A5101785807- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Extracellular vesicles in disease
- Cell Adhesion Molecules Research
- Barrier Structure and Function Studies
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Atherosclerosis and Cardiovascular Diseases
- S100 Proteins and Annexins
- Cardiac Ischemia and Reperfusion
- Sepsis Diagnosis and Treatment
- Phagocytosis and Immune Regulation
- Blood Coagulation and Thrombosis Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Acute Kidney Injury Research
- Immune cells in cancer
- Traditional Chinese Medicine Analysis
- Blood properties and coagulation
- Gut microbiota and health
- Advanced Glycation End Products research
- Immune Response and Inflammation
- Chronic Kidney Disease and Diabetes
- Cancer-related molecular mechanisms research
- Neurological Disease Mechanisms and Treatments
- Cardiac Imaging and Diagnostics
- Neonatal and fetal brain pathology
- Plant Molecular Biology Research
University of South Florida
2000-2025
The Ohio State University
2024
Peking University
2004-2020
Institute of Genetics and Developmental Biology
2004-2020
Chinese Academy of Sciences
2004-2020
Florida College
2000-2017
State Administration of Traditional Chinese Medicine of the People's Republic of China
2015
Fudan University
2004
State Key Laboratory of Genetic Engineering
2004
Wuhan University
2004
Abstract Here, we report our effort in generating an ORFeome collection for the Arabidopsis transcription factor (TF) genes. In total, clones representing 1,282 TF genes have been obtained Gateway high throughput cloning pENTR vector, including 411 whose annotation lack cDNA support. All inserts also mobilized into a yeast expression destination with estimated 85% rate of expressing respective proteins. Sequence analysis these revealed that 34 them did not match either reported cDNAs or...
Endothelial hyperpermeability exacerbates multiple organ damage during inflammation or infection. The endothelial glycocalyx, a protective matrix covering the luminal surface of cells (ECs), undergoes enzymatic shedding inflammation, contributing to barrier hyperpermeability. A disintegrin and metalloproteinase 15 (ADAM15) is sheddase capable cleaving ectodomains membrane-bound molecules. Herein, we tested whether how ADAM15 involved in glycocalyx vascular leakage sepsis. Dextran-150kD...
Severe lung infection can lead to brain dysfunction and neurobehavioral disorders. The mechanisms that regulate the lung-brain axis of inflammatory response respiratory are incompletely understood. This study examined effects causing systemic neuroinflammation as a potential mechanism contributing blood-brain barrier (BBB) leakage behavioral impairment.Lung in mice was induced by instilling Pseudomonas aeruginosa (PA) intratracheally. We determined bacterial colonization tissue,...
Abstract Endothelial dysfunction is a hallmark of systemic inflammatory response underlying multiple organ failure. Here we report novel function DHHC-containing palmitoyl acyltransferases (PATs) in mediating endothelial inflammation. Pharmacological inhibition PATs attenuates barrier leakage and leucocyte adhesion induced by junction hyperpermeability ICAM-1 expression during Among 11 DHHCs detected vascular endothelium, DHHC21 required for response. Mice with deficiency ( Zdhhc21 dep/dep )...
Extracellular vesicles (EVs) have attracted rising interests in the cardiovascular field not only because they serve as serological markers for circulatory disorders but also participate important physiological responses to stress and inflammation. In circulation, these membranous are mainly derived from blood or vascular cells, carry cargos with distinct molecular signatures reflecting origin activation state of parent cells that produce them, thus providing a powerful tool diagnosis...
Abstract Aims Microvesicles (MVs) conduct intercellular communication and impact diverse biological processes by transferring bioactive cargos to other cells. We investigated whether how endothelial production of MVs contribute vascular dysfunction during inflammation. Methods results measured the levels molecular properties endothelial-derived (EC-MVs) from mouse plasma following a septic injury elicited cecal ligation puncture, as well those supernatants cultured cells stimulated...
Salt and drought-induced RING finger1 (SDIR1) is a RING-type E3 ubiquitin ligase that plays key role in ABA-mediated responses to salinity drought stress via the ubiquitination pathway some plant species. However, its function wheat (Triticum aestivum) unknown. Here, we isolated SDIR1 member wheat, TaSDIR1-4A, characterized activity. DNA polymorphism assays showed presence of two nucleotide variation sites promoter region leading detection haplotypes Hap-4A-1 Hap-4A-2 populations....
Blood-brain barrier (BBB) dysfunction occurs in numerous central nervous system disorders. Unfortunately, a limited understanding of the mechanisms governing function hinders identification and assessment BBB-targeted therapies. Previously, we found that non-muscle myosin light chain kinase (nmMLCK) negatively regulates tight junction protein claudin-5 brain microvascular endothelial cells (BMVECs) under inflammatory conditions. Here, used complementary animal primary cell co-culture models...
Gut ischemia/reperfusion (I/R) injury is a common clinical problem associated with significant mortality and morbidities that result from systemic inflammation remote organ dysfunction, typically acute lung injury. The mechanisms underlying the dissemination of gut-derived harmful mediators into circulation are poorly understood. objective our study was to determine role mesenteric lymphatic in pulmonary inflammatory response gut I/R. Using murine intestinal I/R model, we evaluated whether...
Lymphopenia, a reduction in blood lymphocyte count, is known to inversely correlate with the prognosis for patients myocardial infarction (MI). However, underlying mechanisms by which cardiac ischemia induces lymphopenia remain elusive. This study provides first evidence that MI activates hypothalamic-pituitary-adrenal (HPA) axis increase glucocorticoid secretion, and elevated circulating glucocorticoids induce lymphocytes trafficking bone marrow, leading lymphopenia.
QiShenYiQi Pills (QSYQ) is a compound Chinese medicine used for treatment of cardiovascular diseases. The present study investigated the effects QSYQ on Doxorubicin- (DOX-) induced disorders in rat cardiac structure and function possible mechanism underlying. A total 24 male Sprague-Dawley rats were administrated by intraperitoneal injections with DOX at dose 2.5 mg/kg, once every day 6 times. After 6th injection, evaluated echocardiographic analysis, animals injured heart (n = 14) divided...
Inflammation-induced blood–brain barrier (BBB) dysfunction and microvascular leakage are associated with a host of neurological disorders. The tight junction protein claudin-5 (CLDN5) is crucial necessary for BBB integrity maintenance. CLDN5 negatively regulated by the transcriptional repressor FOXO1, whose activity increases during impaired insulin/AKT signaling. Owing to an incomplete understanding mechanisms that regulate expression in maintenance dysfunction, therapeutic interventions...
Abstract The present study aimed to detect the role of 3, 4-dihydroxyl-phenyl lactic acid (DLA) during ischemia/reperfusion (I/R) induced myocardial injury with emphasis on underlying mechanism DLA antioxidant. Male Spragu-Dawley (SD) rats were subjected left descending artery occlusion followed by reperfusion. Treatment ameliorated structure and function disorder, blunted impairment Complex I activity mitochondrial after I/R. results 2-D fluorescence difference gel electrophoresis revealed...
Objective The endothelial glycocalyx constitutes part of the barrier but its degradation leaves cells exposed to transmigrating and circulating mediators that can damage or promote intercellular gaps. Syndecan proteins are key components shed during disease states where expression activity proteases such as thrombin elevated. We tested ability cleave ectodomains syndecans whether products could act directly on alter function. Approach results Using transmission electron microscopy, we...
Background/Aims: Acute kidney injury (AKI) is a major complication of transplantation, resulting in early graft dysfunction. Since diuretic acetazolamide (AZA) has been shown to improve contrast induced AKI, we hypothesized that AZA also protected against ischemia-reperfusion (I/R) caused AKI. Methods: An vivo mouse renal I/R model and an vitro H2O2 stimulated HK-2 cell were utilized examine the renoprotective effect AZA. Renal blood flow measured. Nitric oxide synthase (eNOS)/Nitric (NO),...
Cardiotonic pill (CP) is a compound Chinese medicine currently used in China for treatment of ischemic angina pectoris. Our previous results indicated that single dosing CP pretreatment at 0.8 g/kg attenuates ischemia/reperfusion- (I/R-) induced myocardial injury and cardiac microcirculatory disturbance. The present study aimed to investigate the effect low dosage multiple manner uncover mechanism antioxidative activity CP. Male Sprague-Dawley rats were subjected left anterior descending...
Lipopolysaccharide (LPS) causes microvascular barrier disruption, leading to albumin leakage from microvessels resulting in a range of disastrous sequels. Salvianolic acid B (SalB) is major water-soluble component derived Salvia miltiorrhiza. Previous studies showed its potential attenuate dysfunction, but the underlying mechanism not fully understood. The present study was intended investigate impact SalB on endothelial cell vivo rat mesenteric venules as well vitro human umbilical vein...
Extracellular vesicles (EVs) are nano-sized membrane-bound particles containing biologically active cargo molecules. The production and molecular composition of EVs reflect the physiological state parent cells, once released into circulation, they exert pleiotropic functions via transferring contents. Thus, circulating not only serve as biomarkers, but also mediators in disease processes or injury responses. In present study, we performed a comprehensive analysis plasma from burn patients...