A5074181295- Telomeres, Telomerase, and Senescence
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- dental development and anomalies
- Nuclear Structure and Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Genetic and Mutation Studies
- Wnt/β-catenin signaling in development and cancer
- Genetic and Kidney Cyst Diseases
- Hemoglobin structure and function
- Advanced Memory and Neural Computing
- Neuroscience and Neural Engineering
- Nutrition and Health in Aging
- Photoreceptor and optogenetics research
- Perfectionism, Procrastination, Anxiety Studies
- Reproductive Biology and Fertility
- Connexins and lens biology
- Dysphagia Assessment and Management
- Orthopaedic implants and arthroplasty
Shanghai Jiao Tong University
2015-2024
Shanghai Ninth People's Hospital
2017-2024
Wenzhou Medical University
2023
Kitano Hospital
2023
Peking University
2003-2022
Guigang City People's Hospital
2018
Washington State University Spokane
2010-2017
Georgia Highlands College
2016-2017
Uppsala University
2007-2013
Washington State University
2010-2012
The telomeric CTC1/STN1/TEN1 (CST) complex has been implicated in promoting replication recovery under stress at genomic regions, yet its precise role is unclear. Here, we report that STN1 enriched GC-rich repetitive sequences genome-wide response to hydroxyurea (HU)-induced stress. deficiency exacerbates the fragility of these stress, resulting chromosome fragmentation. We find upon fork stalling, CST proteins form distinct nuclear foci colocalize with RAD51. Furthermore, induces physical...
During meiosis, chromosomes exhibit dramatic changes in morphology and intranuclear positioning. How these influence homolog pairing, alignment, recombination remain elusive. Using Hi-C, we systematically mapped 3D genome architecture throughout all meiotic prophase substages during mouse spermatogenesis. Our data uncover two major chromosome organizational features varying along the axis early prophase, when alignment occurs. First, transcriptionally active inactive genomic regions form...
Abstract Intraflagellar transport (IFT) trains, the polymers composed of two multi-subunit complexes, IFT-A and IFT-B, carry out bidirectional intracellular in cilia, vital for cilia biogenesis signaling. plays crucial roles ciliary import membrane proteins retrograde cargo trafficking. However, molecular architecture assembly mechanism into IFT trains vivo remains elusive. Here, we report cryo-electron microscopic structures complex from protozoa Tetrahymena thermophila . We find that...
Teratozoospermia is usually associated with defective spermiogenesis and a disorder considerable genetic heterogeneity. Although previous studies have identified several teratozoospermia-associated genes, the etiology remains unknown for majority of affected men. Here, we homozygous missense mutation compound heterozygous CCIN in patients suffering from teratozoospermia. encodes cytoskeletal protein Calicin that involved formation maintenance highly regular organization calyx mammalian...
Abstract EDA is a tumor necrosis factor (TNF) family member, which functions together with its cognate receptor EDAR during ectodermal organ development. Mutations of have long been known to cause X‐linked hypohidrotic dysplasia in humans characterized by primary defects teeth, hair and sweat glands. However, the structural information interaction lacking pathogenic mechanism variants poorly understood. Here, we report crystal structure C-terminal TNF homology domain bound N-terminal...
Background6-Aminophenanthridine (6AP) and Guanabenz (GA, a drug currently in use for the treatment of hypertension) were isolated as antiprion drugs using yeast-based assay. These structurally unrelated molecules are also active against mammalian prion several cell-based assays vivo mouse model prion-based diseases.Methodology/Principal FindingsHere we report identification cellular targets these drugs. Using affinity chromatography matrices both drugs, demonstrate an RNA-dependent...
Abstract During meiotic prophase I, telomeres attach to and move on the nuclear envelope (NE), regulating chromosome movement promote homologous pairing. Meiosis-specific proteins TERB1, TERB2 MAJIN play a key role in this process. Here, we report crystal structures of human TERB1-TERB2 TERB2-MAJIN subcomplexes. Specific disruption or interaction mouse Terb2 gene abolishes telomere attachment NE causes aberrant pairing disordered synapsis. In addition, depletion SUN1 also partially disrupts...
Abstract Chromosomes pair and synapse with their homologous partners to segregate correctly at the first meiotic division. Association of telomeres LINC (Linker Nucleoskeleton Cytoskeleton) complex composed SUN1 KASH5 enables telomere-led chromosome movements telomere bouquet formation, facilitating precise pairwise alignment homologs. Here, we identify a direct interaction between Speedy A (SPDYA) determine crystal structure human SUN1-SPDYA-CDK2 ternary complex. Analysis meiosis prophase I...
Termination of messenger RNA translation in Bacteria and Archaea is initiated by release factors (RFs) 1 or 2 recognizing a stop codon the ribosomal A site releasing peptide from P-site transfer RNA. After release, RF-dissociation facilitated G-protein RF3. Structures complexes with RF1 RF2 alone RF3 alone—RF3 bound to non-hydrolyzable GTP-analog—have been reported. Here, we present cryo-EM structure post-termination ribosome containing both apo-RF3 RF1. The conformation distinct those free...
Abstract Dysphagia is a side effect of nasopharyngeal carcinoma chemo-radiotherapy (CRT) which greatly influences the quality life patients. We analyzed late dysphagia in 134 patients with cancer undergoing radical radiotherapy (RT), and correlated these findings dose–volume histogram (DVH) parameters swallowing organs at risk (SWOARs). DVH SWOARs were dysphagia, RTOG/EORTC scale score M. D. Anderson inventory (MDADI) score. The mean dose (D ) to superior inferior constrictor muscles (SCM...
This study aimed to investigate the relationship between frailty and all-cause mortality in hypertensive population.We used data from National Health Nutrition Examination Survey (NHANES) 1999-2002 Death Index. Frailty was assessed using revised version of Fried criteria (weakness, exhaustion, low physical activity, shrinking, slowness). evaluate association mortality. Cox proportional hazard models were category mortality, adjusted for age, sex, race, education, poverty-income ratio,...
The endoplasmic reticulum (ER) membrane protein complex (EMC) is a conserved, multi-subunit acting as an insertase at the ER membrane. Growing evidence shows that EMC also involved in stabilizing and trafficking proteins. However, structural basis regulation of its multifunctionality remain elusive. Here, we report cryo-electron microscopy structures human apo- voltage-dependent anion channel (VDAC)-bound states resolutions 3.47 Å 3.32 Å, respectively. We discovered specific interaction...
Two types of cyanobacterial phycobilisomes (PBS) are present: the hemidiscoidal PBS (CpcG-PBS) and membrane-bound (CpcL-PBS). Both have ferredoxin:NADP+ oxidoreductase (FNR) attached to termini their rods through a CpcD domain. To date, physiological significance attachment remains unknown. We constructed mutant (dF338) which contains an FNR lacking N-terminal domain in Synechococcus sp. PCC 7002. Isolated CpcG-PBS from dF338 did not contain cell extracts had 35 kDa protein cross-reacting...