A5060275560- Cell Adhesion Molecules Research
- Osteoarthritis Treatment and Mechanisms
- Protease and Inhibitor Mechanisms
- Proteoglycans and glycosaminoglycans research
- Connective tissue disorders research
- TGF-β signaling in diseases
- Collagen: Extraction and Characterization
- Spaceflight effects on biology
- Influenza Virus Research Studies
- Graphene and Nanomaterials Applications
- Cytokine Signaling Pathways and Interactions
- Bone and Dental Protein Studies
- Tissue Engineering and Regenerative Medicine
- Cellular Mechanics and Interactions
- Biomarkers in Disease Mechanisms
- Chemotherapy-induced cardiotoxicity and mitigation
- Barrier Structure and Function Studies
- Plasma Applications and Diagnostics
- S100 Proteins and Annexins
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
- HIV Research and Treatment
- Cardiac Fibrosis and Remodeling
- Hippo pathway signaling and YAP/TAZ
Boise State University
2016-2025
Matrix Research (United States)
2021-2023
Molecular Research Institute
2021
Royal London Hospital
1988-2008
Oregon Health & Science University
1994-2007
Queen Mary University of London
2001-2005
Vector Control Research Centre
2005
Open International University for Alternative Medicines
2005
Colorado State University
2001-2003
Shriners Hospitals for Children - Portland
1994-1998
The current study investigated healing of large full-thickness articular cartilage defects during the first 8 weeks with and without penetration subchondral bone using microfracture in an established equine model healing. Chondral weightbearing portion medial femoral condyle were made bilaterally; one defect each horse was microfractured whereas contralateral leg served as control. expression extracellular matrix components (Types I II collagen aggrecan) evaluated histologic techniques,...
Protein aggregation is a hallmark of several neurodegenerative diseases and also cataracts. The major proteins in the lens eye are crystallins, which accumulate throughout life extensively modified. Deamidation modification during aging Among betaA3-subunit has been found to have multiple sites deamidation associated with insoluble vivo. Several were predicted be exposed on surface betaA3 investigated this study. was mimicked by site-directed mutagenesis at Q42 N54 N-terminal domain, N133...
S100A9, an alarmin that can form calprotectin (CP) heterodimers with S100A8, is mainly produced by keratinocytes and innate immune cells. The contribution of keratinocyte-derived S100A9 to psoriasis (Ps) psoriatic arthritis (PsA) was evaluated using mouse models, the potential usefulness as a Ps/PsA biomarker assessed in patient samples.
The collagen fibrils of hyaline cartilage vary in diameter depending on developmental stage and location within the tissue. In general, growth plates fetal epiphyseal cartilages contain with diameters less than approximately 25 nm, whereas permanent adult tissues contains 30-200 nm. interstitial are complex, having at least three types as integral components. Type XI, a member fibrillar class, has been proposed to limit fibril diameter. To test this proposition we sought determine if XI was...
Mesenchymal stem cells (MSC) rely on their ability to integrate physical and spatial signals at load bearing sites replace renew musculoskeletal tissues. Designed mimic unloading experienced during spaceflight, preclinical simulated microgravity models show that alteration of gravitational loading limits proliferative activity cells. Emerging evidence indicates this loss proliferation may be linked cellular cytoskeleton contractility. Low intensity vibration (LIV) is an exercise mimetic...
The amino-terminal domain of collagen α1(XI) plays a key role in controlling fibrillogenesis. However, the specific mechanisms through which various isoforms regulate this process are not fully understood. We measured kinetics type I self-assembly presence isoforms. Molecular dynamics simulations, protein–protein docking studies, and molecular mechanics Poisson–Boltzmann surface area were utilized to understand mechanisms. In vitro, silico, thermodynamic studies demonstrated an...
Blood-brain barrier (BBB) dysfunction occurs in numerous central nervous system disorders. Unfortunately, a limited understanding of the mechanisms governing function hinders identification and assessment BBB-targeted therapies. Previously, we found that non-muscle myosin light chain kinase (nmMLCK) negatively regulates tight junction protein claudin-5 brain microvascular endothelial cells (BMVECs) under inflammatory conditions. Here, used complementary animal primary cell co-culture models...
Collagen XI alpha 1 (Col11a1) is an extracellular matrix molecule required for embryonic development with a role in both nucleating the formation of fibrils and regulating diameter heterotypic during collagen fibrillar assembly. Although found many different tissues throughout vertebrate body, Col11a1 plays essential endochondral ossification. To further understand function process bone formation, we compared skeletal mineralization wild-type (WT) mice Col11a1-deficient using X-ray...
Abstract Osteoarthritis is a degenerative joint disease that limits mobility of the affected due to degradation articular cartilage and subchondral bone. The limited regenerative capacity presents significant challenges when attempting repair or reverse effects degradation. Tissue engineered medical products are promising alternative treat osteochondral degeneration their potential integrate into patient's existing tissue. goal this study was create scaffold would induce site‐specific...
Abstract Reducing the musculoskeletal deterioration that astronauts experience in microgravity requires countermeasures can improve effectiveness of otherwise rigorous and time-expensive exercise regimens space. The ability low-intensity vibrations (LIV) to activate force-responsive signaling pathways cells suggests LIV as a potential countermeasure cell responsiveness subsequent mechanical challenge. Mechanoresponse mesenchymal stem (MSC), which maintain bone-making osteoblasts, is part...
To determine concentrations of matrix metalloproteinase (MMP)-2 and -9 in synovial fluid; mRNA expression MMP-1, -13, -3; interleukin[IL]-1alpha beta; tumor necrosis factor (TNF)-alpha membrane articular cartilage from horses with naturally occurring joint disease.Synovial fluid (n = 76), (59), (45) 5 clinically normal 55 disease categorized as traumatic (acute [AT] or chronic [CT]), osteochondritis dissecans (OCD), septic (S).Synovial gelatinase were analyzed, using zymography. Synovial for...
Collagen XI is a heterotrimeric molecule found predominantly in heterotypic cartilage fibrils, where it involved the regulation of fibrillogenesis. This function thought to involve complex N-terminal domain. The goal this current study was examine its structural organization further elucidate regulatory mechanism. amino-propeptide (α1-Npp) alone or with isoforms variable region were recombinantly expressed and purified by affinity molecular sieve chromatography. Cys-1–Cys-4 Cys-2–Cys-3...
According to the World Health Organization, cataracts account for half of blindness in world, with majority occurring developing countries. A cataract is a clouding lens eye due light scattering precipitated proteins or aberrant cellular debris. The major are crystallins, and they extensively deamidated during aging cataracts. Deamidation has been detected at domain monomer interfaces several crystallins aging. purpose this study was determine effects two potential deamidation sites...