A5071502740- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Environmental DNA in Biodiversity Studies
- Microbial Community Ecology and Physiology
- Mitochondrial Function and Pathology
- Identification and Quantification in Food
- Biocrusts and Microbial Ecology
- Protist diversity and phylogeny
- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Genetic diversity and population structure
- Plant Stress Responses and Tolerance
- Metal-Catalyzed Oxygenation Mechanisms
- Species Distribution and Climate Change
- Wildlife Ecology and Conservation
- Plant and animal studies
- Plant Molecular Biology Research
- Metalloenzymes and iron-sulfur proteins
- Ecology and Vegetation Dynamics Studies
- Porphyrin Metabolism and Disorders
- RNA modifications and cancer
Peking University
2016-2025
Institute of Hydrobiology
2013-2025
Chinese Academy of Sciences
2015-2025
Zhejiang University of Technology
2025
State Key Laboratory of Plant Genomics
2025
Kunming Medical University
2024
Anhui University of Traditional Chinese Medicine
2024
Jiangxi Agricultural University
2022-2023
Institute of Hydrobiology, Biology Centre, Academy of Sciences of the Czech Republic
2020-2021
First Affiliated Hospital of Anhui Medical University
2021
Abstract Accurate assessments of fish species diversity and community composition are essential for understanding ecology conservation management. Environmental DNA (eDNA) metabarcoding has become an integrated method monitoring species. The accuracy efficacy eDNA rely heavily on the choice primers used PCR amplification. A wide selection been developed; however, there exists no comprehensive comparative evaluation their amplification or taxonomic classification a rich species, which hinders...
Abstract Here, we report our effort in generating an ORFeome collection for the Arabidopsis transcription factor (TF) genes. In total, clones representing 1,282 TF genes have been obtained Gateway high throughput cloning pENTR vector, including 411 whose annotation lack cDNA support. All inserts also mobilized into a yeast expression destination with estimated 85% rate of expressing respective proteins. Sequence analysis these revealed that 34 them did not match either reported cDNAs or...
Electron transfer rates to P700+ have been determined in wild-type and three interposon mutants (psaE-, ndhF-, psaE- ndhF-) of Synechococcus sp. PCC 7002. All grew significantly more slowly than wild type at low light intensities, each failed grow photoheterotrophically the presence 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) a metabolizable carbon source. The kinetics reduction were similar mutant whole cells absence DCMU. In DCMU, rate psaE was slower type. DCMU potassium cyanide, added...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPsaD is required for the stable binding of PsaC to photosystem I core protein Synechococcus sp. PCC 6301Ning Li, Jindong Zhao, Patrick V. Warren, Joseph T. Warden, Donald A. Bryant, and John H. GolbeckCite this: Biochemistry 1991, 30, 31, 7863–7872Publication Date (Print):August 1, 1991Publication History Published online1 May 2002Published inissue 1 August...
HetR plays a key role in regulation of heterocyst differentiation. When the Cys-48 residue from Anabaena sp. PCC 7120 was replaced with an Ala residue, mutant (HetR C48A ) could not dimerize, indicating that forms homodimer through disulfide bond. The strain C48, containing hetRc48a gene, produce and failed to form heterocyst. We show is DNA-binding protein its homodimerization required for DNA binding. binds promoter regions hetR , hepA patS suggesting direct control expression these genes...
Abstract Phycobilisomes (PBS) are the major light-harvesting machineries for photosynthesis in cyanobacteria and red algae they have a hierarchical structure of core peripheral rods, with both consisting phycobiliproteins linker proteins. Here we report cryo-EM structures PBS from two cyanobacterial species, Anabaena 7120 Synechococcus 7002. Both hemidiscoidal shape share common triangular structure. While has additional hexamers linked by 4th domain ApcE (L CM ). The predict that, compared...
Abstract Freshwater fish biodiversity is quickly decreasing and requires effective monitoring conservation. Environmental DNA (eDNA)‐based methods have been shown to be highly sensitive cost‐efficient for aquatic surveys, but few studies systematically investigated how spatial sampling design affects eDNA‐detected communities across lentic systems of different sizes. We compared the patterns diversity determined using eDNA in three lakes small (SL; 3 ha), medium (ML; 122 ha) large (LL; 4,343...
Globally, urbanization poses a major threat to terrestrial biodiversity, yet its impact on fish diversity is poorly understood, mainly because of surveying difficulties. In this study, environmental DNA metabarcoding was used survey communities at 109 lentic and lotic sites across Beijing, how variables affect biodiversity fine urban spatial scales investigated. We identified 52 native 23 non-native taxa, with waters harboring both common habitat-specific species. Water quality strongly...
Calcium and iron are essential elements that regulate many important processes of eukaryotic cells. Failure to maintain homeostasis calcium causes cell dysfunction or even death. PD (Parkinson’s disease) is the second most common neurological disorder in humans, for which there currently no viable treatment options effective strategies cure delay progression. Pathological hallmarks PD, such as dopaminergic neuronal death intracellular α-synuclein deposition, closely involved perturbations...
Environmental DNA (eDNA) technology has revolutionized biomonitoring, but challenges remain regarding water sample processing. The passive eDNA sampler (PEDS) represents a viable alternative to active, filtration-based enrichment methods, the effectiveness of PEDS for surveying biodiverse and complex natural bodies is unknown. Here, we collected using filtration glass fiber filter-based (submerged in 1 d) from 27 sites along final reach Yangtze River coast Yellow Sea, followed by...
Summary MreB is a bacterial actin that plays important roles in determination of cell shape and chromosome partitioning Escherichia coli Caulobacter crescentus . In this study, the mreB from filamentous cyanobacterium Anabaena sp. PCC 7120 was inactivated. Although null mutant showed drastic change shape, its growth rate, division filament length were unaltered. Thus, maintains but not required for partitioning. The wild type had eight 10 copies chromosomes per respectively. We demonstrated...
Increasing evidence shows that protein phosphorylation on serine (Ser), threonine (Thr), and tyrosine (Tyr) residues is one of the major post-translational modifications in bacteria, involved regulating a myriad physiological processes. Cyanobacteria are largest groups bacteria only prokaryotes capable oxygenic photosynthesis. Many cyanobacteria strains contain unusually high numbers kinases phosphatases with specificity Ser, Thr, Tyr residues. However, few dozen sites known, presenting...
Environmental DNA (eDNA) technology has revolutionized biomonitoring in recent years; however, eDNA collection from aquatic environments generally relies on the time-consuming and equipment-dependent process of water filtration. Passive sampling deploys sorbent materials to capture water, circumventing many problems associated with active filtration; yet, very few candidate have been systematically evaluated for this purpose. Here, we ability 12 different types common loose sorbents filter...
Phycobilisomes (PBS) are the major light harvesting complexes of photosynthesis in cyanobacteria and red algae. CpcL-PBS is a type small PBS that transfers energy directly to photosystem I without core structure. Here we report cryo-EM structure from cyanobacterium Synechocystis sp. PCC 6803 at 2.6-Å resolution. The shows CpcD domain ferredoxin: NADP+ oxidoreductase located distal end CpcL-PBS, responsible for its attachment PBS. With evidence ultrafast transient absorption fluorescence...
Abstract Background Floating bamboo ( Hygroryza aristata ) is an endangered species with a narrow native distribution and renowned for its unique aesthetic qualities, which holds significant ecological ornamental value. However, the lack of genetic information research, only one complete plastome available, significantly hampers conservation efforts further research this species. Results In we sequenced assembled organelle genomes floating bamboo, including mitogenome (587,847 bp) (135,675...