A5115602793- CRISPR and Genetic Engineering
- Genetic Mapping and Diversity in Plants and Animals
- Extracellular vesicles in disease
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- MicroRNA in disease regulation
- Plant Gene Expression Analysis
- Genetic Syndromes and Imprinting
- RNA and protein synthesis mechanisms
- Cell Adhesion Molecules Research
- Protein Kinase Regulation and GTPase Signaling
- Epigenetics and DNA Methylation
- Endoplasmic Reticulum Stress and Disease
- GABA and Rice Research
- Hedgehog Signaling Pathway Studies
- Connexins and lens biology
- Rice Cultivation and Yield Improvement
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Nuclear Structure and Function
- Synthesis and Biological Activity
- Hippo pathway signaling and YAP/TAZ
- Kruppel-like factors research
- Angiogenesis and VEGF in Cancer
- Electron Spin Resonance Studies
Chinese Academy of Agricultural Sciences
2017-2024
Institute of Crop Sciences
2017-2024
Cold Spring Harbor Laboratory
2022-2024
Case Western Reserve University
2024
First Affiliated Hospital of Jiangxi Medical College
2024
Nanchang University
2023-2024
Ministry of Agriculture and Rural Affairs
2022-2024
Shanghai Veterinary Research Institute
2020-2024
Xinjiang Agricultural University
2023
Institute of Vegetables and Flowers
2023
SorghumBase provides a community portal that integrates genetic, genomic, and breeding resources for sorghum germplasm improvement. Public research development in agriculture rely on proper data resource sharing within stakeholder communities. For plant breeders, agronomists, molecular biologists, geneticists, bioinformaticians, centralizing desirable into user-friendly hub crop systems is essential successful collaborations breakthroughs development. Here, we present the web (...
We describe a novel approach for screening fragments against protein that combines the sensitivity of DNA-encoded library technology with ability to explore what will bind. Each members consists fragment which is linked photoactivatable diazirine moiety. Split and pool synthesis each set linkers version reported here containing some 70k different compounds, an individual DNA code. Incubation sample followed by photoactivation, washing subsequent PCR sequencing allows hits be identified....
Abstract The abundant genetic variation harbored by wild rice ( Oryza rufipogon ) has provided a reservoir of useful genes for breeding. However, the genome not yet been comprehensively assessed. Here, we report haplotype-resolved gapless assembly and annotation Y476. In addition, develop two sets chromosome segment substitution lines (CSSLs) using Y476 as donor parent cultivated recurrent parents. By analyzing reference CSSL population, identify 254 QTLs associated with agronomic traits,...
The abounding variations in wild rice provided potential reservoirs of beneficial genes for breeding. Maintaining stable and high yields under environmental stresses is a long-standing goal breeding but challenging due to internal trade-off mechanisms. Here, we report GL12
Thylakoid biogenesis is an intricate process requiring accurate and timely assembly of proteins, pigments other cofactors into functional, photosynthetically competent membranes. PSII studied in particular as its core protein, D1, very susceptible to photodamage has a high turnover rate, particularly light. modular process, with steps proceeding specific order. Using aqueous two-phase partitioning separate plasma membranes (PM) thylakoid (TM), we the subcellular localization early for...
DNA-encoded library (DEL) technology has been used as an ultra-high-throughput screening approach for hit identification of drug targets. This process is affinity-based selection and requires incubation DEL molecules with the target. Currently, in most reported cases, input (i.e., copy number) individual varies from 105 to 107. With ever-increasing size cost, lowering while maintaining appropriate signal-to-noise ratio a paramount importance. In this article, we varied ranging 103 selections...
Abstract Background Atherosclerotic plaque vulnerability is a key feature of atheroprogression and precipitating acute cardiovascular events. Although the pivotal role epigenetic regulation in atherosclerotic destabilization being recognized, DNA methylation profile its potential driving progression disease remains largely unknown. We conducted genome-wide analysis to identify differentially methylated genes vulnerable non-vulnerable lesions understand more about pathogenesis. Results...
Inhibitor-1 (I-1), a cyclic AMP-regulated phosphoprotein, inhibits protein phosphatase-1 (PP1) activity in response to hormones. The molecular mechanism for PP1 inhibition by I-1 remains unknown. Mutation of nine acidic residues lining proposed I-1-binding channel rabbit PP1α yielded one mutant (E256A) slightly impaired its I-1, with the IC<sub>50</sub> increased 3-fold, and (E275R) located β12–β13 loop that showed 4-fold enhanced I-1. Substituting Tyr-272, binding site toxins okadaic acid...
Lung metastasis in breast cancer (BC) patients is one of the main reasons for their high mortality rate. The most prevalent BC small extracellular vesicles (sEVs receptor, integrin α6β4, has been found to interact with surfactant-associated protein (SFTPC) lung epithelial cells, making more likely metastasize lung. Tumor-associated neutrophils (TANs) play an essential role as a component pre-metastatic niche (PMN) two sides. It demonstrated that Toll-like Receptor4 (TLR4) can participate...
Abstract Congenital cataracts are the leading cause of childhood blindness. To date, surgical removal is only established treatment, but surgery associated with multiple complications, which often lead to visual impairment. Therefore, mechanistic studies and drug-candidate screening have been intrigued by aims developing novel therapeutic strategies. However, these hampered a lack an appropriate human-disease model congenital cataracts. Herein, we report establishment human cataract in vitro...
Abstract CRISPR‐Cas9 is a versatile gene editing tool with broad application of basic research and clinical therapeutics. However, the potential impact caused by off‐target effects remains critical bottleneck. The small Cas9 ortholog from Staphylococcus auricularis (SauriCas9) was identified, which recognizes 5′‐NNGG‐3′ protospacer adjacent motif (PAM), exhibiting high activity for genome editing. Recently, we also reported enhanced‐fidelity aureus (efSaCas9), harbors single mutation N260D....
In situ and ex conservation are two main wild rice ( Oryza rufipogon Griff.) protection strategies. Few studies have compared the genetic diversity structure of between in populations. Thus this study, 278 individuals collected from three nine populations Dongxiang (DXWR) were genotyped using 32 microsatellite loci to compare their population diversity. Model‐based grouping, neighbor‐joining tree, principal coordinate analyses showed that there significant differences The clustered into...
Prostate cancer is a leading malignancy in men that can also disrupt the bone tissue balance. Among all urological cancers, prostate associated with highest rate of metastases, which greatly reduce patient's quality life. In recent years, cell‑derived exosomes, contain wide range biologically active molecules, have been reported as novel method communication among individual cells. However, specific role exosomes serve this disease has not fully elucidated. The cell line PC‑3 were applied...
<h2>Summary</h2> The importance of the G-protein βγ subunits in regulation cargo transport from <i>trans</i>-Golgi network (TGN) to plasma membrane (PM) is well accepted; however, molecular mechanism underlying activation at TGN remains unclear. We show here that sphingosine 1-phosphate (S1P) receptors PM were trafficked response a surface cargo, temperature-sensitive vesicular stomatitis virus glycoprotein tagged with green fluorescent protein accumulation Golgi. receptor internalization...