A5017590136- Helicobacter pylori-related gastroenterology studies
- Galectins and Cancer Biology
- Viral Infections and Immunology Research
- Cellular transport and secretion
- Cell Adhesion Molecules Research
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- RNA modifications and cancer
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Enzyme Structure and Function
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- Folate and B Vitamins Research
- Biochemical and Structural Characterization
- Immune Response and Inflammation
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Porphyrin Metabolism and Disorders
- HIV/AIDS drug development and treatment
- Microtubule and mitosis dynamics
- RNA regulation and disease
- Lipid Membrane Structure and Behavior
Hainan Medical University
2020-2025
Zhengzhou University
2014-2024
Guangdong University of Technology
2024
Shanghai University of Traditional Chinese Medicine
2023-2024
Chengdu University of Technology
2020-2024
China University of Mining and Technology
2024
Center for Excellence in Molecular Cell Science
2014-2021
Chinese Academy of Sciences
2012-2021
University of Chinese Academy of Sciences
2017-2021
Institute of Biophysics
2011-2020
The structural basis for the divalent cation–dependent binding of heterodimeric αβ integrins to their ligands, which contain prototypical Arg-Gly-Asp sequence, is unknown. Interaction with ligands triggers tertiary and quaternary rearrangements in that are needed cell signaling. Here we report crystal structure extracellular segment integrin αVβ3 complex a cyclic peptide presenting sequence. ligand binds at major interface between αV β3 subunits makes extensive contacts both. Both changes...
Integrins are αβ heterodimeric receptors that mediate divalent cation-dependent cell-cell and cell-matrix adhesion through tightly regulated interactions with ligands. We have solved the crystal structure of extracellular portion integrin αVβ3 at 3.1 Å resolution. Its 12 domains assemble into an ovoid “head” two “tails.” In crystal, is severely bent a defined region in its tails, reflecting unusual flexibility may be linked to regulation. The main inter-subunit interface lies within head,...
Significance Proofreading exonucleases contributing to replication fidelity in DNA viruses and cellular organisms are well known; however, proofreading RNA was unknown until recently. Coronavirus nonstructural protein 14 (nsp14) has been shown function as a exoribonuclease. Additionally, nsp14 shows (guanine-N7) methyl transferase activity for viral mRNA capping. Both roles important transcription. Here, we report the structures of severe acute respiratory syndrome-coronavirus complex with...
The crystal structure of a complex involving the D10 T cell receptor (TCR), 16-residue foreign peptide antigen, and I-Ak self major histocompatibility (MHC) class II molecule is reported at 3.2 angstrom resolution. TCR oriented in an orthogonal mode relative to its peptide-MHC (pMHC) ligand, necessitated by amino-terminal extension residues projecting from MHC antigen-binding groove as part mini beta sheet. Consequently, disposition complementarity-determining region loops altered that most...
The clinical manifestations of cholera are largely attributable to the actions a secreted hexameric AB5enterotoxin (choleragen). We have independently solved and refined three-dimensional structure choleragen at 2.5 Å resolution. crystalline toxin closely resembles that described for heat-labile enterotoxin fromEscherichia coli(LT) with which it shares 80% sequence homology. In both cases, wedge-shaped A subunit is loosely held high above plane pentameric B subunits by tethering A2 chain....
Thrombospondin-1 (TSP-1) contains three type 1 repeats (TSRs), which mediate cell attachment, glycosaminoglycan binding, inhibition of angiogenesis, activation TGFβ, and matrix metalloproteinases. The crystal structure the TSRs reported in this article reveals a novel, antiparallel, three-stranded fold that consists alternating stacked layers tryptophan arginine residues from respective strands, capped by disulfide bonds on each end. front face TSR right-handed spiral, positively charged...
The structural basis of the interaction between CD4 coreceptor and a class II major histocompatibility complex (MHC) is described. crystal structure containing human N-terminal two-domain fragment murine I-A(k) MHC molecule with associated peptide (pMHCII) shows that only "top corner" directly contacts pMHCII. Phe-43 side chain extends into hydrophobic concavity formed by residues from both alpha 2 beta domains. A ternary model CD4-pMHCII-T-cell receptor (TCR) reveals appears V-shaped...
Heme is a ligand for the human nuclear receptors (NR) REV-ERBalpha and REV-ERBbeta, which are transcriptional repressors that play important roles in circadian rhythm, lipid glucose metabolism, diseases such as diabetes, atherosclerosis, inflammation, cancer. Here we show transcription repression mediated by heme-bound REV-ERBs reversed addition of nitric oxide (NO), heme NO effects C-terminal ligand-binding domain (LBD). A 1.9 crystal structure REV-ERBbeta LBD, complex with oxidized Fe(III)...
Significance Bunyaviruses are emerging zoonotic pathogens of public-health concern. Lack structures for proteins on the viral membrane (“envelope”) surface limits understanding entry. We describe atomic-level globular “head” envelope protein, glycoprotein N (Gn), from two members, severe fever with thrombocytopenia syndrome virus (SFTSV) and Rift Valley (RVFV), Phleboviruses genus in bunyavirus family, a structure SFTSV Gn bound neutralizing antibody Fab. The results show folded define...
The transition of the Munc18-1/syntaxin-1 complex to SNARE complex, a key step involved in exocytosis, is regulated by Munc13-1, SNAP-25 and synaptobrevin-2, but underlying mechanism remains elusive. Here, we identify an interaction between Munc13-1 membrane-proximal linker region reveal its essential role exocytosis. Upon this interaction, not only recruits synaptobrevin-2-embedded vesicles target membrane also renders synaptobrevin-2 motif more accessible complex. Afterward, entry leads...
Abstract Mammalian shelterin proteins POT1 and TPP1 form a stable heterodimer that protects chromosome ends regulates telomerase-mediated telomere extension. However, how interacts with remains unknown. Here we present the crystal structure of C-terminal portion human (POT1C) complexed POT1-binding motif TPP1. The shows POT1C contains two domains, third OB fold Holliday junction resolvase-like domain. Both domains are essential for binding to Notably, unlike heart-shaped ciliated protozoan...
IMP dehydrogenase (IMPDH) is an essential enzyme that catalyzes the first step unique to GTP synthesis. To provide a basis for evaluation of IMPDH inhibitors as antimicrobial agents, we have expressed and characterized from pathogenic bacterium Streptococcus pyogenes. Our results show biochemical kinetic characteristics S. pyogenes are similar other bacterial enzymes. However, lack sensitivity mycophenolic acid Km NAD (1180 μM) exemplify some differences between mammalian enzymes, making it...
Heme-degrading enzymes are involved in human diseases ranging from stroke, cancer, and multiple sclerosis to infectious such as malaria, diphtheria, meningitis. All mammalian microbial identified date members of the heme oxygenase superfamily assume similar monomeric structures with an all alpha-helical fold. Here we describe crystal IsdG IsdI, two heme-degrading Staphylococcus aureus. The both resemble ferredoxin-like fold form a beta-barrel at dimer interface. Two large pockets found on...
The transformation potential of Simian Virus 40 depends on the activities large T-antigen (LTag), which interacts with several cellular tumor suppressors including important “guardian” genome, p53. Inhibition p53 function by LTag is necessary for both efficient viral replication and transformation. We determined crystal structure in complex reveals an unexpected hexameric binding six monomers. Structure-guided mutagenesis residues supported p53–LTag interface defined structure. also shows...