein, we identified a ribonuclease secreted by Verticillium dahliae (VdRTX1) that translocates into nucleus modulate activity VdRTX1 causes hypersensitive response (HR)‐related cell death in Nicotiana benthamiana cotton. possesses signal peptide but is unlikely be an...
Abstract Background Verticillium wilt, caused by the fungus dahliae , is a soil-borne vascular fungal disease, which has great losses to cotton yield and quality worldwide. The strain KRS010 was isolated from seed of wilt-resistant Gossypium hirsutum cultivar “Zhongzhimian No. 2.” Results broad-spectrum antifungal activity various pathogenic fungi as Botrytis cinerea Fusarium spp., Colletotrichum Magnaporthe oryzae inhibition rate V. mycelial growth 73.97% 84.39% respectively through...