A5032511920- Pluripotent Stem Cells Research
- Hippo pathway signaling and YAP/TAZ
- CRISPR and Genetic Engineering
- Medical Malpractice and Liability Issues
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Healthcare cost, quality, practices
- Dispute Resolution and Class Actions
- Monoclonal and Polyclonal Antibodies Research
- Kruppel-like factors research
- Phosphodiesterase function and regulation
- Wnt/β-catenin signaling in development and cancer
- Motor Control and Adaptation
- Iron Metabolism and Disorders
- Transcranial Magnetic Stimulation Studies
- Developmental Biology and Gene Regulation
- Advancements in Transdermal Drug Delivery
- Geology and Paleoclimatology Research
- Neonatal Health and Biochemistry
- China's Socioeconomic Reforms and Governance
- Viral Infectious Diseases and Gene Expression in Insects
- Genetics, Aging, and Longevity in Model Organisms
- Neurological Disease Mechanisms and Treatments
- Healthcare Quality and Management
- Healthcare Systems and Reforms
Army Medical University
2020-2024
Chongqing Medical University
2024
China-Japan Friendship Hospital
2014-2023
Army Medical College
2023
Southwest Hospital
2023
Yueyang Hospital
2022
Shanghai University of Traditional Chinese Medicine
2022
Weihai Municipal Hospital
2022
Chinese Academy of Sciences
2020
Center for Excellence in Molecular Cell Science
2020
The carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) poses a severe therapeutic challenge to global public health, and research on CR-hvKP in older patients remain limited. In this study, we aimed investigate the clinical molecular characteristics risk factors of infections patients. We retrospectively investigated with (CRKP) intensive care unit (ICU) between January 2020 December 2020. data, microbiological data including antimicrobial susceptibility testing, phenotype...
The incidence of patient-doctor disputes are alarmingly increasing in China, this article reviews the current status and causes violence against medical workers six strategies to tackle daily worrying problems have been proposed hopefully could improve working environment China.
Medical disputes are a global public health issue that is receiving increasing attention. However, studies investigating the relationship between hospital legal construction and medical scarce. The development of multicenter model incorporating machine learning (ML) techniques for individualized prediction would be beneficial workers.This study aimed to identify predictors related from perspective use ML build models predicting risk disputes.This enrolled 38,053 workers 130 tertiary...
Induction of broadly neutralizing monoclonal antibodies (bNAbs) that bind to the viral envelope glycoproteins is a major goal hepatitis C virus (HCV) vaccine research. The study bNAbs arising in natural infection essential this endeavor. We generated human antibody, 8D6, recognizing E2 protein HCV isolated from chronic patient. This antibody shows activity, which covers pan-genotypic panel cell culture-derived virions (HCVcc). Functional and epitope analyses demonstrated 8D6 can block...
Abstract A comprehensive and precise definition of the pluripotency gene regulatory network (PGRN) is crucial for clarifying mechanisms in embryonic stem cells (ESCs). Here, after a CRISPR/Cas9-based functional genomics screen integrative analysis with other genomes, transcriptomes, proteomes epigenome data, an expanded pluripotency-associated set obtained, new PGRN nine sub-classes constructed. By integrating DNA binding, epigenetic modification, chromatin conformation, RNA expression...
Stearic acid-grafted chitosan (CS-SA) and glycyrrhetinic acid-conjugated stearic (GA-CS-SA) were synthesized further used for the preparation of micelles. The substitution degree (SD) SA GA on CS was measured. physicochemical properties CS-SA GA-CS-SA micelles such as critical micelle concentration (CMC), aggregation number hydrophobic micro-domain (AN), particle size, zeta potential, morphology also determined. CMC about 17.49 μg/mL, which relatively low. Its AN 2.09. showed spherical shape...
Optical coherence tomography (OCT) allows noncontact acquisition of fingerprints and hence is a highly promising technology in the field biometrics. OCT can be used to acquire both structural microangiographic images fingerprints. Microangiographic derives its contrast from blood flow vasculature viable skin tissue, fingerprint imaging inherently immune fake attack. Therefore, dual-modality (structural microangiographic) will enable more secure biometric data, which has not been investigated...
Background Medical disputes are common in hospitals and a major challenge for the operations of medical institutions. However, few studies have looked into association between hospital legal constructions. The purpose study was to investigate relationship constructions disputes, it also aimed develop nomogram estimate likelihood disputes. Methods Between July September 2021, 2,716 administrators from 130 were enrolled analysis. collected seventeen variables examination. To establish...
Yap is the key effector of Hippo signaling; however, its role in embryonic stem cells (ESCs) remains controversial. Here, we identify two splicing isoforms (Yap472 and Yap488), which show equal expression levels but heterogeneous distribution ESCs. Knockout (KO) both reduces ESC self-renewal, accelerates pluripotency exit, arrests terminal differentiation, while overexpression each isoform leads to reverse phenotype. The effect on self-renewal Teads-dependent mediated by c-Myc. Nonetheless,...
Dax1(also known as Nr0b1) is regarded an important component of the transcription factor network in mouse embryonic stem cells (ESCs). However, role and molecular mechanism Dax1 maintenance different pluripotency states are poorly understood. Here, we constructed a stable knockout (KO) cell line using CRISPR/Cas9 system to analyze precise function Dax1. We reported that 2i/LIF-ESCs had significantly lower expression than LIF/serum-ESCs. Dax1KO ES lines could be established 2i/LIF their was...
To study the transition from Paleolithic to Neolithic period and its duration, samples of charcoal, bone, flowstone shells excavated Bailiandong Miaoyan caves, Guangxi Zhuang autonomous region, South China were dated using Peking University AMS facility liquid scintillation counter. The remains these sites show typical characteristics Neolithic. Radiocarbon dating results a rapid ca. 20 10 ka bp.
The mechanisms underlying self-renewal of embryonic stem cells (ESCs) hold great value in the clinical translation cell biology and regenerative medicine research. To study ESC self-renewal, screening identification key genes maintaining were performed by a genome-wide CRISPR-Cas9 knockout virus library. mouse R1 infected with library cultured for 14 days. variation single guide RNA (sgRNA) ratio was analyzed high-throughput sequencing, followed bioinformatics analysis to profile altered...
It is important to systematically identify tumor suppressor genes (TSGs) improve our understanding of tumorigenesis and develop strategies for early diagnosis mitigating disease progression. In the present study, we used an in vivo genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) screen identified FPS/FES-related (FER) as a TSG. Single-cell RNA sequencing (scRNA-seq) revealed that normal cells with low FER expression exhibited...
Hippo signaling pathway is involved in many biological processes including the fate decision of embryonic stem cells (ESCs). Yes-associated protein (Yap) function as a key effector pathway, but its role ESCs still controversial. So far, only two isoforms Yap have been identified and they both overlapping distinct functions. Here, we identify six novel mouse Yap, bringing total number to eight. According differences first exon, are divided into subtypes (a b). Isoform-a isoform-b exhibit...
The pluripotency of embryonic stem cells (ESCs) is more accurately viewed as a continuous developmental process rather than fixed state. However, the factors that play general or state-specific roles in regulating self-renewal different states remain poorly defined. In this study, parallel genome-wide CRISPR/Cas9 knockout (KO) screens were applied ESCs cultured serum plus LIF (SL) and 2i (2iL) conditions. candidate genes classified into seven groups based on their positive negative effects...
Abstract A comprehensive and precise definition of the pluripotency gene regulatory network (PGRN) is crucial for clarifying mechanisms in embryonic stem cells (ESCs). Here, after a CRISPR/Cas9-based functional genomics screen integrative analysis with other genomes, transcriptomes, proteomes epigenome data, an expanded pluripotency-associated set obtained, new PGRN nine sub-classes constructed. By integrating DNA binding, epigenetic modification, chromatin conformation, RNA expression...
As a key effector of the Hippo pathway, yes-associated protein (YAP) is major regulator cell proliferation and apoptosis. In this study, 23 hYAP isoforms were identified in HEK293 cells, with 14 being reported for first time. These classified into hYAP-a hYAP-b based on variation exon 1. The two groups showed distinctly different subcellular localizations. could activate TEAD- or P73-mediated transcription, affect rate, enhance cellular chemosensitivity cells. Moreover, activation abilities...
Dax1 (Nr0b1; Dosage-sensitive sex reversal-adrenal hypoplasia congenital on the X-chromosome gene-1) is an important component of transcription factor network that governs pluripotency in mouse embryonic stem cells (ESCs). Functional evaluation alternative splice variants pluripotent factors has shed additional insight maintenance ESC and self-renewal. have not been identified characterized ESCs. We 18 new transcripts with putative protein-coding properties compared their protein structures...