A5005606848- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Single-cell and spatial transcriptomics
- dental development and anomalies
- Peptidase Inhibition and Analysis
- Shape Memory Alloy Transformations
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- Immune Response and Inflammation
- Pharmaceutical and Antibiotic Environmental Impacts
- Bone and Dental Protein Studies
- Mesenchymal stem cell research
- RNA and protein synthesis mechanisms
- Advanced Fluorescence Microscopy Techniques
- interferon and immune responses
- Composting and Vermicomposting Techniques
- Advanced Proteomics Techniques and Applications
- HIV Research and Treatment
- Circular RNAs in diseases
Sichuan University
2016-2025
Ministry of Agriculture and Rural Affairs
2024-2025
Agro-Environmental Protection Institute
2024-2025
University of California, Riverside
2018-2025
Kunming Medical University
2024-2025
Stomatology Hospital
2022-2025
State Key Laboratory of Oral Diseases
2020-2024
Ministry of Education of the People's Republic of China
2024
State Key Laboratory of Biotherapy
2018-2023
Northeast Agricultural University
2023
Significance Genome-wide identification of changes in the expression large intergenic noncoding RNAs (lincRNAs) a classical model innate immune cell activation revealed panel 159 lincRNAs that were highly modulated stimulated THP1 macrophages. One lincRNAs, named TNFα and heterogenous nuclear ribonucleoprotein L (hnRNPL) related immunoregulatory LincRNA (THRIL), was essential for induction TNFα, functions through (RNP) complex with hnRNPL, plays an important role as regulator physiological...
Although induced pluripotent stem cells (iPSCs) hold great promise for customized regenerative medicine, the molecular basis of reprogramming is largely unknown. Overcoming barriers that maintain cell identities a critical step in differentiated cells. Since microRNAs (miRNAs) modulate target genes tissue-specifically, we reasoned distinct mouse embryonic fibroblast (MEF)–enriched miRNAs post-transcriptionally proteins function as barriers. Inhibiting these should influence signaling to...
Article27 March 2019Open Access Source Data The long noncoding RNA ROCKI regulates inflammatory gene expression Qiong Zhang Department of Pediatrics, University California San Diego School Medicine, La Jolla, CA, USA Search for more papers by this author Ti-Chun Chao Veena S Patil Yue Qin Shashi Kant Tiwari Joshua Chiou orcid.org/0000-0002-4618-0647 Alexander Dobin Cold Spring Harbor Laboratory, Harbor, NY, Chih-Ming Tsai Zhonghan Li Jason Dang Shagun Gupta Kevin Urdahl Center Infectious...
Maxillofacial hard tissue defects caused by trauma or infection often affect craniofacial function. Taking the natural structure as a template, constructing an engineered repair module is important scheme to realize functional regeneration and of maxillofacial tissue. Here, inspired biomineralization process, we constructed composite mineral matrix hydrogel PAA-CMC-TDM containing amorphous calcium phosphates (ACPs), polyacrylic acid (PAA), carboxymethyl chitosan (CMC) dentin (TDM). The...
Abstract Sperm‐induced Ca 2+ rise is critical for driving oocyte activation and subsequent embryonic development, but little known about how lasting oscillations are regulated. Here it shown that NLRP14, a maternal effect factor, essential keeping early development. Few embryos lacking NLRP14 can develop beyond the 2‐cell stage. The impaired developmental potential of Nlrp14 ‐deficient oocytes mainly caused by disrupted cytoplasmic function calcium homeostasis due to altered mitochondrial...
Gene silencing by RNA triggers is an ancient, evolutionarily conserved, and widespread phenomenon. This process, known as interference (RNAi), occurs when double-stranded helices induce cleavage of their complementary mRNAs. Because these molecules can be introduced exogenously small interfering RNAs (siRNAs), RNAi has become everyday experimental tool in laboratory research. In addition, the number RNA-based therapeutics that are currently clinical trials for a variety human diseases...
Somatic cells can be reprogrammed to form embryonic stem cell-like induced pluripotent (iPSCs), but the process suffers from low efficiency and underlying molecular mechanisms that control reprogramming remain poorly understood. Here we perform an inhibitor screen identify kinases enhance, or present a barrier to, reprogramming. In particular, inhibitors of p38, inositol trisphosphate 3-kinase, Aurora A kinase potently enhance iPSC generation, iPSCs derived inhibitor-treated somatic are...
With a dataset of more than 600 million small RNAs deeply sequenced from mouse hippocampal and staged sets cells that underwent reprogramming to induced pluripotent stem cells, we annotated the stem-loop precursors known miRNAs identify isomoRs (miRNA-offset RNAs), loops, non-preferred strands, guide strands. Products both strands were readily detectable for most miRNAs. Changes in dominant isomiR occurred among cell types, as did switches preferred strand. The terminal nucleotide aligned...
Dental pulp is critical to maintain the vitality of a tooth. Regeneration pulpo-dentinal complex great interest treat pulpitis and necrosis. In this study, through three-dimensional spheroid culture, group unique multipotent stem cells were identified from mouse dental papilla called regenerative (MDPSCs). MDPSCs exhibited enhanced osteogenic/odontogenic differentiation capabilities could form dentin neurovascular-like structures that mimicked native teeth in vivo. Further analysis revealed...
A massive intronic hexanucleotide repeat (GGGGCC) expansion in C9ORF72 is a genetic origin of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Recently, C9ORF72, together with SMCR8 WDR41, has been shown to regulate autophagy function as Rab GEF. However, the precise remains unclear. Here, we report cryogenic electron microscopy (cryo-EM) structure human C9ORF72-SMCR8-WDR41 complex at resolution 3.2 Å. The reveals dimeric assembly heterotrimer C9ORF72-SMCR8-WDR41....
Despite our increased understanding of the functions lncRNAs, their potential to develop HIV/AIDS cure strategies remains unexplored. A genome-wide analysis lncRNAs in HIV-1-infected primary monocyte-derived macrophages (MDMs) was performed, and 1,145 differentially expressed were identified. An lncRNA named H IV-1- e nh a nced l ncRNA ( HEAL ) is upregulated by HIV-1 infection promotes HIV replication T cells macrophages. forms complex with RNA-binding protein FUS enhance transcriptional...
Abnormal activation of epidermal growth factor receptor (EGFR) drives non-small cell lung cancer (NSCLC) development. EGFR mutations-mediated resistance to tyrosine-kinase inhibitors (TKIs) is a major hurdle for NSCLC treatment. Here, we show that F-box protein FBXL2 targets and TKI-resistant mutants proteasome-mediated degradation, resulting in suppression EGFR-driven growth. Reduced expression associated with poor clinical outcomes patients. Furthermore, glucose-regulated 94 (Grp94)...
It′s a TRAP! microRNAs (miRNAs) are noncoding small RNAs that consist of approximately 21 nucleotides and assembled into an RNA-induced silencing complex (RISC), which suppresses target mRNA expression. An easy-to-use method called miRNA RNA affinity purification (miR-TRAP) directly identifies targets in vivo. This could be widely used to discover various disease models under physiological conditions.
The innate immune system is the first line of defense against microbial pathogens, but tight regulation gene expression necessary to prevent detrimental effects unrestrained activation. Although functions most long noncoding RNAs (lncRNAs; >200 nucleotides) are unknown, many have been shown regulate diverse cellular activities. Recent reports by us and others suggested that lncRNAs may also play critical roles in transcriptional during responses. Following engagement Toll-like receptors,...