A5003012923- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- Cancer-related gene regulation
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Nuclear Structure and Function
- Histone Deacetylase Inhibitors Research
- Hemoglobin structure and function
- Fungal and yeast genetics research
- Advanced Measurement and Metrology Techniques
- Manufacturing Process and Optimization
- Immunotherapy and Immune Responses
- Porphyrin Metabolism and Disorders
- Ubiquitin and proteasome pathways
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Mass Spectrometry Techniques and Applications
- Cancer-related molecular mechanisms research
Shanghai Ninth People's Hospital
2017-2024
Shanghai Jiao Tong University
2008-2024
South China University of Technology
2021
State Key Laboratory of Oncogene and Related Genes
2020-2021
Zhejiang University
2021
Zhengzhou University
2021
University of California, San Diego
2021
Army Medical University
2021
Guizhou University
2021
Affiliated Hospital of Southwest Medical University
2021
Abstract Flavonoids are the major functional components of many herbal and insect preparations demonstrate varied pharmacological functions including antibacterial activity. Here by enzymatic assay crystal structure analysis, we studied inhibition three flavonoids (quercetin, apigenin, (S)‐sakuranetin) against β‐hydroxyacyl‐acyl carrier protein dehydratase from Helicobacter pylori (HpFabZ). These all competitive inhibitors HpFabZ either binding to entrance substrate tunnel B (binding model...
Recent studies indicate that histones are subjected to various types of acylation including acetylation, propionylation and crotonylation. CBP p300 have been shown catalyze multiple but not conserved in evolution, raising the question as existence other enzymes for histone functional relationship between well-characterized acetylation acylation. In this study, we focus on catalyzing crotonylation demonstrate among known acetyltransferases, MOF, addition p300, also possesses...
SLX4 interacts with several endonucleases to resolve structural barriers in DNA metabolism. also telomeric protein TRF2 human cells. The molecular mechanism of these interactions at telomeres remains unknown. Here, we report the crystal structure TRF2-binding motif (SLX4TBM) complex TRFH domain (TRF2TRFH) and map SLX1, XPF, MUS81. recognizes a unique HxLxP on via peptide-binding site its domain. Telomeric localization associated nucleases depend SLX4-endonuclease SLX4-TRF2 levels TRF2....
Abstract Mammalian shelterin proteins POT1 and TPP1 form a stable heterodimer that protects chromosome ends regulates telomerase-mediated telomere extension. However, how interacts with remains unknown. Here we present the crystal structure of C-terminal portion human (POT1C) complexed POT1-binding motif TPP1. The shows POT1C contains two domains, third OB fold Holliday junction resolvase-like domain. Both domains are essential for binding to Notably, unlike heart-shaped ciliated protozoan...
Abstract NDH-1 is a key component of the cyclic-electron-transfer around photosystem I (PSI CET) pathway, an important antioxidant mechanism for efficient photosynthesis. Here, we report 3.2-Å-resolution cryo-EM structure ferredoxin (Fd)-NDH-1L complex from cyanobacterium Thermosynechococcus elongatus . The reveals three β-carotene and fifteen lipid molecules in membrane arm NDH-1L. Regulatory oxygenic photosynthesis-specific (OPS) subunits NdhV, NdhS NdhO are close to Fd-binding site whilst...
Ribonuclease P (RNase P) is a universal ribozyme responsible for processing the 5'-leader of pre-transfer RNA (pre-tRNA). Here, we report 3.5-angstrom cryo-electron microscopy structures Saccharomyces cerevisiae RNase alone and in complex with pre-tRNAPhe The protein components form hook-shaped architecture that wraps around stabilizes into "measuring device" two fixed anchors recognize L-shaped pre-tRNA. A universally conserved uridine nucleobase phosphate backbone catalytic center together...
Abstract Intraflagellar transport (IFT) trains, the polymers composed of two multi-subunit complexes, IFT-A and IFT-B, carry out bidirectional intracellular in cilia, vital for cilia biogenesis signaling. plays crucial roles ciliary import membrane proteins retrograde cargo trafficking. However, molecular architecture assembly mechanism into IFT trains vivo remains elusive. Here, we report cryo-electron microscopic structures complex from protozoa Tetrahymena thermophila . We find that...
Adapted to the task at hand RNA-based catalysts perform fundamental tasks in cellular RNA metabolism, especially eukaryotes, where RNAs are cut by specialized ribonucleoproteins (RNPs) as part of ribosome assembly or messenger regulation splicing. Both and protein components play a role shaping how these large catalytic complexes interact with their substrates. Lan et al. determined cryo–electron microscopy structures yeast RNP called ribonuclease MRP both alone bound small substrate....
Abstract Telomerase, a multi-subunit ribonucleoprotein complex, is unique reverse transcriptase that catalyzes the processive addition of repeat sequence to extend telomere end using short fragment its own RNA component as template. Despite recent structural characterizations human and Tetrahymena telomerase, it still mystery how telomerase repeatedly uses template synthesize telomeric DNA. Here, we report cryo-EM structure holoenzyme bound with DNA at resolutions 3.5 Å 3.9 for catalytic...
Abstract EDA is a tumor necrosis factor (TNF) family member, which functions together with its cognate receptor EDAR during ectodermal organ development. Mutations of have long been known to cause X‐linked hypohidrotic dysplasia in humans characterized by primary defects teeth, hair and sweat glands. However, the structural information interaction lacking pathogenic mechanism variants poorly understood. Here, we report crystal structure C-terminal TNF homology domain bound N-terminal...
Examination of factors regulating oocyte chromatin remodeling is crucial to circumvent embryonic aneuploidy and resulting defects. Aurora kinases (AURK) are involved in regulation remodeling, however, little attention has been paid AURKs regard maturation. Meiotically incompetent mouse oocytes contain transcripts for all three Aurk isoforms: A, B C. Upon achieving meiotic competence, showed significant increases transcript levels isoforms remained unchanged as progressed through meiosis,...
Dendritic cells (DCs) are key mediators of immune function through robust and tightly regulated presentation antigen in the context MHC Class II. II expression is controlled by transactivator CIITA. CIITA conventional DCs uniquely dependent on an uncharacterized myeloid cell-specific promoter, CIITApI. We now identify vivo promoter structure factors regulating In immature transcription requires binding PU.1, IRF8, NFκB, Sp1 to promoter. PU.1 binds independently at one site a required...
Abstract During meiotic prophase I, telomeres attach to and move on the nuclear envelope (NE), regulating chromosome movement promote homologous pairing. Meiosis-specific proteins TERB1, TERB2 MAJIN play a key role in this process. Here, we report crystal structures of human TERB1-TERB2 TERB2-MAJIN subcomplexes. Specific disruption or interaction mouse Terb2 gene abolishes telomere attachment NE causes aberrant pairing disordered synapsis. In addition, depletion SUN1 also partially disrupts...
Abstract Ribonuclease P (RNase P) is an essential ribozyme responsible for tRNA 5′ maturation. Here we report the cryo-EM structures of Methanocaldococcus jannaschii ( Mja ) RNase holoenzyme alone and in complex with a substrate at resolutions 4.6 Å 4.3 Å, respectively. The reveal that subunits are strung together to organize dimeric conformation required efficient catalysis. also show archaeal functional chimera bacterial eukaryal Ps possesses bacterial-like two RNA-based anchors...