A5008037802- Bone Metabolism and Diseases
- Cancer-related molecular mechanisms research
- Protein Interaction Studies and Fluorescence Analysis
- Bone health and treatments
- MicroRNA in disease regulation
- Wnt/β-catenin signaling in development and cancer
- Otolaryngology and Infectious Diseases
- Cancer-related gene regulation
- Infectious Diseases and Tuberculosis
- TGF-β signaling in diseases
- Drug Transport and Resistance Mechanisms
- Plant-Microbe Interactions and Immunity
- Salivary Gland Disorders and Functions
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Osteoarthritis Treatment and Mechanisms
- RNA Research and Splicing
- NF-κB Signaling Pathways
- interferon and immune responses
- Temporomandibular Joint Disorders
- Monoclonal and Polyclonal Antibodies Research
- Streptococcal Infections and Treatments
- Bacteriophages and microbial interactions
- Metal complexes synthesis and properties
- Orthopedic Infections and Treatments
Tianjin Medical University
2016-2025
Shanghai Ninth People's Hospital
2012-2025
Shanghai Jiao Tong University
2012-2025
National Clinical Research Center for Digestive Diseases
2018-2025
Institute of Endocrinology
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2019-2024
Shanghai Stomatological Hospital
2019-2024
Tianjin University of Technology and Education
2023
Hainan Normal University
2023
Yuncheng University
2022-2023
While transforming growth factor β (TGFβ) signaling plays a critical role in chondrocyte metabolism, the TGFβ pathways and target genes involved cartilage homeostasis development of osteoarthritis (OA) remain unclear. Using an vitro cell culture method vivo mouse genetic approach, we undertook this study to investigate chondrocytes determine whether Mmp13 Adamts5 are downstream signaling.TGFβ receptor type II (TGFβRII)-conditional knockout (KO) (TGFβRII(Col2ER)) mice were generated by...
Verticillium dahliae isolates are most virulent on the host from which they were originally isolated. Mechanisms underlying these dominant adaptations currently unknown. We sequenced genome of V. Vd991, is highly its original host, cotton, and performed comparisons with reference genomes JR2 (from tomato) VdLs.17 lettuce). Pathogenicity-related factor prediction, orthology multigene family classification, transcriptome analyses, phylogenetic pathogenicity experiments performed. The Vd991...
Abstract Epiregulin plays a role in range of biological activities including malignancies. This study aims to investigate the potential contribution epiregulin bone cell differentiation and homeostasis. The data showed that expression was upregulated during osteogenesis but downregulated adipogenesis. Functionally, promoted osteoblast while inhibiting adipocyte from mesenchymal progenitor cells. Epidermal growth factor receptor (EGFR), one two known receptors for epiregulin, exerted opposing...
Abstract Objective The incidence of low back pain is extremely high and often linked to intervertebral disc (IVD) degeneration. mechanism this disease currently unknown. This study was undertaken investigate the role β‐catenin signaling in IVD tissue function. Methods protein levels were measured by immunohistochemical analysis samples obtained from patients with degeneration normal subjects. To generate conditional activation (cAct) mice, Col2a1‐CreER T2 –transgenic mice bred...
In chondrocytes, PTHrP maintains them in a proliferative state and prevents premature hypertrophy. The mechanism by which does this is not fully understood. Both Runx2 Runx3 are required for chondrocyte maturation. We recently demonstrated that cyclin D1 induces protein phosphorylation degradation. the present studies, we tested hypothesis regulates both stability through D1. analyzed effects of on function using COS cells, osteoprogenitor C3H10T1/2 cells chondrogenic RCJ3.1C5.18 cells....
TGF-β signaling plays a critical role in cartilage and spine tissue development at embryonic stage but its postnatal intervertebral disc (IVD) growth maintenance remain poorly understood. In the present studies, we have deleted Tgfbr2 gene inner annulus fibrosus cells of surrounding plate chondrocytes using Col2a1-CreERT2 transgenic mice. We found that is required for normal endplate stage. The expression Mmp13 significantly up-regulated primary conditional knockout Deletion under null...
Abstract Runx2 may play an important role in development of osteoarthritis (OA). However, the specific articular chondrocyte function and OA adult mice has not been fully defined. In this study, we performed destabilization medial meniscus (DMM) surgery at 12-week-old to induce Agc1CreER mice, which was specifically deleted Aggrecan -expressing chondrocytes by administering tamoxifen 8-weeks age. Knee joint samples were collected 8- 12-weeks post-surgery analyzed through histology,...
Abstract Several miRNAs have recently been identified to regulate adipocyte or osteoblast differentiation both. In this study, miR-223 was found be involved in the reciprocal regulation of and differentiation. induced primary cultured mouse marrow stromal cell, mesenchymal line C3H10T1/2 ST2 after adipogenic treatment. Conversely, it reduced preosteoblast MC3T3-E1 osteogenic Supplementing levels using synthetic mimics significantly suppressed growth cells progenitor fully differentiate into...
Secreted small cysteine-rich proteins (SCPs) play a critical role in modulating host immunity plant-pathogen interactions. Bioinformatic analyses showed that the fungal pathogen Verticillium dahliae encodes more than 100 VdSCPs, but their roles host-pathogen interactions have not been fully characterized. Transient expression of 123 VdSCP-encoding genes Nicotiana benthamiana identified three candidate involved The these proteins, VdSCP27, VdSCP113, and VdSCP126, N. resulted cell death...
Verticillium dahliae is a broad host-range pathogen that causes vascular wilts in plants. Interactions between three hosts and specific V. genotypes result severe defoliation. The underlying mechanisms of defoliation are unresolved. Genome resequencing, gene deletion complementation, expression analysis, sequence divergence, defoliating phenotype identification, virulence quantification secondary metabolites were performed. Population genomics previously revealed G-LSR2 was horizontally...
Improving genetic resistance is a preferred method to manage Verticillium wilt of cotton and other hosts. Identifying host difficult because the dearth genes against this pathogen. Previously, novel candidate gene involved in was identified by genome-wide association study using panel Gossypium hirsutum accessions. In study, we cloned from that encodes protein sharing homology with TIR-NBS-LRR receptor-like defence DSC1 Arabidopsis thaliana (hereafter named GhDSC1). GhDSC1 expressed at...
Acinar epithelial cell atrophy in secretory glands is a hallmark of primary Sjögren's syndrome (pSS), the cause which far from elucidated.We examined role acinar by focusing on metabolism glandular cells and mitochondria pSS environment.After confirming presence high-lactate environment labial human patients, we used A253 line NOD/Ltj mice as models to investigate metabolic changes salivary gland vitro vivo.We found that produced high levels IL-6, IL-8, IFN-α, IFN-β TNF-α exhibited...
Abstract Histone methylation plays a crucial role in various cellular processes. We previously reported the vitro function of histone lysine demethylase 7 A (KDM7A) osteoblast and adipocyte differentiation. The current study was undertaken to investigate physiological KDM7A bone homeostasis elucidate underlying mechanisms. conditional strategy employed delete Kdm7a gene specifically osterix-expressing osteoprogenitor cells mice. resulting mutant mice exhibited significant increase cancellous...
Cutinases have been implicated as important enzymes during the process of fungal infection aerial plant organs. The function cutinases in disease cycle pathogens that invade plants through roots has less studied. Here, functional analysis 13 cutinase (carbohydrate esterase family 5 domain-containing) genes (VdCUTs) highly virulent vascular wilt pathogen Verticillium dahliae Vd991 was performed. Significant sequence divergence members observed genome V. Vd991. Functional analyses demonstrated...
Abstract Recent emerging studies of miRNAs in adipocyte commitment provide new insights to understand the molecular basis adipogenesis. The current study indicated that miR-140-5p was altered primary cultured marrow stromal cells and established progenitor lines after adipogenic and/or osteogenic treatment. increased adipose tissue db/db obese mice vs. lean mice. Supplementing activity induced cell ST2 preadipocyte 3T3-L1 differentiate into mature adipocytes. Conversely, inhibition...