A5038554784- Clostridium difficile and Clostridium perfringens research
- Viral gastroenteritis research and epidemiology
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Inflammasome and immune disorders
- Pancreatitis Pathology and Treatment
- Cancer therapeutics and mechanisms
- Bacterial biofilms and quorum sensing
- Antibiotics Pharmacokinetics and Efficacy
- IL-33, ST2, and ILC Pathways
- Neuroendocrine Tumor Research Advances
- Gout, Hyperuricemia, Uric Acid
- Biological Research and Disease Studies
- Bacterial Identification and Susceptibility Testing
- Cholesterol and Lipid Metabolism
- interferon and immune responses
- Genetics, Bioinformatics, and Biomedical Research
- Case Reports on Hematomas
- Glycosylation and Glycoproteins Research
- Image Processing Techniques and Applications
- Pharmacological Effects of Natural Compounds
- Nutrition, Genetics, and Disease
Infectious Disease Research Institute
2022-2025
Texas A&M Health Science Center
2016-2023
Lokmanya Tilak Municipal General Hospital and Lokmanya Tilak Municipal Medical College
2021
College of Medicine & JNM Hospital
2021
Severe outbreaks and deaths have been linked to the emergence global spread of fluoroquinolone-resistant Clostridioides difficile over past two decades. At same time, metronidazole, a nitro-containing antibiotic, has shown decreasing clinical efficacy in treating C. infection (CDI). Most metronidazole-resistant exhibit an unusual resistance phenotype that can only be detected susceptibility tests using molecularly intact heme. Here, we describe mechanism underlying this trait. We find most...
To describe, for the first time (to best of our knowledge), genetic mechanisms vancomycin resistance in clinical isolates Clostridioides difficile ribotype 027.
Tuberculosis (TB), caused by Mycobacterium tuberculosis , remains a significant global health threat, affecting an estimated 10.6 million people in 2022. The emergence of multidrug resistant and extensively drug strains necessitates the development novel effective drugs. Accelerating determination mechanisms action (MOAs) for these drugs is crucial advancing TB treatment. This study introduces MycoBCP, unique adaptation bacterial cytological profiling (BCP) tailored to M. utilizing...
Chromosomal resistance to metronidazole has emerged in clinical Clostridioides difficile isolates, but the genetic mechanisms remain unclear. This is further hindered by inability generate spontaneous metronidazole-resistant mutants lab interpret variations isolates. We therefore constructed a mismatch repair mutator nontoxigenic ATCC 700057 survey mutational landscape for de novo mechanisms. In separate experimental evolutions, adopted deterministic path resistance, with truncation of...
Clinical studies have demonstrated inferior cure rates when metronidazole (MTZ) is used to treat
Limitations in efficacy and/or tolerance of currently available urate-lowering therapies (ULTs), such as oral xanthine oxidase inhibitors, uricosurics, and intravenous uricase agents contribute to the development refractory gout. Renal excretion is major route uric acid elimination, but intestinal tract plays an increasingly recognized role urate homeostasis, particularly chronic kidney disease (CKD) which renal elimination impaired. We targeted degradation vivo with ALLN-346, orally...
Metronidazole resistance in clinical Clostridioides difficile is often described as unstable, since resistant strains reportedly appear susceptible following freezer storage or brief passage. This has presented a conundrum for adopting susceptibility testing to accurately evaluate the connection between metronidazole and decreased efficacy of patients with C. infections (CDIs). We discovered that supplementation microbiological media metalloporphyrin heme crucial detection...
ABSTRACT Ferrous iron is the dominant form of in oxygen-limited large intestine, site for Clostridioides difficile infection (CDI). We investigated extent to which C. requires ferrous transporter 1 (FeoB1), a main permease acquisition, using vitro and vivo approaches. Construction feoB1 deletion mutant R20291 (i.e., R20291Δ ) decreased intracellular content by ~25%. This was accompanied reduced synthesis TcdA TcdB toxins downregulation tcdA tcdB genes, reflected ~1000-fold reduction...
Abstract Severe outbreaks and deaths have been linked to the emergence global spread of fluoroquinolone-resistant Clostridioides difficile over past two decades. At same time, metronidazole, a nitro-containing antibiotic, has shown decreasing clinical efficacy in treating C. infection (CDI). Most metronidazole-resistant exhibit an unusual resistance phenotype that can only be detected susceptibility tests utilizing molecularly intact heme. Here we describe mechanism underlying this trait,...
Abstract Chromosomal resistance to metronidazole has emerged in clinical Clostridioides difficile , but the genetic mechanisms remain unclear. This is further hindered by inability generate spontaneous metronidazole-resistant mutants lab aid studies. We therefore constructed a mismatch repair mutator, non-toxigenic ATCC 700057, unbiasedly survey mutational landscape for de novo mechanisms. In separate experimental evolutions, mutator adopted deterministic path resistance, with truncation of...
The NLRP3 inflammasome is an intracellular protein complex that causes inflammation via the release of IL-1β and pyroptosis. activation associated with many age-related inflammatory diseases, inhibition a promising therapeutic strategy. We previously performed DNA encoded library screen to identify novel binding molecules. Herein we describe characterization BAL-0028 as potent specific inhibitor signaling. Notably, poor mouse but inhibits human primate nanomolar potency. Using cellular...
Background: In the view of need for early identification cases Acute Pancreatitis [AP] who may end up dying in spite rigorous treatment measures, many scores have been proposed claiming to be superior doing so.This study compares 5 newer which are possible assessed first few hours admission with mortality as outcome.Methods: It's a retrospective 200 consecutive episodes AP tertiary care centre India.Scores were compared at their optimal cut off values by sensitivity, specificity, Positive...
Indolcarboxamides are a promising series of anti-tubercular agents which target
Indolcarboxamides are a promising series of anti-tubercular agents, which target
Abstract Background Current guidelines suggest limiting metronidazole (MTZ) use due to increased treatment failures in patients with Clostridioides difficile infections (CDI). We hypothesized that an increase the minimum inhibitory concentration (MIC) of MTZ C. may contribute these poor response rates. The objective this study was examine clinical rates CDI based on MIC and stratified by receipt treatment. Methods difficile-positive stool samples collected from 2017 2018 as part routine care...