Superhydrophobic surfaces (SHS) offer versatile applications by trapping an air layer within microstructures, while water jet impact can destabilize this and deactivate the functions of SHS. The current work presents for first time that introducing parallel hydrophilic strips to SHS (SHS-s) simultaneously improve both impalement resistance drag reduction (DR). Compared with SHS, SHS-s demonstrates a 125% increase in enduring against velocity 11.9 m/s 97% improvement DR at Reynolds number 1.4...

Intervertebral disc degeneration (IVDD) is a primary contributor to low back pain and poses considerable burden society. However, the molecular mechanisms underlying IVDD remain be elucidated. PR/SET domain 1 (PRDM1) regulates cell proliferation, apoptosis, inflammatory responses in various diseases. Despite these regulatory functions, mechanism of action PRDM1 remains unexplored. In this study, we investigated role progression. The expression nucleus pulposus (NP) tissues NP cells (NPCs)...

Hypertrophy of the ligamentum flavum (LF) is one key pathomechanisms lumbar spinal stenosis (LSS). Transforming growth factor (TGF)-β1 abundantly expressed in hypertrophied degenerative LF tissues from LSS. However, molecular mechanisms underling association between TGF-β1 and hypertrophy have not yet been fully elucidated. In this study, we investigated important role mitogen-activated protein kinase (MAPK) pathway pathogenesis LSS by analyzing expression connective tissue (CTGF)...

Superhydrophobic surfaces hold immense potential in underwater drag reduction. However, as the Reynolds number increases, reduction rate decreases, and it may even lead to a increase. The reason lies collapse of air mattress. To address this issue, paper develops pyramid-shaped robust superhydrophobic surface with wedged microgrooves, which exhibits high gas fraction when immersed good ability achieve complete spreading recovery mattress through replenishment case Pressure drop tests water...

Abstract Background Ligamentum flavum (LF) hypertrophy is the main cause of lumbar spinal canal stenosis (LSCS). Previous studies have shown that LF tissue exhibits abnormal lipid accumulation, but regulatory mechanism remains unclear. The objective this study was to explore function and potential ACSM5 in accumulation. Methods To assess expression levels, accumulation triglyceride (TG) level normal tissue, we utilized RT-qPCR, western blot, oil red O staining, TG assay kit. pearson...

Abstract Background Increasing evidences have shown that long non-coding RNAs (lncRNAs) display crucial regulatory roles in the occurrence and development of numerous diseases. However, function underlying mechanisms lncRNAs hypertrophy ligamentum flavum (HLF) not been report. Methods The integrated analysis sequencing, bioinformatics real-time quantitative PCR were used to identify key involved HLF progression. Gain- loss-function experiments explore functions lncRNA X inactive specific...

A dissection-based study of 6 embalmed cadavers.To identify and describe the extraforaminal ligaments (EFLs) in relation to area cervical intervertebral foramina evaluate their clinical significance.EFLs between lumbar spinal nerves tissues surrounding foramens have been well established. However, research work has undertaken local anatomy part spine; detailed anatomic studies EFLs not performed.One hundred ninety-six from adult cadavers were studied, existence type identified. The...

Abstract Background Hypertrophy of ligamentum flavum (HLF) is the mainly cause lumbar spinal stenosis (LSS), but precise mechanism HLF formation has not been fully elucidated. Emerging evidence indicates that transcription factor 7 (TCF7) key downstream functional molecule Wnt/β-catenin signaling, which participated in regulating multiple biological processes. However, role and underlying TCF7 still unclear. Methods We used mRNAs sequencing analysis human LF subsequent confirmation with...

We describe and characterize a series of polyclonal antibodies, generated against amino acid sequences unique to various regions within pro- mature brain-derived neurotrophic factor (BDNF), member the highly conserved nerve growth (NGF) family neurotrophins. Synthetic peptides were coupled carrier proteins in presence glutaraldehyde restrict host animals' immune response epitopes that are compatible with aldehyde fixation. Initial screenings reactivity antisera made on brain sections...

Abstract Background Hypertrophy of ligamentum flavum (HLF) is a common lumbar degeneration disease (LDD) with typical symptoms low back pain and limb numbness owing to an abnormal pressure on spinal nerves. Previous studies revealed HLF might be caused by fibrosis, inflammatory, other bio-pathways. However, global analysis needed severely. Methods A genome-wide DNA methylation single-nucleotide polymorphism were performed from five LDD patients without HLF. Comprehensive integrated was using...

Background Medical disputes are common in hospitals and a major challenge for the operations of medical institutions. However, few studies have looked into association between hospital legal constructions. The purpose study was to investigate relationship constructions disputes, it also aimed develop nomogram estimate likelihood disputes. Methods Between July September 2021, 2,716 administrators from 130 were enrolled analysis. collected seventeen variables examination. To establish...

In physiological and pathological environments, the concentration of oxygen around osteoblasts varies widely. No studies have systematically evaluated effects different concentrations on proliferation, survival, migration, osteogenic differentiation osteoblasts. this study, we cultured osteoblast precursor cell line MC3T3-E1 in small individual chambers with 1%, 3%, 6%, 9%, 21%. Cell proliferation was by index test EdU staining. To a live/dead assay performed. A tablet scratch performed to...

To investigate the mechanism of p38 mitogen activated protein kinase (MAPK) signaling pathway in regulating hyperplasia and hypertrophy human lumbar ligamentum flavum via transforming growth factor β 1 (TGF-β 1)/connective tissue (CTGF).The taken from patient with intervertebral disc herniation was isolated by collagenase-predigested explant cultures. The cells were treated extracellular regulated blocker PD98059, c-Jun N-terminal SP600125, SB203580, then mRNA expressions CTGF, collagen type...

Prostate cancer is a leading malignancy in men that can also disrupt the bone tissue balance. Among all urological cancers, prostate associated with highest rate of metastases, which greatly reduce patient's quality life. In recent years, cell‑derived exosomes, contain wide range biologically active molecules, have been reported as novel method communication among individual cells. However, specific role exosomes serve this disease has not fully elucidated. The cell line PC‑3 were applied...

<title>Abstract</title> <bold>Background:</bold> Hepatocellular carcinoma (HCC) accounts for approximately 90% of primary liver cancers and is charactered by poor prognosis. The identification potential prognostic biomarkers essential HCC patients. <bold>Methods: </bold>In this study, Gene Expression Profiling Interative Analysis (GEPIA) online tool was used to the different expression TRIP13 has-miR-29c-3p axis between tumor samples normal in multiple including hepatocellular (HCC)....

To investigate the effect of transforming growth factor β 1 (TGF-β 1) induced proliferation ligamentum flavum cells and hypertrophy its on connective tissue (CTGF) expression.The in lumbar intervertebral disc herniation was extracted were isolated cultured by collagenase pre-digestion method. Morphological observation, immunofluorescence staining MTT assay used for cell identification. The 3rd generation divided into 5 groups. groups A, B, C, D respectively sealed with 3 ng/mL TGF-β 1, 50...

Soft actuators have a wide range of applications in medical instruments, soft robotics, 3D electronics, and deployable structures, where configuration transitions are crucial for their function realization. However, most can only morph from the initial directly to final under single external stimulus. Herein, we report novel actuator by printing parallel strips with crescent cross-sections onto thin PDMS film. Multiple observed when swells ethyl acetate. Four factors, i.e., geometric...